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Variations of root traits in three Xizang grassland communities along a precipitation gradient
ZHOU Wei, LI Hong-Bo, ZENG Hui
Chin J Plant Ecol    2018, 42 (11): 1094-1102.   DOI: 10.17521/cjpe.2018.0140
Abstract   (1108 HTML131 PDF(pc) (1188KB)(1032)  

Aims Root functional traits and their variations mediate coexistence and adaptive strategy of plant species. Yet, strong environmental constraints may induce convergence of root traits among different plant species. To study the variations of root traits and clarify the diverse adaptive strategies across plant species, we sampled three alpine grasslands along a precipitation gradient in the Xizang Plateau.
Methods In three grassland communities along a precipitation gradient: Nagqu, Baingoin and Nyima from east to west of Xizang Plateau, we collected 22 coexisting plant species and measured three key root traits: 1st-order root diameter, 1st-order lateral root length and root branch intensity.
Important findings The main results showed that: (1) the root of plants in the alpine grassland was generally thin, and the interspecific variation was also small (22.76%); (2) the root diameter of 86% plant species was in the range from 0.073 mm to 0.094 mm. Compared with the thick-root species, thin-root species had a higher root branching intensity, but shorter lateral root length. In addition, at community-level, plants mainly increased root diameter and lateral root length, but reduced root branching intensity to adapt to the decreasing precipitation; while at species-level, the plant species exhibited diverse adaptive strategies along the precipitation gradient.


Fig. 3 Root trait mean values of seven regionally common species (appearing in two or three sites at the same time) at three grassland sites (mean + SE) in Xizang alpine grassland. Ad, Artemisia demissa; Ts, Trisetum spicatum; Lp, Leontopodium pusillum; Sp, Stipa purpurea; Pb, Potentilla bifurca; Om, Oxytropis microphylla; Hs, Heteropappus semiprostratus.
Extracts from the Article
在实验室中用去离子水洗去附着在根上的细小杂质与灰尘, 并将其置于放有去离子水的培养皿中(使根系间不会重叠), 利用扫描仪(Expression 10000XL; Epson, Suwa, Japan)进行扫描, 并使用WINRHIZO软件(Regent Instruments, Quebec, Canada)将获取的图像进行处理分析, 获得一级根直径、一级根长度及分支强度3个指标。其中根系的分级是严格遵循Fitter (1987)、Berntson (1997)及Pregitzer等(2002)所描述的分级方法, 即将最末梢的根系命名为一级根。此外, 对于每一个根系图像, 人工计数每个二级根上的一级根数量, 进而利用一级根数量除以二级根的长度计算出根系分支强度(Kong et al., 2014)。
区域常见物种往往分布在不同的草原类型中, 其根系属性对区域水分变化的响应方向及幅度有所差异。结果显示, 随着水分减少, 一级根较粗物种的直径变化要大于一级根较细物种, 但一级根较细物种的其他根系属性变化程度要高于一级根较粗物种(图3)。对于直径来说, 一级根较粗的小叶棘豆和半卧狗娃花随水分减少增加了32%-36%, 而一级根较细物种中只有二裂委陵菜(Potentilla bifurca)随水分减少提高了13%-14%, 其他一级根较细物种直径随水分减少并未有显著变化; 相对于降水较多的班戈地区, 一级根较细物种(弱小火绒草(Leontopodium pusillum))一级侧根长度在降水较少的尼玛地区增长13%-97%, 而一级根较粗物种(半卧狗娃花)则仅增长了8%; 同样, 在水分减少情况下, 一级根较细物种中的弱小火绒草和紫花针茅的根系分支强度降低了45%-69%, 而一级根较粗物种中只有半卧狗娃花的根系分支强度有所变化, 且仅降低了23%, 但纤杆蒿的根分枝强度随水分减少的变化具有特殊性, 呈现了相反的趋势, 其根系分支强度随水分的减少反而增加了123%。
除了物种间的变化, 不同群落里共有种的属性的种内变异可能也会影响群落CWM值(Violle et al., 2012; Kichenin & Freschet, 2013)。我们的结果显示, 随水分含量降低, 根直径相对于其他根属性来说几乎没有变化, 但侧根长度和分支强度均有所增加, 尤其是分支强度(图4)。另外, 我们也发现, 不同共有种根系属性响应水分变化的规律并不一致(图3)。不同物种的属性种内变异对环境梯度的响应具有较大异质性(Albert et al., 2010; Kichenin & Freschet, 2013)。在区域常见的7个物种中, 半卧狗娃花对水分变化的响应与群落整体尺度上物种的响应一致, 3个根系属性均有所改变; 弱小火绒草主要通过增加侧根长度及降低分枝强度两个属性来适应环境水分的变化; 小叶棘豆、二裂委陵菜、紫花针茅及纤杆蒿则主要通过改变一个属性值的方式来响应水分变化; 而西藏三毛草3个根系属性随环境水分的变化均未有显著变化。以上说明植物的根系属性是会随着环境水分的变化而变化的, 且植物往往是通过改变单一属性或多个属性的方式来适应环境的变化(Nosil et al., 2009), 而物种间这种通过协调不同属性及属性组合间的变异方向及程度来适应水分减少的方式, 体现了不同物种适应环境能力及策略的多样性(Laughlin & Messier, 2015)。
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