榕属植物特定挥发物决定榕管蓟马的寄主选择行为

doi:10.17521/cjpe.2022.0121

植物生态学报 ›› 2023, Vol. 47 ›› Issue (7): 954-966.DOI: 10.17521/cjpe.2022.0121

榕属植物特定挥发物决定榕管蓟马的寄主选择行为

黄鹏¹, 林勇文², 张杰¹, 姚锦爱¹, 余德亿¹^,^*()

¹福建省作物有害生物监测与治理重点实验室, 福建省农业科学院植物保护研究所, 福建省作物有害生物绿色防控工程研究中心, 福州 350013
²漳州职业技术学院, 福建漳州 363000

收稿日期:2022-04-06 接受日期:2022-09-28 出版日期:2023-07-20 发布日期:2023-07-21
通讯作者: *余德亿(yudy_2004@126.com)
作者简介:ORCID: 黄鹏: 0000-0001-6231-9891
基金资助:
福建省省属公益类科研院所基本科研专项(2018R1025-2);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021011);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021017)

Specific volatiles of Ficus plants determine host preference behavior of Gynaikothrips uzeli

HUANG Peng¹, LIN Yong-Wen², ZHANG Jie¹, YAO Jin-Ai¹, YU De-Yi¹^,^*()

¹Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fujian Engineering Research Center for Green Pest Management, Fuzhou 350013, China
²Zhangzhou Institute of Technology, Zhangzhou, Fujian 363000, China

Received:2022-04-06 Accepted:2022-09-28 Online:2023-07-20 Published:2023-07-21
Contact: *YU De-Yi(yudy_2004@126.com)
Supported by:
The Special Fund for Agro-scientific Research in the Public Interest of Fujian Province(2018R1025-2);The “5511” Collaborative Innovation Project of High-quality Agricultural Development and Surpassment in Fujian Province(XTCXGC2021011);The “5511” Collaborative Innovation Project of High-quality Agricultural Development and Surpassment in Fujian Province(XTCXGC2021017)

摘要/Abstract

摘要：

榕管蓟马(Gynaikothrips uzeli)是榕属(Ficus)植物上的一种重要检疫性害虫, 为明确该昆虫对榕属植物的选择行为及决定这种行为的关键因素, 该研究通过昆虫行为测试, 分析该昆虫对3种不同盆栽榕树(Ficus spp.)挥发物的行为反应及其与挥发物相对含量的相关性, 筛选并验证决定选择行为的挥发物成分, 结合相关基因表达量, 推断合成这些特定挥发物的关键基因, 为寻找绿色、有效和持久的防控措施提供依据。结果表明, 榕管蓟马对3种盆栽榕树挥发物的趋向性以垂叶榕(F. benjamina)最强, 人参榕(F. microcarpa)其次, 印度榕(F. elastica)最弱; 这种行为反应与挥发物中β-环柠檬醛、β-马榄烯、β-古巴烯的相对含量呈显著正相关关系, 与反-α-香柑油烯的相对含量呈显著负相关关系; 3种盆栽榕树中, β-环柠檬醛的相对含量与基因Fm.09G0004550 (CCD4)的表达量呈显著正相关关系, β-马榄烯的相对含量与基因Fm.newGene1857 (STPS)、Fm.newGene6827 (VIT_19s0014g04930)的表达量呈显著正相关关系, β-古巴烯的相对含量与基因Fm.11G0000860 (LUP2)、Fm.newGene1857 (STPS)的表达量呈显著正相关关系, 反-α-香柑油烯的相对含量与基因Fm.13G0003820 (LUS1)、Fm.newGene489 (GgbAS1)的表达量呈显著正相关关系。综上, 榕属植物上决定榕管蓟马行为反应的特定挥发物主要为β-环柠檬醛、β-马榄烯、β-古巴烯和反-α-香柑油烯, 这4种萜类挥发物合成可能主要与6个萜烯合成酶基因Fm.09G0004550、Fm.11G0000860、Fm.newGene1857、Fm.newGene6827、Fm.13G0003820和Fm.newGene489有关。

关键词: 榕属, 萜类挥发物, 榕管蓟马, 行为反应, 萜烯合成酶基因

Abstract:

Aims Gynaikothrips uzeli is a major quarantine pest attacking Ficusplants. To seek effective and sustainable methods to control the pest, it is necessary to assess its host preference among different Ficusspecies and understand the critical factors of this behavior.

Methods In this study, the behavioral preference of G. uzeli to volatile organic compounds (VOCs) of three potted Ficusspecies were evaluated. The VOCs released by the three Ficus species were analyzed using GC-MS, and the major compnents were used to test the pest preference. Based on the transcriptome analyses, we inferred the key genes that affect the biosyntheses of those VOCs.

Important findings Behavioral preference of G. uzeli to the VOCs of the three Ficusspecies is F. benjamina > F. microcarpa > F. elastica.The behavioral preference of G. uzeliwas significantly positively related to the relative content of β-cyclocitral, β-maaliene, β-copaene, and significantly negatively related to the relative content of trans-α-bergamotene. A significantly positive relationship was found between the relative content of β-cyclocitral and the expression of gene Fm.09G0004550 (CCD4). Moreover, the relative content of β-maaliene was significantly positively related to the expression of gene Fm.newGene1857(STPS) and Fm.newGene6827 (VIT_19s0014g04930). The relative content of β-copaene was significantly positively related to the expression of gene Fm.11G0000860 (LUP2) and Fm.newGene1857 (STPS). The relative content of trans-α-bergamotene was significantly positively associated with the expression of gene Fm.13G0003820 (LUS1) and Fm.newGene489 (GgbAS1). The above findings suggest that four terpenes (β-cyclocitral, β-maaliene, β-copaene and trans-α-bergamotene) determine the behavioral preference of G. uzeli to Ficus plants, and their biosyntheses might be mainly affected by six terpene synthase genes (Fm.09G0004550, Fm.11G0000860, Fm.newGene1857, Fm.newGene6827, Fm.13G0003820 and Fm.newGene489).

Key words: Ficus, terpene volatiles, Gynaikothrips uzeli, behavioral preference, terpene synthase genes

黄鹏, 林勇文, 张杰, 姚锦爱, 余德亿. 榕属植物特定挥发物决定榕管蓟马的寄主选择行为. 植物生态学报, 2023, 47(7): 954-966. DOI: 10.17521/cjpe.2022.0121

HUANG Peng, LIN Yong-Wen, ZHANG Jie, YAO Jin-Ai, YU De-Yi. Specific volatiles of Ficus plants determine host preference behavior of Gynaikothrips uzeli. Chinese Journal of Plant Ecology, 2023, 47(7): 954-966. DOI: 10.17521/cjpe.2022.0121

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2022.0121

https://www.plant-ecology.com/CN/Y2023/V47/I7/954

图/表 9

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基因 Gene	正向引物 Forward sequence	反向引物 Reverse sequence	基因描述 Gene description
Fm.09G0004550	ACTTTCGTGGTGATGGAT	GTCCGCTTCCTTGATAAAG	参与类胡萝卜素生物裂解, 编码类胡萝卜素裂解双加氧酶CCD4 Participates in carotenoid biolysis, encodes the carotenoid cleavage dioxygenase CCD4
Fm.11G0000860	GAACTCTCAACACTTGGTT	CACCCAATTATCGCCAAT	参与倍半萜和三萜生物合成, 编码倍半萜合成酶LUP2 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase LUP2
Fm.newGene1857	AGAGGAAGAAGCGGTTGAAGA	CATTGGAACACTGATTGGGTAGAG	参与倍半萜生物合成, 编码倍半萜合成酶STPS Participates in sesquiterpene biosynthesis, encodes the sesquiterpene synthetase STPS
Fm.newGene6827	GCTAATGAAGAATGCCTCCATA	GCCATCTCCATCTAAGTACATC	参与倍半萜生物合成, 编码倍半萜合成酶VIT 19s0014g04930 Participates in sesquiterpene biosynthesis, encodes the sesquiterpene synthetase VIT 19s0014g04930
Fm.13G0003820	TCCAGACTCTGGTCCTCTTTAG	CATGAACCATCAGGGTTTTGC	参与倍半萜和三萜生物合成, 编码倍半萜合成酶LUS1 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase LUS1
Fm.newGene489	TAACCATCGGATGTGTTGAG	CTCTCGGAATATGCTTCTTGA	参与倍半萜和三萜生物合成, 编码倍半萜合成酶GgbAS1 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase GgbAS1
max2	CGGAGTTCTACTACTGGACGG	GGGACAAGCCTCGGTGAT	内参基因, 编码蛋白F-box Reference gene, encodes the protein F-box

基因 Gene	正向引物 Forward sequence	反向引物 Reverse sequence	基因描述 Gene description
Fm.09G0004550	ACTTTCGTGGTGATGGAT	GTCCGCTTCCTTGATAAAG	参与类胡萝卜素生物裂解, 编码类胡萝卜素裂解双加氧酶CCD4 Participates in carotenoid biolysis, encodes the carotenoid cleavage dioxygenase CCD4
Fm.11G0000860	GAACTCTCAACACTTGGTT	CACCCAATTATCGCCAAT	参与倍半萜和三萜生物合成, 编码倍半萜合成酶LUP2 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase LUP2
Fm.newGene1857	AGAGGAAGAAGCGGTTGAAGA	CATTGGAACACTGATTGGGTAGAG	参与倍半萜生物合成, 编码倍半萜合成酶STPS Participates in sesquiterpene biosynthesis, encodes the sesquiterpene synthetase STPS
Fm.newGene6827	GCTAATGAAGAATGCCTCCATA	GCCATCTCCATCTAAGTACATC	参与倍半萜生物合成, 编码倍半萜合成酶VIT 19s0014g04930 Participates in sesquiterpene biosynthesis, encodes the sesquiterpene synthetase VIT 19s0014g04930
Fm.13G0003820	TCCAGACTCTGGTCCTCTTTAG	CATGAACCATCAGGGTTTTGC	参与倍半萜和三萜生物合成, 编码倍半萜合成酶LUS1 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase LUS1
Fm.newGene489	TAACCATCGGATGTGTTGAG	CTCTCGGAATATGCTTCTTGA	参与倍半萜和三萜生物合成, 编码倍半萜合成酶GgbAS1 Participates in sesquiterpene and triterpene biosynthesis, encodes the sesquiterpene synthetase GgbAS1
max2	CGGAGTTCTACTACTGGACGG	GGGACAAGCCTCGGTGAT	内参基因, 编码蛋白F-box Reference gene, encodes the protein F-box

挥发物成分 VOCs name	相对含量 Relative content (%)			r	p
挥发物成分 VOCs name	垂叶榕 F. benjamina	人参榕 F. microcarpa	印度榕 F. elastica	r	p
β-环柠檬醛 β-cyclocitral	2.84 ± 013^a	2.32 ± 0.32^ab	0.96 ± 0.50^b	0.999 5	0.020 0
β-马榄烯 β-maaliene	0.37 ± 0.05^a	0.29 ± 0.05^a	0.00 ± 0.00^b	0.996 0	0.044 2
β-古巴烯 β-copaene	0.12 ± 0.02^a	0.08 ± 0.03^ab	0.00 ± 0.00^b	0.999 3	0.023 4
胆甾-3, 5-二烯 Cholesta-3,5-diene	0.00 ± 0.00^c	0.43 ± 0.21^b	4.26 ± 1.44^a	-0.979 1	0.093 7
顺-α-香柑油烯 Cis-α-bergamotene	0.00 ± 0.00^b	0.14 ± 0.14^b	3.02 ± 0.57^a	-0.967 5	0.094 8
反-11-十八烯酸甲酯 Trans-11-octadecenoate	0.00 ± 0.00^b	0.19 ± 0.10^ab	0.45 ± 0.10^a	-0.990 6	0.087 4
反-α-香柑油烯 Trans-α-bergamotene	0.00 ± 0.00^c	0.13 ± 0.07^b	0.43 ± 0.06^a	-1.000 0	0.001 6

挥发物成分 VOCs name	相对含量 Relative content (%)			r	p
挥发物成分 VOCs name	垂叶榕 F. benjamina	人参榕 F. microcarpa	印度榕 F. elastica	r	p
β-环柠檬醛 β-cyclocitral	2.84 ± 013^a	2.32 ± 0.32^ab	0.96 ± 0.50^b	0.999 5	0.020 0
β-马榄烯 β-maaliene	0.37 ± 0.05^a	0.29 ± 0.05^a	0.00 ± 0.00^b	0.996 0	0.044 2
β-古巴烯 β-copaene	0.12 ± 0.02^a	0.08 ± 0.03^ab	0.00 ± 0.00^b	0.999 3	0.023 4
胆甾-3, 5-二烯 Cholesta-3,5-diene	0.00 ± 0.00^c	0.43 ± 0.21^b	4.26 ± 1.44^a	-0.979 1	0.093 7
顺-α-香柑油烯 Cis-α-bergamotene	0.00 ± 0.00^b	0.14 ± 0.14^b	3.02 ± 0.57^a	-0.967 5	0.094 8
反-11-十八烯酸甲酯 Trans-11-octadecenoate	0.00 ± 0.00^b	0.19 ± 0.10^ab	0.45 ± 0.10^a	-0.990 6	0.087 4
反-α-香柑油烯 Trans-α-bergamotene	0.00 ± 0.00^c	0.13 ± 0.07^b	0.43 ± 0.06^a	-1.000 0	0.001 6

挥发物成分 VOCs name	基因名 (注释) Gene name (annotation)	r	p
β-环柠檬醛 β-cyclocitral	Fm.09G0004550 (CCD4)	0.998 3	0.037 5
β-马榄烯 β-maaliene	Fm.11G0000860 (LUP2)	0.988 0	0.098 7
	Fm.newGene1857 (STPS)	0.997 8	0.042 0
	Fm.newGene6827 (VIT_19s0014g04930)	0.999 8	0.019 1
β-古巴烯 β-copaene	Fm.11G0000860 (LUP2)	0.999 6	0.018 1
	Fm.newGene1857 (STPS)	0.998 2	0.038 6
	Fm.newGene6827 (VIT_19s0014g04930)	0.987 8	0.099 7
反-α-香柑油烯 Trans-α-bergamotene	Fm.13G0003820 (LUS1)	0.997 6	0.044 3
反-α-香柑油烯 Trans-α-bergamotene	Fm.newGene489 (GgbAS1)	0.999 2	0.025 0

榕属植物特定挥发物决定榕管蓟马的寄主选择行为

Specific volatiles of Ficus plants determine host preference behavior of Gynaikothrips uzeli

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