植物生态学报 ›› 1992, Vol. 16 ›› Issue (4): 311-316.

• 论文 • 上一篇    下一篇

芦苇耐脱水能力的生理生态学分析

张承烈,周瑞莲,陈国仓   

  • 发布日期:1992-04-10
  • 通讯作者: 张承烈

Physio-Ecological Analysis on the Capability of Dehydration Tolerance of Phragmites communis

Zhang Cheng-lie, Zhou Rui-lian, Chen Guo-cang   

  • Published:1992-04-10
  • Contact: Zhang Li-quan

摘要: 甘肃省河西走廊分布有不同生态环境的芦苇,包括沼泽芦苇、盐化草甸芦苇和沙丘芦苇。盐化草甸芦苇和沙丘芦苇表观出很强的耐脱水能力。 测定与植物耐脱水能力相关的SOD、CAT和POD活力,表现为SOD活力以沙丘芦苇最高,盐化草甸芦苇次之,沼泽芦苇最低,但CAT和POD则以沼泽芦苇高于其它生境芦苇。用聚丙烯酰胺垂直板电泳方法分析这3种酶同工酶谱,结果表明,3种不同生境芦苇的SOD同工酶谱基本相同,均有9条酶带,但沙丘芦苇和盐化草甸芦苇的CAT和POD同工酶带数比沼泽芦苇分别增多3条和2条。这些结果证明,芦苇在自然选择压力下形成的耐脱水能力与基因调控的酶多型性相关。

Abstract: Reed plant (Phragmites communis)are perennial grasses growing in swamp, salt meadow and sand dune in the Hexi Corridor, Gansu province. Salt meadow-reed and sand dune-reed are capable of tolerating dehydration caused by water stress. The activities of catalase (CAT), peroxidase (POD) and superroxide dismutase (SOD) being related to dehydration tolerance of plants were determined. The results showed that the SOD activity in leaves of sand dunereed is the highest, in salt meadow-reed comes second, in swampreed is the minimum. However, the activities of CAT and POD in leaves of swamp- reed is higher than that in leaves of other habitat reeds. The isozyme made up of CAT, POD and SOD from leaves of different habitat reed were analyzed by polyacrylamide gel electrophoresis. The SOD from leaves of salt meadow- reed and sand dune-reed compared with swamp-reed, have no variation in isozyme patterns. However, differences were observed in the different habitat reed. CAT and POD from leaves of swamp-reed have 7 band and 6 bands, respectively. Sand dune-reed has 10 CAT bands and 9 POD bands, salt meadow-reed has 9 CAT bands and 8 POD bands. These results demonstrated that the dehydration tolerance of reed plants may be attributed to enzyme polymorphismus being controlled by gene.