Abstract: Spring wheat (Triticum aestivvm cv. "3131") was subject to three soil water regimes in the tanks of lysimeter at northern part of Linze Oasis which is located in the Hexi Corridor, The observations of evapotranspiration (ET) ,transpiration of single leaf, various ecological factors and some biological parameters were conducted in both typical fine day and overcast day and during the whole life span of wheat. The results showed that the total ET of spring wheat during its life span were 593mm, 365mm and 280mm for sufficient water supply, moderate water supply and insufficient water supply, respectively, and the average daily ETs for the three soil regimes were 5.5mm, 3.4mm and 2.8mm. The possible impacts of various biological and ecological factors on ET and wheat leaf transpiration were presented in the paper. Regression and correlation analysis demonstrated that the order of correlation coefficients of various biological factors with leaf transpiration was leaf water potential>leaf stomatal conductance>leaf temperature for sufficient water supply, and leaf water potential>leaf temperature>leaf stomatal conductance for other two soil water regimes; and the order of correlation coefficients of various ecological factors with ET is photosynthetically active radiation>net irradiance>soil temperature in 20cm depth, and air water vapor deficit>soil temperature in 15cm depth, while ambient temperature>soil temperature in 10cm, 0cm and 5cm depths.