绿叶挥发物产生特征及其生态生理作用研究进展

doi:10.3724/SP.J.1258.2013.00027

摘要/Abstract

摘要：

该文系统综述了植物绿叶挥发物(green leaf volatiles, GLVs)的形成特征、对植物的生态生理作用及调控机制等方面的最新研究进展。GLVs是指植物经十八碳烷酸途径过氧化物酶分支途径产生的含6个碳原子的醛、醇及其酯。除叶片外, 植物的根、茎、果实、种子等部位均可以合成该类化合物, 合成调控存在转录和转录后水平的调控。在特定植物中, GLVs合成及其组分还受到植物生长发育阶段和生长季节等外源环境的影响。昆虫啃食、微生物感染以及有益真菌的定植等生物逆境与缺氮等物理逆境均具有诱导GLVs合成的作用。除了参与植物特有气味形成外, GLVs在植物直接和间接防御应答中发挥着重要的作用。GLVs不仅具有抑制微生物和多种昆虫繁殖的作用, 还具有诱导多种防御化合物合成、预置(prime)有关信号途径的作用。基于GLVs在植物界的广泛存在性和在防御应答中的多层次作用, 该文作者提出了GLVs在道地药材品质形成中的潜在作用及开展相关研究的必要性和紧迫性。

关键词: 中药材, 防御应答, 绿叶挥发物, 诱导效应, 品质形成

Abstract:

We review advances in understanding the production, eco-physiological roles, and related regulatory mechanism of green leaf volatiles (GLVs). GLVs are six-carbon aldehydes, alcohols and esters derived from the hydroperoxide lyase branch of the octadecanoic acid pathway in plants. Besides leaves, other tissues such as root, stem, fruit, and seed are also reported to be capable of producing the GLVs regulated at the levels of transcription and post-transcription in plants. GLVs composition and amounts are influenced by the developmental stage and growth environment such as seasonal change in a given plant. In addition, the formation of GLVs can be induced by biotic stress such as insect attack, inoculation of pathogenic bacteria and inhabitation of beneficial fungi, as well as abiotic ones such as nitrogen deficiency. Although GLVs are primarily recognized as important contributors to unique flavors, they also play important roles in direct and indirect defense responses. They not only inhibit the reproduction of bacteria, fungi and insects, but also induce the synthesis of various defensive compounds. They can also prime different signal induction pathways. The potential roles that GLVs played in the quality formation of Chinese herbs, especially genuine ones, are discussed based on their wide distribution and multi-layered defensive roles in plants. It is necessary and urgent to carry out related research on GLVs in Chinese herbs for their sustainable and green development.

Key words: Chinese medical herbs, defense response, green leaf volatiles, induced effect, quality formation

孙海峰, 李震宇, 武滨, 秦雪梅. 绿叶挥发物产生特征及其生态生理作用研究进展. 植物生态学报, 2013, 37(3): 268-275. DOI: 10.3724/SP.J.1258.2013.00027

SUN Hai-Feng, LI Zhen-Yu, WU Bin, QIN Xue-Mei. Review of recent advances on the production and eco-physiological roles of green leaf volatiles. Chinese Journal of Plant Ecology, 2013, 37(3): 268-275. DOI: 10.3724/SP.J.1258.2013.00027

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2013.00027

https://www.plant-ecology.com/CN/Y2013/V37/I3/268

图/表 2

参考文献 49

[1]	Bate NJ, Rothstein SJ (1998). C₆-volatiles derived from the lipoxygenase pathway induce a subset of defense-related genes. The Plant Journal, 16, 561-569. DOI URL PMID
[2]	Beauchamp J, Wisthaler A, Hansel A, Kleist E, Miebach M, Ninemets U, Schurr U, Wildt J (2005). Ozone induced emissions of biogenic VOC from tobacco: relationships between ozone uptake and emission of LOX products. Plant, cell & Environment, 28, 1334-1343.
[3]	Boué SM, Shih BY, Carter-Wientjes CH, Clevelland TE (2003). Identification of volatile compounds in soybean at various developmental stages using solid phase microextraction. Journal of Agricultural and Food Chemistry, 51, 4873-4876. DOI URL PMID
[4]	Croft K, Juttner F, Slusarenko AJ (1993). Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv. Phaseolicola. Plant Physiology, 101, 13-24. URL PMID
[5]	Curtius T, Franzen H (1911). Aldehyde aus Grunen Pflanzenteilen, Winter Press, Heidelberg. 1142-1143. (in German)
[6]	Deng WL, Hamilton-Kemp TR, Nielsen MT, Andersen RA, Collins GB, Hildebrand DF (1993). Effects of six-carbon aldehydes and alcohols on bacterial proliferation. Journal of Agricultural and Food Chemistry, 41, 506-510.
[7]	Dickens JC (1989). Green leaf volatiles enhance aggregation pheromone of boll weevil,Anthonomus grandis. Entomologia Experimentalis et Applicata, 52, 191-203.
[8]	Engelberth J, Alborn HT, Schmelz EA, Tumlinson JH (2004). Airborne signals prime plants against insect herbivore attack. Proceedings of the National Academy of Sciences of the Unitel States of America, 101, 1781-1785.
[9]	Fontana A, Reichelt M, Hempel S, Gershenzon J, Unsicker SB (2009). The effects of arbuscular mycorrhizal fungi on direct and indirect defense metabolites of Plantago lanceolata L. Journal of Chemical Ecology, 35, 833-843. DOI URL PMID
[10]	Gardner HW (1979). Lipid hydroperoxide reactivity with proteins and amino acids: a review. Journal of Agricultural and Food Chemistry, 27, 220-229. DOI URL
[11]	Gardner HW, Dornbos DL, Desjardins AE (1990). Hexanal,trans-2-Hexenal, trans-2-nonenal inhibit soybean, Glycine max, seed germination. Journal of Agricultural and Food Chemistry, 38, 1316-1320.
[12]	Gomi K, Yamaski Y, Yamamoto H, Akimitsu K (2003). Characterization of a hydroperoxide lyase gene and effect of C₆-volatiles on expression of genes of the oxylipin metabolism in Citrus. Journal of Plant Physiology, 160, 1219-1231. URL PMID
[13]	Gueldner RC, Wilson DM, Heidt AR (1985). Volatile compounds inhibiting Aspergillus flavus. Journal of Agricultural and Food Chemistry, 33, 411-413.
[14]	Hamilton-Kemp TR, McCracken C, Loughrin JH, Andersen RA, Hildebrand DF (1992). Effects of some natural volatile compounds on the pathogenic fungiAlternaria alternate and Botrytis cinerea. Journal of Chemical Ecology, 18, 1083-1091. URL PMID
[15]	Hatanaka A (1993). The biogeneration of green odour by green leaves. Phytochemistry, 34, 1201-1218. DOI URL
[16]	Hatanaka A, Harada T (1973). Formation of cis-3-hexena1, trans-2-hexenal and cis-3-hexenol in macerated Thea sinensis leaves. Phytochemistry, 12, 2341-2346.
[17]	Hatanaka A, Kajiwara T, Sekiya J (1976). Seasonal variations in trans-2-hexenal and linolenic acid in homogenates of Thea sinensis leaves. Phytochemistry, 15, 1889-1891.
[18]	Hatanaka A, Kajiwara T, Sekiya J (1987). Biosynthetic pathway for C₆-aldehydes formation from linolenic acid in green leaves. Chemistry and Physics of Lipids, 44, 341-361.
[19]	Hildebrand DF, Brown GC, Jackson DM, Hamilton-Kemp TR (1993). Effects of some leaf-emitted volatile compounds on aphid population increase. Journal of Chemical Ecology, 19, 1876-1887.
[20]	Hirao T, Okazawa A, Harada K, Kobayashi A, Muranaka T, Hirata K (2012). Green leaf volatiles enhance methyl jasmonate response in Arabidopsis Journal of Bioscience and Bioengineering, 114, 540-545. DOI URL PMID
[21]	Hubert J, Münzbergova Z, Nesvorna M, Poltronieri P, Santino A (2008b). Acaricidal effects of natural six-carbon and nine-carbon aldehydes on stored-product mites. Experimental and Applied Acarology, 44, 315-321. DOI URL PMID
[22]	Hubert J, Münzbergova Z, Santino A (2008a). Plant volatile aldehydes as natural insecticides against stored-product beetles. Pest Management Science, 64, 57-64. DOI URL PMID
[23]	Hubert J, Nemcova M, Asapaly G, Stejskal V (2006). The toxicity of bean flour (Phaseolus vulgaris) to stored-product mites (Acari: Acaridida). Plant Protection Science, 42, 125-129.
[24]	Jardine K, Barron-Gafford GA, Norman JP, Abrell L, Monson RK, Meyers KT, Pavao-Zuckerman M, Dontsova K, Kleist E, Werner C, Huxman TE (2012). Green leaf volatiles and oxygenated metabolite emission bursts from mesquite branches following light-dark transitions. Photosynthesis Research, 113, 321-333. URL PMID
[25]	Kasai H, Maekawa M, Kawai K, Hachisuka K, Takahashi Y, Nakamura H, Sawa R, Matsui S, Matsuda T (2005). 4-oxo-2-hexenal, a mutagen formed by ω-3 fat peroxidation, causes DNA adduct formation in mouse organs. Industrial Health, 43, 699-701. URL PMID
[26]	Kishimoto K, Matsui K, Ozawa R, Takabayashi J (2005). Volatile C6-aldehydes and allo-ocimene activate defense genes and induce resistance against Botrytis cinerea in Arabidopsis thaliana. Plant Cell Physiology, 46, 1093-1102. DOI URL PMID
[27]	Kishimoto K, Matsui K, Ozawa R, Takabayashi J (2006). ETR-, JAR1- and PAD2-dependent signaling pathways are involved in C6-aldehyde-induced defense responses of Arabidopsis. Plant Science, 171, 415-423. DOI URL PMID
[28]	Kohlmann M, Bachmann A, Weichert H, Kolbe A, Balkenhohl T, Wasternack C, Feussner I (1999). Formation of lipoxygenase-pathway-derived aldehydes in barley leaves upon methyl jasmonate treatment. European Journal of Biochemistry, 260, 885-895. DOI URL PMID
[29]	Loreto F, Barta C, Brilli F, Noguest I (2006). On the induction of volatile organic compound emissions by plants as consequence of wounding or fluctuations of light and temperature. Plant, Cell & Environment, 29, 1820-1828. URL PMID
[30]	Matsui K (2006). Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism. Current Opinion in Plant Biology, 9, 274-280. DOI URL PMID
[31]	Matsui K, Sugimoto K, Mano J, Ozawa R, Takabayashi J (2012). Differential metabolisms of green leaf volatiles in injured and intact parts of a wounded leaf meet distinct ecophysiological requirements. PLoS One, 7, e36433. DOI URL PMID
[32]	Nakamura S, Hatanaka A (2002). Green-leaf-derived C₆-aroma compounds with potent antibacterial action that act on both Gram-negative and Gram-positive bacteria. Journal of Agricultural and Food Chemistry, 50, 7639-7644. URL PMID
[33]	Pattee HE, Singleton JA, Johns EB, Mullin BC (1970). Changes in the volatile profile of peanuts and their relation to enzyme activity levels during maturation. Journal of Agricultural and Food Chemistry, 18, 353-356.
[34]	Salas JJ, Sanchez C, Garcia-Gonzalez DL, Aparicio R (2005). Impact of the suppression of lipoxygenase and hydroperoxide lyase on the quality of the green odor in green leaves. Journal of Agricultural and Food Chemistry, 53, 1648-1655. DOI URL PMID
[35]	Schuman MC, Barthel K, Baldwin IT (2012). Herbivory- induced volatiles functions as defenses increasing fitness of the native plant Nicotiana attenuate in nature. eLife, 1:e00007. DOI URL PMID
[36]	Serradilla MJ, Martin A, Heranandez A, Lopez-Corrales M, Lozano M, Cordoba M (2010). Effects of the commercial ripening stage and postharvest storage on microbial and aroma changes of “Ambrunés” sweet cherries. Journal of Agricultural and Food Chemistry, 58, 9157-9163. DOI URL PMID
[37]	Shiojiri K, Ozawa R, Matsui K, Sabelis MW, Takabayashi J (2012). Intermittent exposure to traces of green leaf volatiles triggers a plant response. Scientific Reports, 2, 378. DOI URL PMID
[38]	Snoeren TAL, Kappers IF, Broekgaarden C, Mumm R, Dicke M, Bouwmeester HJ (2010). Natural variation in herbivore-induced volatiles in Arabidopsis thaliana. Journal of Experimental Botany, 61, 3041-3056. URL PMID
[39]	Sun HF, Xie DS, Guo XQ, Zhang LZ, Li ZY, Wu B, Qin XM (2010). Study on the relevance between beany flavor and main bioactive components in radix Astragali. Journal of Agricultural and Food Chemistry, 58, 5568-5573. DOI URL PMID
[40]	Vancanneyt G, Sanz C, Farmaki T, Paneque M, Ortego F, Castanera P, Sanchez-Serrano JJ (2001). Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance. Proceedings of the National Academy of Sciences of the United States of America, 98, 8139-8144. DOI URL PMID
[41]	von Mérey G, Veyrat N, Mahuku G, Valdez RL, Turlings TCJ, D’Alessandro M (2011). Dispensing synthetic green leaf volatiles in maize fields increases the release of sesquiterpenes by the plants, but has little effect on the attraction of pest and beneficial insects. Phytochemistry, 72, 1838-1847. DOI URL
[42]	Whitman DW, Eller FJ (1990). Parasitic wasps orient to green leaf volatiles. Chemoecology, 1, 69-75.
[43]	Xie DS, Wu B, Sun HF, Guo XQ, Zhang LZ, Qin XM (2009). Correlation between the flavor and quality of radix Astragali: the extraction and characterization of lipoxygenase in radix Astragali. World Science and Technology, 11, 375-381.
[44]	Xu HD, Zhou Y, Lei T (2011). Volatile analysis in fresh and dried Astragali radix. Food Science, 32, 171-174. (in Chinese with English abstract)
	[ 徐怀德, 周瑶, 雷霆 (2011). 鲜黄芪和干黄芪挥发性化学成分比较分析. 食品科学, 32, 171-174. ]
[45]	Xu YC, Barringer S (2009). Effect of temperature on lipid-related volatile production in tomato puree. Journal of Agricultural and Food Chemistry, 57, 9108-9113. DOI URL PMID
[46]	Yan ZG, Wang CZ (2006). Wound-induced green leaf volatiles cause the release of acetylated derivatives and a terpenoid in maize. Phytochemistry, 67, 34-42. DOI URL PMID
[47]	Yi HS, Yang JW, Ghim SY, Ryu CM (2011). A cry for help from leaf to root: Aboveground insect feeding leads to the recruitment of rhizosphere microbes for plant self- protection against diverse attacks. Plant Signaling & Behavior, 6, 1192-1194. URL PMID
[48]	Zeringue HJ (1992). Effects of C₆-C₁₀ alkenals and alkanals on ecliciting a defence response in the developing cotton boll. Phytochemistry, 31, 2305-2308. DOI URL
[49]	Zhuang H, Hamilton-Kemp TR, Andersen RA, Hildebrand DF (1992). Developmental change in C₆-aldehyde formation by soybean leaves. Plant Physiology, 100, 80-87. DOI URL PMID