白刺对不同浓度混合盐碱胁迫的生理响应

doi:10.3773/j.issn.1005-264x.2010.10.010

摘要/Abstract

摘要：

为研究白刺(Nitratia tangutorum)对盐碱生境的适应能力, 对二年生白刺实生苗进行了20组不同盐、碱浓度的胁迫处理。研究结果表明: 随着盐、碱浓度加大, 白刺叶片质膜所受伤害逐渐增加, 在300 mmol·L^-1盐浓度下, 白刺膜透性大幅度增加, 电解质大量外渗, 质膜的选择透过性遭到破坏; 丙二醛作为膜脂过氧化的产物, 它的变化规律与质膜透性的变化规律相一致, 两者呈极显著正相关(r = 0.796); 白刺叶片中的超氧化物歧化酶、过氧化物酶和过氧化氢酶活性随着盐胁迫浓度和pH的增加而有不同程度的升高, 增强了清除细胞内活性氧的能力, 对膜系统起到了一定的保护作用; 线性回归分析表明对白刺生长有重要影响的4个胁迫因素的重要性排序是盐浓度> pH > [HCO₃^-] > [CO₃^2-]。综合各项指标, 盐浓度在300 mmol·L^-1以下, pH小于10.59时, 白刺能够较好耐受, 具有较强的耐盐碱适应能力。

关键词: 混合盐碱胁迫, 质膜透性, 白刺, 保护酶活性

Abstract:

Aims Our objective was to investigate the saline-alkali tolerance mechanism of Nitratia tangutorum to provide the basis for its use in saline areas in Heilongjiang Province of China and contribute to the theoretical understanding of urban tree species.

Methods We treated seedlings of N. tangutorum to 20 kinds of mixed salt solutions with different salinities and alkalinities and examined saline-alkali stress on lipid peroxidation and enzyme activities.

Important findings Injury to the plasma membrane of leaves increased with increasing salinity and alkalinity. Cell membrane permeability and electrolyte leakage of leaves were enhanced under salinity of 300 mmol·L^-1. The selectivity of plasma membrane permeability was damaged. The variation of malondialdehyde lipid peroxidation as plasmalemma peroxide product was consistent with that of electrolyte leakage, which showed a significant positive correlation (r = 0.796). The activities of superoxide dismutase, peroxidase and catalase increased with both increased salinity and pH, which enhanced the ability of the active cell membrane system. Linear regression analysis showed that the significance order of four stress factors is salinity > pH > [HCO₃^-] > [CO₃^2-]. Nitratia tangutorum had a high tolerance to complex saline-alkali stress when the salinity was < 300 mmol·L^-1 and pH was < 10.59.

Key words: complex saline-alkali stress, membrane permeability, Nitratia tangutorum, protective enzyme activity

闫永庆, 刘兴亮, 王崑, 樊金萍, 石溪婵. 白刺对不同浓度混合盐碱胁迫的生理响应. 植物生态学报, 2010, 34(10): 1213-1219. DOI: 10.3773/j.issn.1005-264x.2010.10.010

YAN Yong-Qing, LIU Xing-Liang, WANG Kun, FAN Jin-Ping, SHI Xi-Chan. Effect of complex saline-alkali stress on physiological parameters of Nitratia tangutorum. Chinese Journal of Plant Ecology, 2010, 34(10): 1213-1219. DOI: 10.3773/j.issn.1005-264x.2010.10.010

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https://www.plant-ecology.com/CN/Y2010/V34/I10/1213

图/表 8

表1 各处理液的盐分组成摩尔比以及相应的pH值

Table 1 Salt composition, molar ratio and corresponding pH of solutions of each treament

处理组 Treatment	盐分组成及摩尔比 Salt composition and molar ratio				各盐浓度(mmol·L^-1)处理组对应的pH值 pH values corresponding to various salinity treatments (mmol·L^-1)
处理组 Treatment	NaCl	Na₂SO₄	NaHCO₃	Na₂CO₃	0	100	200	300	400
CK	0	0	0	0	6.87	-	-	-	-
A	1	1	0	0	-	7.39	7.47	7.54	7.73
B	1	0	1	0	-	8.13	8.24	8.31	8.46
C	1	1	1	1	-	9.47	9.56	9.87	9.98
D	0	1	0	1	-	10.42	10.57	10.62	10.75
E	0	0	1	1	-	11.18	11.35	11.47	11.73

图1 混合盐碱胁迫对白刺叶片电解质外渗率的影响。由于组内pH值的变化明显小于组间, 所以分析数据时以同组各浓度pH值的平均值作为7个盐组合的pH值。CK、A、B、C、D、E, 同表1。

Fig. 1 Effect of various complex saline-alkali stress on electrolyte leakage in leaves of Nitratia tangutorum. As the intraclass changes in pH, significantly less than that between groups, so analysis of the data to various concentrations of the same group as the pH value of the average of seven combinations of pH. CK, A, B, C, D, E, see Table 1.

图2 混合盐碱胁迫对白刺叶片丙二醛含量的影响。图注同图1。

Fig. 2 Effect of complex saline-alkali stress on malondialdehyde (MDA) content in leaves of Nitratia tangutorum. Notes see Fig. 1.

图3 混合盐碱胁迫对白刺叶片超氧化物歧化酶(SOD)活性的影响。图注同图1。

Fig. 3 Effect of complex saline-alkali stress on superoxide dismutase (SOD) activity in leaves of Nitratia tangutorum. Notes see Fig. 1.

图4 混合盐碱胁迫对白刺叶片过氧化物酶(POD)活性的影响。图注同图1。

Fig. 4 Effect of complex saline-alkali stress on peroxidase (POD) activity in leaves of Tanguticum nitratia. Notes see Fig. 1.

图5 混合盐碱胁迫对白刺叶片过氧化氢酶(CAT)活性的影响。图注同图1。

Fig. 5 Effect of complex saline-alkali stress on catalase (CAT) activity in leaves of Nitratia tangutorum. Notes see Fig. 1.

表2 盐度和pH及其交互作用对白刺幼苗不同胁变指标的影响

Table 2 Effect of salinity, pH and their interaction effects on different strain indexes of Nitratia tangutorum seedlings

胁变指标 Strain index	盐浓度 Salinity		pH值 pH value		盐浓度× pH Salinity× pH
胁变指标 Strain index	F	p	F	p	F	p
电解质外渗率 Electrolyte leakage	288.063	0.000^**	64.538	0.000^**	14.267	0.000^**
丙二醛含量 MDA content	4.071	0.000^**	3.821	0.070^**	0.796	0.053^*
SOD活性 SOD activity	73.532	0.000^**	4.862	0.023^*	2.908	0.318^NS
POD活性 POD activity	426.774	0.002^**	88.905	0.000^**	17.193	0.000^**
CAT活性 CAT activity	289.215	0.003^**	17.339	0.000^**	6.313	0.000^**

表3 胁变因素与各胁变指标的线性回归分析

Table 3 Partial regression coefficient and test of each variable

胁变指标 Strain index	pH		盐浓度(salinity)		CO₃^2-		HCO₃^-		模拟方程 Best-fitting equation
胁变指标 Strain index	t	p	t	p	t	p	t	p	模拟方程 Best-fitting equation
电解质外渗率 Electrolyte leakage rate	5.502	0.000^**	7.133	0.000^**	-2.827	0.012^*	-	-	Y = 6.996X₁+ 0.069X₂+ 0.793 - 0.084X₃- 3.67 R² = 0.934
丙二醛含量 MDA content	5.800	0.000^**	7.969	0.000^**	-	-	-	-	Y = 0.298X₁+ 0.005X₂- 1.090 R² = 0.930
SOD活性 SOD activity	-1.628	0.022^*	4.611	0.000^**	-	-	-	-	Y = -7.768X₁+ 0.284X₂+ 220.670 R²= 0.855
POD活性 POD activity	3.541	0.003^**	3.250	0.005^**	-3.102	0.007^**	-	-	Y = 980.800X₁+ 6.839X₂- 20.171X₃- 4078.915 R² = 0.846
CAT活性 POD activity	4.433	0.000^**	5.493	0.000^**	-2.142	0.048^*	-	-	Y = 9.875X₁+ 0.093X₂- 0.112X₃- 49.073 R² = 0.920

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