样品保存条件对土壤与植物全碳全氮含量的影响

doi:10.17521/cjpe.2016.0286

摘要/Abstract

摘要：

土壤与植物的保存样品对生态学研究有重要意义, 但目前未得到广泛应用。该研究的目的是比较样品保存前后全碳、全氮含量的变化, 探讨保存样品应用于长时间序列的生态学研究的可行性。研究选取2011年生长季采集的土壤与植物样品, 保存4年之后测试碳、氮质量分数, 与保存前的测试数据通过成对t检验与线性回归进行比较。氮质量分数在不同保存条件下, 保存前后的测试数据之间均为1:1的线性关系, 除低温保存、粒径<2 mm的土壤样品相关系数为0.91外, 其余保存条件下相关系数均大于0.98。碳质量分数保存后的变化与保存条件有关, 粒径<0.15 mm的土壤与植物样品, 两次测试数据之间为1:1的线性关系, 相关系数均大于0.98; 粒径<2 mm的土壤与植物样品两次测试数据的线性关系斜率分别为1.26与1.04, 即2016年测试数据显著高于2012年。结果表明不同保存温度与样品粒径下, 样品保存后的氮含量没有变化; 而碳含量的稳定与样品粒径有关, 常温保存与<20 ℃低温下保存则无明显区别。建议用于碳氮元素分析的样品应充分干燥、研磨成<0.15 mm粒径后在常温环境下密封保存。

关键词: 碳质量分数, 氮质量分数, 样品粒径, 保存时间, 长时间序列

Abstract:

Aims The storage of soil and plant samples has important significance for ecological studies, but has not been widely used. This study aims to compare total carbon/nitrogen content of soil and plant samples before and after long term storage, and further to investigate the feasibility of archiving samples for time series ecological studies at large temporal scales.Methods Soil and plant samples were collected in the growing season in 2011. Carbon/nitrogen mass fraction were analyzed after four years of storage, and were compared with the data obtained before storage using pairwise t-test and linear regression.Important findings Nitrogen mass fractions of stored samples were linearly correlated to the data before storage along the 1:1 line under different storage conditions, and the correlation coefficient r was greater than 0.98 (except for soil samples stored at temperature lower than 20 °C and with particle size <2 mm, r = 0.91). The carbon mass fraction after storage was changed by the storage conditions. Carbon mass fractions of stored samples with particle size <0.15 mm were linearly correlated to the data before storage along the 1:1 line (r > 0.98). Carbon mass fractions of samples with particle size <2 mm increased after storage, and the slope of the linear relationship was 1.26 and 1.04 for soil and plant samples respectively. These results indicated that, nitrogen content of stored samples was stable under different storage conditions, while the stability of carbon content was affected by sample particle size but by storage temperature. Archived samples used for carbon/nitrogen analysis were suggested to be ground to particle size <0.15 mm under fully dry and completely sealed conditions.

Key words: carbon mass fraction, nitrogen mass fraction, sample particle size, storage time, long time series

陈雅涵, 谢宗强. 样品保存条件对土壤与植物全碳全氮含量的影响. 植物生态学报, 2017, 41(6): 632-638. DOI: 10.17521/cjpe.2016.0286

Ya-Han CHEN, Zong-Qiang XIE. Effects of storage conditions on total carbon and nitrogen contents of soil and plant samples. Chinese Journal of Plant Ecology, 2017, 41(6): 632-638. DOI: 10.17521/cjpe.2016.0286

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2016.0286

https://www.plant-ecology.com/CN/Y2017/V41/I6/632

图/表 4

表1 2012和2016年测定的土壤样品碳氮质量分数(平均值±标准偏差)

Table 1 Carbon and nitrogen mass fractions of soil samples analyzed in 2012 and 2016 (mean ± SD)

	粒径<0.15 mm Particle size <0.15 mm		粒径<2 mm Particle size <2 mm 低温 Low temperature
	低温 Low temperature	常温 Room temperature	粒径<2 mm Particle size <2 mm 低温 Low temperature
碳质量分数 Carbon mass fraction (%)
2012	1.56 ± 2.23	0.806 ± 0.634	1.54 ± 0.65
2016	1.53 ± 2.09	0.826 ± 0.633	1.69 ± 0.88
两次测试差异 Difference between the two times	-0.04^*	0.02^*	0.15^*
氮质量分数 Nitrogen mass fraction (%)
2012	0.129 ± 0.154	0.072 ± 0.036	0.128 ± 0.076
2016	0.129 ± 0.157	0.075 ± 0.037	0.138 ± 0.087
两次测试差异 Difference between the two times	ns	0.003^*	0.01^*

图1 2012和2016年测定的土壤样品碳氮质量分数的线性回归。A, B, 低温保存(<20 ℃), 粒径<0.15 mm。C, D, 常温保存, 粒径<0.15 mm。E, F, 低温保存(<20 ℃), 粒径<2 mm。C%, 碳质量分数; N%, 氮质量分数; r, 相关系数; s, 线性回归的斜率; 实线, 回归线; 虚线, 1:1线。

Fig. 1 Linear regression relationship between carbon/nitrogen mass fractions of soil samples analyzed in 2012 and 2016 under different storage conditions. A, B, low temperature (<20 °C), particle size <0.15 mm. C, D, room temperature, particle size <0.15 mm. E, F, low temperature (<20 °C), particle size <2 mm. C%, carbon mass fraction; N%, nitrogen mass fraction; r, correlation coefficient; s, slope of linear regression; solid line, regression line; dashed line, 1:1 line.

表2 2012和2016年测定的植物样品碳氮质量分数(平均值±标准偏差)

Table 2 Carbon and nitrogen mass fractions of plant samples analyzed in 2012 and 2016 (mean ± SD)

	低温 Low temperature 粒径<2 mm Particle size <2 mm	常温 Room temperature 粒径<0.15 mm Particle size <0.15 mm
碳质量分数 Carbon mass fraction (%)
2012	44.30 ± 4.40	44.30 ± 4.40
2016	46.09 ± 4.21	44.89 ± 4.42
两次测试差异 Difference between the two times	1.79^*	0.58^*
氮质量分数 Nitrogen mass fraction (%)
2012	0.789 ± 0.620	0.789 ± 0.620
2016	0.796 ± 0.622	0.774 ± 0.619
两次测试差异 Difference between the two times	ns	-0.01^*

图2 2012和2016年测定的植物样品碳氮质量分数的线性回归。A, B, 低温保存(<20 ℃), 粒径<2 mm。C, D, 常温保存, 粒径<0.15 mm。C%, 碳质量分数; N%, 氮质量分数; r, 相关系数; s, 线性回归的斜率; 实线, 回归线; 虚线, 1:1线。

Fig. 2 Linear regression relationship between carbon/nitrogen mass fraction of plant samples analyzed in 2012 and 2016 under different storage conditions. A, B, low temperature (<20 °C), particle size <2 mm. C, D, room temperature, particle size <0.15 mm. C%, carbon mass fraction; N%, nitrogen mass fraction; r, correlation coefficient; s, slope of linear regression; solid line, regression line; dashed line, 1:1 line.

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