收稿日期: 2007-11-01
录用日期: 2007-12-10
网络出版日期: 2009-01-30
基金资助
广东省自然科学基金(06300472);中山大学青年教师科研启动基金和中山大学张宏达科学研究基金(ZHD200402)
PHYSIOLOGICAL RESPONSES OF THREE BRYOPHYTE SPECIES OF SOUTH CHINA TO SIMULATED NITROGEN DEPOSITION
Received date: 2007-11-01
Accepted date: 2007-12-10
Online published: 2009-01-30
该文研究了华南地区常见的3种苔藓植物大灰藓(Hypnum plumaeforme)、刺边小金发藓拟刺亚种(Pogonatum cirratum subsp. fuscatum)和石地钱(Reboulia hemisphaerica)在模拟N沉降条件下碳氮代谢的响应特征, 探讨了N沉降对这3种苔藓植物生长的影响。结果表明: 3种植物对N沉降的响应存在差异。大灰藓在加氮浓度为0 ~ 60 kg N·hm-2的范围内, 光合速率、淀粉、可溶性糖、总氮及可溶性蛋白的含量均随加氮浓度的升高而上升; 对照条件下NR(硝酸还原酶)可诱导活性很高, 表明其对氮的需求强烈; 膜K+渗漏在加氮浓度为20 kg N·hm-2时高于对照, 但更高的加氮浓度下并未使其进一步上升。刺边小金发藓拟刺亚种在加氮浓度为0~40 kg N·hm-2的范围内, 碳氮代谢的多数指标的变化趋势与大灰藓相似, 但当N沉降高于40 kg N·hm-2时, 呈现相反的变化趋势; 在对照条件下具一定的NR可诱导活性, 但人工加氮使其可诱导活性急剧下降。石地钱的可溶性糖及可溶性蛋白含量在不同处理条件下变化不明显, 但光合速率和淀粉含量在加氮浓度为20和40 kg N·hm-2时低于对照及加氮浓度为60 kg N·hm-2时, 而植物总氮含量的变化趋势刚好相反; NR固有活性及可诱导活性在各种N处理条件下均极低, 显示其对NO3-利用率低。刺边小金发藓拟刺亚种和石地钱的膜K+渗漏仅在60 kg N·hm-2处理时显著上升。
刘滨扬, 刘蔚秋, 雷纯义, 张以顺 . 三种苔藓植物对模拟N沉降的生理响应[J]. 植物生态学报, 2009 , 33(1) : 141 -149 . DOI: 10.3773/j.issn.1005-264x.2009.01.016
Aims Increasing global N deposition will lead to severe damage to bryophytes due to their sensitivity to N deposition. Our objective is to examine the responses of three species of bryophytes, widely distributed in South China, to simulated N deposition.
Methods Four N addition treatments (Control, 20, 40 and 60 kg N·hm-2) in three replicates were established for Reboulia hemisphaerica, Hypnum plumaeforme, and Pogonatum cirratum subsp. fuscatum. The N additions were divided into four applications.
Important findings With N addition treatments in the range of 0-60 kg N·hm -2 for H. plumaeforme, photosynthesis, concentrations of starch, soluble sugars, total N and soluble proteins all increased with increasing N addition. Inducible nitrate reductase activity (NRA) of H. plumaeforme at control conditions was very high, indicating its N-limited situation. K + leakage of H. plumaeforme increased at 20 kg N·hm -2 treatment, but did not further increase at higher N addition concentrations. Most of the physiological indices of P. cirratum subsp. fuscatum showed similar trends within an N treatment of 0-40 kg N·hm -2, but showed opposite trends at >40 kg N·hm -2. Inducible NRA of P. cirratum subsp. fuscatum at the control condition was 2.20 µg N·g -1 FW·h-1, but decreased dramatically when treated with N. Its K+ leakage only increased significantly at the 60 kg N·hm-2 N addition treatment. Soluble sugars and soluble proteins of R. hemisphaerica were not significantly different at different N treatments, but photosynthesis and the starch concentration were lower at 20 and 40 kg N·hm -2treatments than at control and 60 kg N·hm-2 treatments, while the total N concentration changed inversely. The inducible and constituted NRA of R. hemisphaerica were both very low for all treatments, indicating its restricted ability to utilize nitrates. K + leakage of R. hemisphaerica increased significantly only at an N addition of 60 kg N·hm -2.
Key words： nitrogen deposition; bryophytes; physiological response
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