两种生境条件下6种牧草叶绿素含量及荧光参数的比较
收稿日期: 2010-12-20
录用日期: 2011-04-22
网络出版日期: 2011-06-30
Comparison of chlorophyll content and fluorescence parameters of six pasture species in two habitats in China
Received date: 2010-12-20
Accepted date: 2011-04-22
Online published: 2011-06-30
昆仑山前山牧场海拔较高, 策勒绿洲海拔相对较低, 两者生境差异较大。以昆仑山前山牧场和策勒绿洲边缘两种不同生境条件下生长的6种牧草: 冰草(Agropyron cristatum)、无芒雀麦(Bromus inermis)、矮生高羊茅(Festuca elata)、披碱草(Elymus dahuricus )、红豆草(Onobrychis pulchella)及和田大叶(Medicago sativa var. luxurians)为试验材料, 研究了不同生境条件下牧草叶片叶绿素含量及叶绿素荧光动力学参数的变化情况。结果显示: (1)在两种生境条件下, 昆仑山前山牧场生境生长的牧草叶绿素a、叶绿素b、总叶绿素的含量明显较高, 生长在策勒绿洲生境的牧草品种叶绿素a/b值较高; (2)昆仑山前山牧场生境牧草最大荧光、光系统II (PSII)最大光化学效率、PSII潜在活性和单位面积反应中心的数量的值明显高于策勒绿洲生境品种, 而初始荧光、单位反应中心吸收的光能、单位反应中心捕获的能量、单位反应中心耗散的能量、荧光诱导曲线初始斜率值则低于策勒绿洲生境品种。因此, 两种生境下环境因子发生了改变, 对牧草产生综合的胁迫作用; 策勒绿洲生境明显对牧草生长产生了抑制, 策勒绿洲生境牧草的色素含量降低以及PSII的机构遭到损坏, 导致反应中心一部分失活或裂解, 剩余有活性的反应中心的效率增加, 昆仑山生境则相对比较适宜牧草生长; 两种生境不同牧草叶绿素含量和叶绿素荧光参数的变化幅度不同。
李磊, 李向义, 林丽莎, 王迎菊, 薛伟 . 两种生境条件下6种牧草叶绿素含量及荧光参数的比较[J]. 植物生态学报, 2011 , 35(6) : 672 -680 . DOI: 10.3724/SP.J.1258.2011.00672
Aims Qianshan Pasture in the Kunlun Mountains and Cele Oasis of China are different habitats with different elevations. We compared six pasture species in terms of chlorophyll (Chl) content and fluorescence parameters to investigate the effect of habitat on photosystem II (PSII).
Methods Agropyron cristatum, Bromus inermis, Festuca elata, Elymus dahuricus, Onobrychis pulchella and Medicago sativa var. luxurians, were grown in both sites. Leaf pigment content was measured by 95% ethanol method, and PSII chlorophyll fluorescence parameters were monitored by using a Plant Efficiency Analyzer (PEA).
Important findings The measured physiological parameters differed greatly in all six species growing in the two habitats. In Qianshan Pasture, all species showed obviously higher Chl a, Chl b and total Chl content, while those growing in Cele Oasis had higher Chl a/Chl b. Plants in Kunlun Mountains also had markedly higher maximum fluorescence yield (Fm), maximum photochemical efficiency of PSII (Fv/Fm), potential activity of PSII (Fv/Fo) and active reaction centers per cross-section (RC/CSo), as well as lower minimum fluorescence yield (Fo), absorption flux per reaction center (ABC/RC), maximal trapping flux per reaction center (TRo/RC), flux of dissipated excitation energy per reaction center (DIo/RC) and initial slope of fluorescence intensity (Mo) than those in the Cele Oasis. These parameters also showed large differences in variation amplitude between habitats. The results indicated that environmental conditions in the Cele Oasis were stressful to pasture species. It decreased chlorophyll content and inactivated PSII. The habitat in Kunlun Mountains was relatively favorable for these species.
Key words： chlorophyll; chlorophyll fluorescence; habitat; pasture
| [1] | Anderson JM, Aro EM (1994). Grana stacking and protection of photosystem II in thylakoid membranes of higher plant leaves under sustained high irradiance: an hypothesis. Photosynthesis Research, 41, 315-326. |
| [2] | Baker NR (1991). A possible role for photosystem II in environmental perturbations of photosynthesis. Physiologia Plantarum, 81, 563-570. |
| [3] | Cao Y (曹昀), Wang GX (王国祥), Zhang D (张聃) (2008). Effects of drought stress on the growth and chlorophyll fluorescence of reed seedling. Arid Land Geography (干旱区地理), 31, 862-869. (in Chinese with English abstract) |
| [4] | Gao Y (高玉), Gao ZK (高志奎), Zhang XH (张晓慧), Gao RF (高荣孚) (2009). Heat shock stress on photosystem II in white cucumbers probed by the fast fluorescence rise OJIP. Acta Ecologica Sinica (生态学报), 29, 3335-3341. (in Chinese with English abstract) |
| [5] | Han RH (韩瑞宏), Lu XS (卢欣石), Gao GJ (高桂娟), Yang XJ (杨秀娟) (2007). Photosynthetic physiological response of alfalfa (Medicago sativa) to drought stress. Acta Ecologica Sinica (生态学报), 27, 5229-5237. (in Chinese with English abstract) |
| [6] | Havaux M (1996). Short-term responses of photosystem I to heat stress: induction of a PSII-independent electron transport through PSI fed by stromal components. Photosynthesis Research, 47, 85-97. |
| [7] | Jiang CD, Gao HY, Zou Q (2003). Changes of donor and accepter side in photosystem 2 complex induced by iron deficiency in attached soybean and maize leaves. Photosynthetia, 41, 267-271. |
| [8] | Larcher W (1994). Hochgebirge: an den grenzen des wachs- tums. In: Morawetz W ed. ?kologische Grundwerte in ?sterreich Biosystematics and Ecology Series. Osterr Akad Wiss, Wien. 304-343. |
| [9] | Li HS (李合生) (2000). Plant Physiological Biochemical Experiment Principles and Techniques (植物生理生化试验原理和技术). Higher Education Press, Beijing. 137. (in Chinese) |
| [10] | Li PM (李鹏民), Gao HY (高辉远), Strasser RJ (2005). Application of the fast chlorophyll fluorescence induction dynamics in photosynthesis study. Journal of Plant Physiology and Molecular Biology (植物生理与分子生物学学报), 31, 559-566. (in Chinese with English abstract) |
| [11] | Li W (李伟), Cao KF (曹坤芳) (2006). Effects of drought stress on photosynthetic characteristics and chlorophyll fluorescence parameters in seedling of Terminthia paniculata grown under different light level. Acta Botanica Boreal-Occidentalia Sinica (西北植物学报), 26, 266-275. (in Chinese with English abstract) |
| [12] | Li YH (李彦慧), Meng QR (孟庆瑞), Li XY (李向应), Li BG (李保国), Yang JM (杨建民) (2008). Contents of photosynthetic pigment and chlorophyll fluorescence parameters in Prunus cerasifera var. atropurea leaves under SO2 stress. Acta Scientiae Circumstantiae (环境科学学报), 28, 2236-2242. (in Chinese with English abstract) |
| [13] | Qin JQ (秦建桥), Xia BC (夏北成), Zhao P (赵鹏) (2010). Photosynthetic physiological response of two Miscanthus floridulus populations to Cd stress. Acta Ecologica Sinica (生态学报), 30, 288-299. (in Chinese with English abstract) |
| [14] | Schreiber U, Berry JA (1977). Heat-induced changes in chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus. Planta, 136, 233-238. |
| [15] | Shi SB (师生波), Ben GY (贲桂英), Han F (韩发) (1999). Analysis of the solar UV-B radiation and plant UV-B- absorbing compounds in different regions. Acta Phytoecologica Sinica (植物生态学报), 23, 529-535. (in Chinese with English abstract) |
| [16] | Song CY (宋春雨), Liu XB (刘晓冰), Jin CX (金彩霞) (2002). Mechanisms of damage and acclimation of photosynthetic apparatus due to heat stress. System Sciences and Comprehensive Studies in Agriculture (农业系统科学与综合研究), 18, 252-256. (in Chinese with English abstract) |
| [17] | Srivastava A, Guissé B, Greppin H, Strasser RJ (1997). Regulation of antenna structure and electron transport in photosystem II of Pisum sativum under elevated temperature probed by the fast polyphasic chlorophyll a fluorescence transient: OKJIP. Biochimica et Biophysica Acta (BBA)- Bioenergetics, 1320, 95-106. |
| [18] | Srivastava A, Strasser RJ (1996). Stress and stress management of land plants during a regular day. Journal of Plant Physiology, 148, 445-455. |
| [19] | Strasser RJ, Srivastava A, Tsimilli-Michael M (2000). The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P eds. Photosynthesis: Mechanisms, Regulation and Adaptation. Taylor and Francis Press, London. 445-483. |
| [20] | Strasser RJ, Tsimill-Michael M, Srivastava A (2004). Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G, Govindjee eds. Advances in Photosynthesis and Respiration. KAP Press, Dordrecht, The Netherlands. 1-47. |
| [21] | Sundby C, Melis A, M?enp?? P, Andersson B (1986). Temperature-dependent changes in the antenna size of photosystem II. Reversible conversion of photosystem IIα to photosystem IIβ. Biochimica et Biophysica Acta (BBA)- Bioenergetics, 851, 475-483. |
| [22] | Sun XZ (孙宪芝), Zheng CS (郑成淑), Wang XF (王秀峰) (2008). Effects of high temperature stress on photosynthesis and chlorophyll fluorescence of cut flower chrysanthemum (Dendranthema grandiflora ‘Jinba’). Chinese Journal of Applied Ecology (应用生态学报), 19, 2149-2154. (in Chinese with English abstract) |
| [23] | Wang M (王梅), Gao ZK (高志奎), Huang RH (黄瑞虹), Wang HY (王惠英), Zhang WL (张文丽), Gao RF (高荣孚) (2007). Heat stress characteristics of photosystem II in eggplant. Chinese Journal of Applied Ecology (应用生态学报), 18, 63-68. (in Chinese with English abstract) |
| [24] | Wang WJ (王文杰), Li WX (李文馨), Zu YG (祖元刚), Wang XP (王晓鹏), He HS (贺海升) (2009). Differences in pigments and chlorophyll fluorescence parameters between stems and leaves of Eupatorium adenophorum under different temperature treatments. Acta Ecologica Sinica (生态学报), 29, 5424-5433. (in Chinese with English abstract) |
| [25] | Wen GS (温国胜), Tian HT (田海涛), Zhang MR (张明如), Jiang WW (蒋文伟) (2006). Application of chlorophyll fluorescence analysis in forest tree cultivation. Chinese Journal of Applied Ecology (应用生态学报), 17, 1973-1977. (in Chinese with English abstract) |
| [26] | Wu HY (吴韩英), Shou SY (寿森炎), Zhu ZJ (朱祝军), Yang XT (杨信廷) (2001). Effects of high temperature stress on photosynthesis and chlorophyll fluorescence in sweet pepper (Capsicum fructescens L.). Acta Horticulturate Sinica (园艺学报), 28, 517-521. (in Chinese with English abstract) |
| [27] | Zhu JT (朱军涛), Li XY (李向义), Zhang XM (张希明), Zeng FJ (曾凡江), Lin LS (林丽莎), Yang SG (杨尚功), Gui DW (桂东伟), Wang H (王辉), Liu B (刘波) (2010). Ecophysiological response of Calligonum roborovskii to the habitats in different altitudes in north slope of Kunlun Mountain. Acta Ecologica Sinica (生态学报), 30, 602-609. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |
