丛枝菌根菌丝桥介导刺槐-魔芋间碳转运和磷吸收
收稿日期: 2022-05-09
录用日期: 2022-10-10
网络出版日期: 2022-10-10
基金资助
国家自然科学基金(31901468);陕西省重点研发计划(2021NY-048);陕西省创新人才推进计划(2020KJXX-003)
Carbon transport and phosphorus uptake in an intercropping system of Robinia pseudoacacia and Amorphophallus konjac mediated by arbuscular mycorrhizal hyphal networks
Received date: 2022-05-09
Accepted date: 2022-10-10
Online published: 2022-10-10
Supported by
National Natural Science Foundation of China(31901468);Key Research and Development Program of Shaanxi Province(2021NY-048);Innovation Capacity Support Plan of Shaanxi Province(2020KJXX-003)
明确间作和接种丛枝菌根真菌(AMF)对刺槐(Robinia pseudoacacia)与魔芋(Amorphophallus konjac)碳转运和磷吸收的影响, 可为揭示间作刺槐对魔芋的防病促生机制和推广刺槐林下魔芋绿色高产栽培技术提供科学依据。该研究使用孔径25 μm尼龙网设置两室根箱隔网系统, 分为A室(刺槐不接种/接种AMF)和B室(单作刺槐/刺槐间作魔芋); 采用13C稳定性同位素技术对A室刺槐叶片进行标记, 研究其同化的碳与B室魔芋之间的传递, 以及AMF定植对两种作物的农艺性状、13C丰度和磷含量的影响。结果表明: (1)在接种条件下, 菌丝桥对B室刺槐和魔芋的侵染率分别达到47.1%和60.4%; 其中, 刺槐侵染率与单作直接接种处理相比降低14.1%。在间作体系中, 接种AMF提高了魔芋的生物量, 其地上干质量和地下根系干质量分别较未接种处理显著增加9.7%和36.2%。(2)与单作未接种处理相比, 间作未接种、单作接种和间作接种的刺槐同化的碳被更多地分配到根系和根际土壤(A室), 并以根系分泌物的形式穿越尼龙网到达邻近作物根际(B室)。(3)与各自未接种处理相比, 单作或间作接种显著提高B室刺槐和魔芋叶片、茎/叶柄、根系和全株磷含量。综上, AMF定植促进了刺槐向魔芋根际土和植株体内的碳转运, 且间作栽培可促进AMF的定植和宿主作物对磷的吸收。
何斐, 李川, Faisal SHAH, 卢谢敏, 王莹, 王梦, 阮佳, 魏梦琳, 马星光, 王卓, 姜浩 . 丛枝菌根菌丝桥介导刺槐-魔芋间碳转运和磷吸收[J]. 植物生态学报, 2023 , 47(6) : 782 -791 . DOI: 10.17521/cjpe.2022.0185
Aims The aim of this research was to clarify the effects of intercropping and inoculation with arbuscular mycorrhizal fungi (AMF) on carbon transport and phosphorus uptake in black locust (Robinia pseudoacacia) and konjac (Amorphophallus konjac). The results could provide empirical evidence to reveal the mechanisms of black locust intercropping for disease control and plant growth promotion of konjac, and popularize the green and high-yielding cultivation technique of konjac under black locust.
Methods The experiment was carried out in two-compartment rhizoboxes separated by a 25-μm nylon net, each of which comprised compartment A (non-inoculated or AMF-inoculated black locust) and compartment B (monocropped black locust or intercropped konjac). A 13C stable isotope labeling technique was used to label the leaves of black locust in compartment A with 13CO2. Carbon transport from black locust to konjac and the effects of AMF colonization on agronomic traits, 13C abundance, and phosphorus content in both crops were investigated.
Important findings The result showed that: (1) After inoculation, the AMF infection rate of black locust and konjac plants by hyphal links in compartment B reached 47.1% and 60.4%, respectively. For black locust, this AMF infection rate was 14.1% lower than that of directly inoculated plants under monocropping. In the case of intercropping, the biomass (dry mass) of AMF-inoculated konjac plants was 9.7% (aboveground parts) and 36.2% (belowground roots) higher than that of non-inoculated plants. (2) Compared with the non-inoculated plants under monocropping, the carbon fixed by photosynthesis of black locust plants in other treatments (non-inoculated + intercropping, inoculated + monocropping, and inoculated + intercropping) was mainly allocated to the plant roots and rhizosphere soil in compartment A, and more carbon passed through the nylon net in the form of root exudates to reach the rhizosphere of neighboring crop plants. (3) Compared with the respective non-inoculated controls, AMF inoculation in the monocropping and intercropping systems prominently improved phosphorus contents in the leaves, stems/petioles, roots, and total plants of black locust and konjac in compartment B. The findings suggest that AMF colonization could facilitate carbon transport from black locust to the rhizosphere soil and plant tissues of konjac. Intercropping konjac with black locust is an effective practice to promote AMF colonization and phosphorus uptake by both host plants.
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