太白山不同海拔森林根际土壤微生物碳利用效率差异性及其影响因素
收稿日期: 2022-03-09
录用日期: 2022-07-06
网络出版日期: 2022-10-11
基金资助
青海省2021年度第一批中央引导地方科技发展专项(2021ZY002);教育部“春晖计划”合作科研项目
Differences and influencing factors of microbial carbon use efficiency in forest rhizosphere soils at different altitudes in Taibai Mountain, China
Received date: 2022-03-09
Accepted date: 2022-07-06
Online published: 2022-10-11
Supported by
2021 First Funds for Central Government to Guide Local Science and Technology Development in Qinghai Province(2021ZY002);Ministry of Education “Chunhui Plan” Cooperative Scientific Research Project
探索森林根际土壤微生物碳利用效率(CUE)是权衡森林生态系统微生物合成代谢和分解代谢强弱的关键过程。然而不同海拔森林根际土壤微生物CUE的变化规律与影响因子尚不清楚。该研究选取秦岭太白山6个不同海拔的森林根际土壤为研究对象, 测定其理化性质、胞外酶活性、微生物群落与植被特征等指标, 利用酶化学计量比计算微生物CUE, 分析根际土壤微生物CUE沿海拔梯度的变化规律, 定量研究其影响因子。结果表明: 根际土壤微生物CUE随海拔升高总体呈上升趋势。CUE从最低海拔的0.505至最高海拔的0.527升高了4.36%, 但在海拔1 603和2 405 m处出现了下降。海拔梯度内根际土壤微生物CUE变化受多种环境因子综合影响, 土壤基质的影响(如可溶性有机碳和铵态氮含量)占主导地位, 植被因子次之, 二者分别解释了CUE变化的17.0%和5.7%, 且二者相互作用解释了CUE变化的31.9%。研究结果可为秦岭森林土壤微生物碳同化能力和固碳潜力, 以及全球变化背景下森林土壤碳循环提供科学依据。
张尧, 陈岚, 王洁莹, 李益, 王俊, 郭垚鑫, 任成杰, 白红英, 孙昊田, 赵发珠 . 太白山不同海拔森林根际土壤微生物碳利用效率差异性及其影响因素[J]. 植物生态学报, 2023 , 47(2) : 275 -288 . DOI: 10.17521/cjpe.2022.0090
Aims Under the background of changing carbon cycle process in forest ecosystems caused by global environmental change, the microbial carbon use efficiency (CUE) in forest rhizosphere soil is critical to determine the strength of microbial anabolism and catabolism in forest ecosystems. However, the variation and influencing factors of microbial CUE in rhizosphere soils at different altitudes remain undetermined.
Methods Rhizosphere soil at six different altitudes spanning four forest belts in Taibai Mountain was sampled to determine the physical and chemical properties, extracellular enzyme activity, and characteristics of microbial community and vegetation. Based on the stoichiometric ratio, the soil microbial CUE was estimated. Furthermore, the variation in microbial CUE of rhizosphere soil along the altitude gradient was analyzed to quantify the influencing factors of microbial CUE.
Important findings The results showed that the microbial CUE of rhizosphere soil exhibited an overall upward trend with the increase in altitude. The microbial CUE increased by 4.36% from 0.505 at the lowest altitude to 0.527 at the highest altitude, but decreased at 1 603 and 2 405 m. Based on the Mantel analysis, we identified four categories of factors (i.e., altitude, soil matrix, vegetation and microbe) that related to microbial CUE in rhizosphere soil. The variations of microbial CUE in rhizosphere soil are affected by multiple environmental factors, with the dominant factor being soil matrix (such as dissolved organic carbon (DOC) content, ammonium nitrogen (NH+4-N) content), followed by vegetation. Furthermore, the altitude factor and the microbial factor explained 2.6% and 3.1% of the CUE change, respectively. Although the microbial factors exerted no significant impact on microbial CUE, soil matrix, vegetation and microbe jointly explained 47.0% of the microbial CUE change. The variance partitioning analysis (VPA) quantitatively revealed the contribution of environmental factors to the change of microbial CUE, where soil matrix and vegetation explained 17.0% and 5.7% of the variation, respectively. While the interaction between soil matrix and vegetation accounted for 31.9% of the changes in microbial CUE. The above results indicated that the high-altitude rhizosphere soil in Taibai Mountain has a high carbon sequestration potential, and the carbon sequestration of forest rhizosphere soil may decrease with the intensification of global warming. The vertical temperature difference and the vertical differentiation of the vegetation belt induced by altitude gradient will alter the growth and metabolism environment of microorganisms in the rhizosphere soil. The comprehensive effect of multiple environmental factors dominated by soil matrix impacts the CUE of soil microorganisms, and ultimately changes the assimilation and catabolism processes of soil carbon. The results of this study can provide a scientific basis for the carbon assimilation capacity and carbon sequestration potential of forest soil microorganisms in Qinling Mountains, as well as the forest soil carbon cycle under the background of global change.
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