Abstract: Wheat plants (Triticum aestivum L. ) was grown in pots at three water levels (80%, 60% and 40% field water capacity) and three CO2 concentrations (350, 550 and 700 parts per million by volume (μmol·mol-1)) in open-top chambers (OTC). Leaf stomata characteristics were observed and measured. Drought and CO2 enrichment both influenced not only the leaf stomatal densities but also their distribution. Drought increased the abaxial stomatal densities while high CO2 decreased these densities. The variations of the stomatal distributions were diminished under drought and high CO2 treatments. Different temperatures also influenced the stomatal densities and distributions under constant water levels and CO2 concentrations.