Effects of Pb stress on C, N and P accumulation and allocation in Populus deltoids × P. nigra seedlings

doi:10.3724/SP.J.1258.2012.00578

Abstract

Abstract:

Aims Little information has been available on the effects of soil Pb pollution on plant nutrition. Our objective was to characterize the responses of poplar (Populus deltoids × P. nigra) in C, N and P accumulation and N and P use efficiency under different Pb stresses in acidic purple soil and alkaline purple soil.
Methods A controlled pot experiment using one-year old poplar was arranged with different treatments of four Pb concentrations (CK: 0, T1: 200, T2: 450 and T3: 2000 mg·kg^-1) in the field. Concentrations of C, N and P in plant components were measured over one growing season. We calculated C, N and P accumulation and N and P use efficiency.
Important findings Accumulations of C, N and P in poplar declined with increased Pb supplies in both acidic purple soil and alkaline purple soil. Accumulations of C, N and P in alkaline purple soil were larger than in acidic purple soil. Distribution patterns of C, N and P in poplar were altered in both soils when supplied with different Pb concentrations. C, N and P accumulation in fine roots significantly increased with increased Pb supplies in acidic purple soil, but decreased in fallen leaves. In contrast, C, N and P accumulation in fine roots decreased with increased Pb supplies in alkaline purple soil, but increased in fallen leaves. In addition, P use efficiency of the poplar in both soils increased with increased Pb supplies, but the N use efficiency significantly decreased under treatments T2 and T3. Results indicated that the accumulation of C, N and P in poplar had been inhibited and nutrient use efficiency of the plant had been altered by Pb stress. Moreover, the effects of Pb stress on nutrient accumulation, distribution and utilization by poplar were much less in alkaline soil compared to acidic soil.

Key words: acidic purple soil, alkaline purple soil, nutrients utilization, Pb stress, Populus deltoids × P. nigra

KANG Li-Na, WU Fu-Zhong, YANG Wan-Qin, TAN Bo, XIA Lei. Effects of Pb stress on C, N and P accumulation and allocation in Populus deltoids × P. nigra seedlings[J]. Chin J Plant Ecol, 2012, 36(6): 578-586.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2012.00578

https://www.plant-ecology.com/EN/Y2012/V36/I6/578

Figures/Tables 5

References 32

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	pH	全氮 Total nitrogen (g·kg^-1)	全磷 Total phosphorous (g·kg^-1)	全钾 Total potassium (g·kg^-1)	有机碳 Organic carbon (g·kg^-1)	全铅 Total plumbum (mg·kg^-1)
酸性紫色土 Acid purple soil	5.77 ± 0.06	2.21 ± 0.08	0.53 ± 0.04	14.54 ± 0.67	19.65 ± 1.05	47.12 ± 3.67
钙质紫色土 Alkaline purple soil	8.33 ± 0.07	3.11 ± 0.09	1.29 ± 0.04	20.97 ± 1.25	14.37 ± 1.37	30.48 ± 2.85

	pH	全氮 Total nitrogen (g·kg^-1)	全磷 Total phosphorous (g·kg^-1)	全钾 Total potassium (g·kg^-1)	有机碳 Organic carbon (g·kg^-1)	全铅 Total plumbum (mg·kg^-1)
酸性紫色土 Acid purple soil	5.77 ± 0.06	2.21 ± 0.08	0.53 ± 0.04	14.54 ± 0.67	19.65 ± 1.05	47.12 ± 3.67
钙质紫色土 Alkaline purple soil	8.33 ± 0.07	3.11 ± 0.09	1.29 ± 0.04	20.97 ± 1.25	14.37 ± 1.37	30.48 ± 2.85

		处理 Treatment	茎积累量 Stem accumulation (g)	叶积累量 Leaf accumulation (g)	细根积累量 Fine root accumulation (g)	粗根积累量 Coarse root accumulation (g)	总积累量 Total accumulation (g)
酸性紫色土 Acid purple soil	C	CK	24.39 ± 3.00^Aa	12.67 ± 2.12^Aa	4.86 ± 0.46^Aa	23.38 ± 0.96^Aa	65.30 ± 3.65^Aa
		T1	20.51 ± 2.07^Aab	7.96 ± 0.86^Ab	9.18 ± 1.24^Ab	18.72 ± 1.48^Ab	56.37 ± 1.53^Ab
		T2	16.59 ± 1.64^Ab	9.86 ± 0.76^Ab	4.08 ± 1.42^Aa	19.61 ± 0.79^Ab	50.15 ± 0.43^Ac
		T3	12.30 ± 1.62^Ac	5.24 ± 1.64^Ac	3.21 ± 0.17^Aa	14.35 ± 1.55^Ac	35.10 ± 1.82^Ad
	N	CK	0.31 ± 0.07^Aa	0.33 ± 0.02^Aa	0.08 ± 0.01^Aa	0.26 ± 0.05^Aa	0.98 ± 0.10^Aa
		T1	0.25 ± 0.01^Aab	0.22 ± 0.06^Abc	0.14 ± 0.02^Ab	0.18 ± 0.03^Ab	0.79 ± 0.04^Ab
		T2	0.26 ± 0.07^Aab	0.29 ± 0.05^Aab	0.06 ± 0.03^Aa	0.26 ± 0.04^Aa	0.87 ± 0.11^Aab
		T3	0.20 ± 0.02^Ab	0.18 ± 0.08^Ac	0.07 ± 0.01^Aa	0.16 ± 0.04^Ab	0.62 ± 0.06^Ac
	P	CK	0.074 ± 0.012^Aa	0.047 ± 0.007^Aa	0.021 ± 0.002^Aa	0.044 ± 0.007^Aa	0.186 ± 0.011^Aa
		T1	0.055 ± 0.009^Abc	0.032 ± 0.004^Ab	0.039 ± 0.007^Ab	0.041 ± 0.010^Aa	0.166 ± 0.022^Ab
		T2	0.057 ± 0.009^Ab	0.036 ± 0.006^Ab	0.019 ± 0.005^Aa	0.032 ± 0.011^Ab	0.144 ± 0.021^Ac
		T3	0.043 ± 0.002^Ac	0.019 ± 0.005^Ac	0.014 ± 0.001^Aa	0.019 ± 0.003^Ac	0.095 ± 0.004^Ad
钙质紫色土 Alkaline purple soil	C	CK	36.12 ± 2.00^Ba	14.09 ± 1.85^Aa	7.78 ± 0.76^Aa	19.58 ± 2.36^Aa	77.57 ± 1.78^Ba
		T1	34.25 ± 2.70^Ba	12.33 ± 1.37^Aab	4.47 ± 0.76^Bb	17.26 ± 1.16^Aab	68.31 ± 4.47^Bb
		T2	27.21 ± 1.66^Bb	10.49 ± 0.34^Abc	4.35 ± 0.57^Ab	15.79 ± 0.81^Bb	57.84 ± 2.15^Bc
		T3	16.38 ± 2.15^Bc	8.58 ± 0.41^Bc	2.69 ± 0.29^Ac	11.27 ± 0.40^Bc	38.92 ± 2.18^Bd
	N	CK	0.46 ± 0.08^Aa	0.34 ± 0.17^Aa	0.13 ± 0.01^Ba	0.25 ± 0.08^Aa	1.18 ± 0.10^Aa
		T1	0.40 ± 0.06^Ba	0.40 ± 0.06^Ba	0.08 ± 0.02^Bb	0.20 ± 0.04^Aab	1.08 ± 0.09^Bab
		T2	0.37 ± 0.08^Aab	0.32 ± 0.07^Aa	0.07 ± 0.01^Ab	0.19 ± 0.02^Bab	0.95 ± 0.16^Ab
		T3	0.25 ± 0.04^Ab	0.27 ± 0.02^Aa	0.05 ± 0.01^Ab	0.12 ± 0.01^Ab	0.69 ± 0.04^Ac
	P	CK	0.101 ± 0.007^Ba	0.043 ± 0.013^Aa	0.033 ± 0.007^Ba	0.038 ± 0.010^Aa	0.216 ± 0.014^Aa
		T1	0.085 ± 0.007^Bb	0.040 ± 0.005^Aa	0.020 ± 0.006^Bb	0.034 ± 0.006^Aa	0.179 ± 0.014^Ab
		T2	0.071 ± 0.011^Ac	0.033 ± 0.002^Ab	0.018 ± 0.003^Ab	0.027 ± 0.001^Bb	0.149 ± 0.009^Ac
		T3	0.038 ± 0.003^Ad	0.022 ± 0.001^Ac	0.009 ± 0.001^Bc	0.015 ± 0.002^Bc	0.084 ± 0.001^Bd

		处理 Treatment	茎积累量 Stem accumulation (g)	叶积累量 Leaf accumulation (g)	细根积累量 Fine root accumulation (g)	粗根积累量 Coarse root accumulation (g)	总积累量 Total accumulation (g)
酸性紫色土 Acid purple soil	C	CK	24.39 ± 3.00^Aa	12.67 ± 2.12^Aa	4.86 ± 0.46^Aa	23.38 ± 0.96^Aa	65.30 ± 3.65^Aa
		T1	20.51 ± 2.07^Aab	7.96 ± 0.86^Ab	9.18 ± 1.24^Ab	18.72 ± 1.48^Ab	56.37 ± 1.53^Ab
		T2	16.59 ± 1.64^Ab	9.86 ± 0.76^Ab	4.08 ± 1.42^Aa	19.61 ± 0.79^Ab	50.15 ± 0.43^Ac
		T3	12.30 ± 1.62^Ac	5.24 ± 1.64^Ac	3.21 ± 0.17^Aa	14.35 ± 1.55^Ac	35.10 ± 1.82^Ad
	N	CK	0.31 ± 0.07^Aa	0.33 ± 0.02^Aa	0.08 ± 0.01^Aa	0.26 ± 0.05^Aa	0.98 ± 0.10^Aa
		T1	0.25 ± 0.01^Aab	0.22 ± 0.06^Abc	0.14 ± 0.02^Ab	0.18 ± 0.03^Ab	0.79 ± 0.04^Ab
		T2	0.26 ± 0.07^Aab	0.29 ± 0.05^Aab	0.06 ± 0.03^Aa	0.26 ± 0.04^Aa	0.87 ± 0.11^Aab
		T3	0.20 ± 0.02^Ab	0.18 ± 0.08^Ac	0.07 ± 0.01^Aa	0.16 ± 0.04^Ab	0.62 ± 0.06^Ac
	P	CK	0.074 ± 0.012^Aa	0.047 ± 0.007^Aa	0.021 ± 0.002^Aa	0.044 ± 0.007^Aa	0.186 ± 0.011^Aa
		T1	0.055 ± 0.009^Abc	0.032 ± 0.004^Ab	0.039 ± 0.007^Ab	0.041 ± 0.010^Aa	0.166 ± 0.022^Ab
		T2	0.057 ± 0.009^Ab	0.036 ± 0.006^Ab	0.019 ± 0.005^Aa	0.032 ± 0.011^Ab	0.144 ± 0.021^Ac
		T3	0.043 ± 0.002^Ac	0.019 ± 0.005^Ac	0.014 ± 0.001^Aa	0.019 ± 0.003^Ac	0.095 ± 0.004^Ad
钙质紫色土 Alkaline purple soil	C	CK	36.12 ± 2.00^Ba	14.09 ± 1.85^Aa	7.78 ± 0.76^Aa	19.58 ± 2.36^Aa	77.57 ± 1.78^Ba
		T1	34.25 ± 2.70^Ba	12.33 ± 1.37^Aab	4.47 ± 0.76^Bb	17.26 ± 1.16^Aab	68.31 ± 4.47^Bb
		T2	27.21 ± 1.66^Bb	10.49 ± 0.34^Abc	4.35 ± 0.57^Ab	15.79 ± 0.81^Bb	57.84 ± 2.15^Bc
		T3	16.38 ± 2.15^Bc	8.58 ± 0.41^Bc	2.69 ± 0.29^Ac	11.27 ± 0.40^Bc	38.92 ± 2.18^Bd
	N	CK	0.46 ± 0.08^Aa	0.34 ± 0.17^Aa	0.13 ± 0.01^Ba	0.25 ± 0.08^Aa	1.18 ± 0.10^Aa
		T1	0.40 ± 0.06^Ba	0.40 ± 0.06^Ba	0.08 ± 0.02^Bb	0.20 ± 0.04^Aab	1.08 ± 0.09^Bab
		T2	0.37 ± 0.08^Aab	0.32 ± 0.07^Aa	0.07 ± 0.01^Ab	0.19 ± 0.02^Bab	0.95 ± 0.16^Ab
		T3	0.25 ± 0.04^Ab	0.27 ± 0.02^Aa	0.05 ± 0.01^Ab	0.12 ± 0.01^Ab	0.69 ± 0.04^Ac
	P	CK	0.101 ± 0.007^Ba	0.043 ± 0.013^Aa	0.033 ± 0.007^Ba	0.038 ± 0.010^Aa	0.216 ± 0.014^Aa
		T1	0.085 ± 0.007^Bb	0.040 ± 0.005^Aa	0.020 ± 0.006^Bb	0.034 ± 0.006^Aa	0.179 ± 0.014^Ab
		T2	0.071 ± 0.011^Ac	0.033 ± 0.002^Ab	0.018 ± 0.003^Ab	0.027 ± 0.001^Bb	0.149 ± 0.009^Ac
		T3	0.038 ± 0.003^Ad	0.022 ± 0.001^Ac	0.009 ± 0.001^Bc	0.015 ± 0.002^Bc	0.084 ± 0.001^Bd

	处理 Treatment	叶C/N Leaf C/N	叶N/P Leaf N/P	总C/N Total C/N	总N/P Total N/P	N利用效率NUE_N (g mass·g^-1)	P利用效率NUE_P (g mass·g^-1)
酸性紫色土 Acid purple soil	CK	38.08 ± 4.16^Aa	7.13 ± 0.68^Aa	66.76 ± 6.06^Aa	5.31 ± 0.53^Aab	133.39 ± 12.46^Aab	703.27 ± 5.30^Aa
	T1	35.86 ± 4.86^Aa	7.13 ± 1.18^Aa	71.27 ± 3.65^Aa	4.76 ± 0.29^Aa	148.85 ± 7.37^Aa	707.47 ± 13.38^Aa
	T2	34.81 ± 5.24^Aa	7.87 ± 1.11^Aa	57.78 ± 6.69^Ab	6.07 ± 0.69^Abc	122.78 ± 13.62^Ab	738.53 ± 8.11^Ab
	T3	30.94 ± 8.43^Aa	9.47 ± 2.13^Aa	57.15 ± 3.43^Ab	6.50 ± 0.31^Ac	124.04 ± 8.52^Ab	805.04 ± 24.31^Ab
钙质紫色土 Alkaline purple soil	CK	34.91 ± 2.06^Aa	7.60 ± 3.35^Ba	65.96 ± 4.57^Aa	5.47 ± 0.28^Aa	135.75 ± 8.22^Aa	741.29 ± 11.33^Ba
	T1	30.88 ± 1.85^Aa	10.11 ± 0.7^Bab	63.60 ± 2.57^Ba	6.01 ± 0.06^Ba	131.61 ± 2.00^Bab	791.22 ± 9.19^Bb
	T2	33.47 ± 8.21^Aa	9.94 ± 2.18^Aa	61.98 ± 11.86^Aa	6.40 ± 1.03^Aa	119.85 ± 4.28^Abc	821.10 ± 4.73^Bc
	T3	31.59 ± 1.65^Aa	12.31 ± 0.35^Bb	56.57 ± 0.63^Aa	8.20 ± 0.27^Bb	122.79 ± 3.83^Ac	1005.94 ± 1.56^Bd