EFFECTS OF NACL STRESS ON ACTIVE OXYGEN METABOLISM AND MEMBRANE STABILITY IN PISTACIA VERA SEEDLINGS

doi:10.17521/cjpe.2005.0131

Abstract

Abstract:

Pistachio (Pistacia vera) is one of the most important dried fruit trees in the world and is mainly cultivated in Xinjiang, China. However, soil salinization is one of the main limiting factors for its promising potential of development. In this experiment, the effects of NaCl stress on physiological and biochemical characteristics were investigated in seedlings of two cultivars of Pistacia vera, `Changguo' and `Kerman'. Seedlings were grown in pots and treated with four NaCl concentrations: 50, 150, 250 and 500 mmol·L^-1. The membrane permeability, malondialdehyde content (MDA), superoxide dismutase (SOD) activity, catalase (CAT) activity and peroxidase (POD) activity in the leaves of these two cultivars were measured and compared after 5, 10 and 20 days of NaCl treatments.
The experimental results showed that the membrane permeability and MDA content in both cultivars increased considerably with the increasing NaCl stress, which aggravated the degree of membrane lipid peroxidation and injured the membrane stability. The membrane permeability and MDA content in the `Changguo' cultivar increased more quickly than the `Kerman' cultivar and thus experienced greater damage at higher NaCl concentrations. The activities of SOD, CAT and POD had similar trends for both cultivars, first increasing with NaCl concentrations up to 250 mmol·L^-1 and then decreasing at the highest NaCl stress of 500 mmol·L^-1. The coordinated changes of activities among the antioxidant enzymes of SOD, POD and CAT could scavenge active oxygen and maintain a balance of active oxygen accumulation in cells to protect membrane structure. The results also showed that the damage by NaCl stress to the membrane structure and function for both cultivars were mitigated considerably 20 days after the treatments were initiated. There were significant correlations among the membrane permeability, MDA content and the activity of SOD in both cultivars, which implied that the membrane permeability in the plant cells had a close relation with MDA content and oxygen free radical content, as well as the activity of antioxidant enzymes. Together, these results indicated that the `Kerman' cultivar has higher antioxidant levels and greater salt-tolerance than the `Changguo' cultivar of Pistachio.

Key words: NaCl stress, Pistacia vera, Membrane permeability, MDA, SOD, CAT, POD

YUAN Lin, KARIM Ali, ZHANG Li-Quan. EFFECTS OF NACL STRESS ON ACTIVE OXYGEN METABOLISM AND MEMBRANE STABILITY IN PISTACIA VERA SEEDLINGS[J]. Chin J Plant Ecol, 2005, 29(6): 985-991.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2005.0131

https://www.plant-ecology.com/EN/Y2005/V29/I6/985

Figures/Tables 4

References 23

[1]	Akita S, Cabuslay GS (1990). Physiological basis of differential response to salinity in rice cultivars. Plant and Soil, 123,277-294.
[2]	Ashworth LJ Jr, Gaona SA, Surber E (1985). Nutritional diseases of pistachio trees: potassium and phosphorus deficiencies and chloride and boron toxicities. Phytopathology, 75,1084-1091.
[3]	Battacharjee S, Mukherjee AK (1996). Ethylene evolution and membrane lipid peroxidation as indicators of salt injury in leaf tissues of amaranths seedlings. Indian Journal of Experimental Biology, 34,279-281.
[4]	Bowler C, van Montagu M, Inze D (1992). Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecule Biology, 43,83-116.
[5]	Chen LS (陈立松), Liu XH (刘星辉) (2003). Fruit Tree Anti-Stress Physiology (果树逆境生理). China Agriculture Press, Beijing,16-256. (in Chinese)
[6]	Chen Q (陈沁), Liu YL (刘友良) (2000). Effect of glutathione on active oxygen scavenging system in leaves of barley seedlings under salt stress. Acta Agronomica Sinica (作物学报), 26,365-371. (in Chinese with English abstract)
[7]	Dionisio-Sese ML, Tobita S (1998). Antioxidant responses of rice seedlings to salinity stress. Plant Science, 135,1-9. DOI URL
[8]	Editorial Committee of Flora of China, Chinese Academy of Sciences (中国科学院中国植物志编辑委员会) (1980). Flora of China (Vol.45,No.1)(中国植物志45卷第一期). Science Press, Beijing,94-96. (in Chinese)
[9]	Gueta-Dahan Y, Yaniv Z, Zilinskas BA, Ben-Hayyim G (1997). Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus. Planta, 203,460-469. DOI URL PMID
[10]	Hernandez JA, Jimenez A, Mullineaux P, Sevilla F (2000). Tolerance of pea ( Pisum sativum L.) to long term salt stress is associated with induction of antioxidant defenses. Plant Cell Biology, 23,853-862.
[11]	Li HS (李合生) (2000). The Principle and Experimental Technology of Plant Physiology and Biochemistry (植物生理生化实验原理和技术). Higher Education Press, Beijing,164-169. (in Chinese)
[12]	Lin QF (林栖风), Li GY (李冠一) (2000). The status and advances of plant salt-tolerance. Bioengineering Evolvement (生物工程进展), 20(2),20-25. (in Chinese with English abstract)
[13]	Pan RC (潘瑞炽), Dong YD (董愚得) (1995). Plant Physiology (植物生理学)3rd edn. Higher Education Press, Beijing,318-335. (in Chinese)
[14]	Picchioni GA, Miyamota S (1990). Salt effects on growth and ion uptake of pistachio rootstock seedlings. Journal of the American Society for Horticultural Science, 115,647-653.
[15]	Sepskhah AR, Maftoin M (1988). Relative salt tolerance of pistachio cultivars. Horticulture Science, 63,157-162.
[16]	Wang BS (王宝山), Yao DY (姚敦义) (1993). The effect of salt tolerance on membrane permeability、lipid peroxidization and SOD activity of callus in Elacagnus angustifolia. Journal of Hebei Agricultural University (河北农业大学学报), 16(3),20-24. (in Chinese)
[17]	Wang HC (王洪春) (1981). Plant adaptation to stresses. Plant Physiology Communications (植物生理学通讯), 6,72-73. (in Chinese)
[18]	Wang JH (王继和), Yang ZH (杨自辉) (2001). Research of the comprehensive technologies on transformation and utilization of saline land in arid areas. Chinese Journal of Eco-Agriculture (中国生态农业学报), 9,64-66. (in Chinese with English abstract)
[19]	Wang Z (王忠) (2000). Plant Physiology (植物生理学). China Agriculture Press, Beijing,422-439. (in Chinese)
[20]	Yu Z, Rengel Q (1999). Drought and salinity differentially influence activities of superoxide dismutase in narrow leafed lupines. Plant Science, 141,1-11.
[21]	Zhang KC (张开春) (2000). The Handbook of Fruit Tree Breeding (果树育苗手册). China Agriculture Press, Beijing,302-304. (in Chinese)
[22]	Zhao SJ (赵氏杰), Xu CC (许长成), Zou Q (邹琦), Meng QW (孟庆伟) (1994). Improvements of method for measurement of malondialdehyde in plant tissues. Plant Physiology Communications (植物生理学通讯), 30,207-210. (in Chinese)
[23]	Zhu GL (朱广廉), Zhong HW (钟海文), Zhang AQ (张爱琴) (1990). Experiments of Plant Physiology (植物生理学实验). Peking University Press, Beijing,252-254. (in Chinese)

	膜透性 Membrane permeability	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
膜透性Membrane permeability	1
MDA含量MDA content	0.876^**	1
SOD活性SOD activity	0.557^*	0.667^**	1
CAT活性CAT activity	0.107	0.378	0.140	1
POD活性POD activity	0.368	0.572^*	0.742^**	0.521^*	1

	膜透性 Membrane permeability	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
膜透性Membrane permeability	1
MDA含量MDA content	0.876^**	1
SOD活性SOD activity	0.557^*	0.667^**	1
CAT活性CAT activity	0.107	0.378	0.140	1
POD活性POD activity	0.368	0.572^*	0.742^**	0.521^*	1

	膜透性 Membrane permeability	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
膜透性Membrane permeability	1
MDA含量MDA content	0.871^**	1
SOD活性SOD activity	0.741^**	0.640^**	1
CAT活性CAT activity	0.336	0.314	0.682^**	1
POD活性POD activity	0.518^*	0.436	0.909^**	0.693^**	1

	膜透性 Membrane permeability	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity
膜透性Membrane permeability	1
MDA含量MDA content	0.871^**	1
SOD活性SOD activity	0.741^**	0.640^**	1
CAT活性CAT activity	0.336	0.314	0.682^**	1
POD活性POD activity	0.518^*	0.436	0.909^**	0.693^**	1