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Effects of 3,4-dihydroxy acetophenone stress on changes in the content of endogenous plant hormones during seed germination in Picea schrenkiana ssp. tianschanica
Received date: 2013-08-05
Accepted date: 2013-10-08
Online published: 2013-12-04
Aims Picea schrenkiana ssp. tianschanica is a major tree species in forest ecosystems in the Xinjiang Uygur Autonomous Region, West China, and plays an important role in the regional water conservation. 3,4-dihydroxy acetophenone (DHAP) is a principal autotoxic substance occurring in foliage and litter of P. schrenkiana ssp. tianschanica that causes suppressed natural regeneration in P. schrenkiana ssp. tianschanica forests. The objectives of this study were to investigate changes in the content of endogenous plant hormones during seed germination in P. schrenkiana ssp. tianschanica as affected by DHAP, and to elucidate the underlying physiological mechanisms in the autotoxic effects.
Methods We investigated the inter-relationships among DHAP treatment, seed germination, and changes in the concentration of endogenous plant hormones by measuring the contents of zeatin (ZT), gibberellin (GA3), indole-acetic acid (IAA), and abscisic acid (ABA) during germination process in P. schrenkiana ssp. tianschanica seeds with or without DHAP treatment. The concentrations of plant hormones in germinating seeds were measured with the method of ultra performance liquid chromatography (UPLC).
Important findings Results showed that the impact of DHAP on seed germination in P. schrenkiana ssp. tianschanica depended on treatment concentrations, in the order of 5.0 mmol·L-1 > 0.1 mmol·L-1 > 1.0 mmol·L-1 > control; treatments with DHAP reduced the concentrations of ZT and GA3, delayed the peak occurrence of GA3, and increased the concentration of ABA with a delayed peak occurrence under treatments of 1.0 and 5.0 mmol·L-1 DHAP. Treatment with 5.0 mmol·L-1 DHAP for 3-6 days greatly increased the level of IAA. 1-6 days of treatments with DHAP reduced the ZT/(GA3 + IAA) ratio, and increased the IAA/ZT ratio and ABA/ZT ratio; the ABA/(ZT + GA3 + IAA) ratio increased at 0.1 mmol·L-1 DHAP and decreased at 5.0 mmol·L-1 DHAP. It is suggested that DHAP treatment causes changes in the content and balance of endogenous plant hormones, leading to inhibition or delaying in seed germination in P. schrenkiana ssp. tianschanica.
CHEN Zhi-Ying, RUAN Xiao, ZHANG Yu-Zhu, PAN Cun-De, WANG Qiang . Effects of 3,4-dihydroxy acetophenone stress on changes in the content of endogenous plant hormones during seed germination in Picea schrenkiana ssp. tianschanica[J]. Chinese Journal of Plant Ecology, 2013 , 37(12) : 1114 -1122 . DOI: 10.3724/SP.J.1258.2013.00114
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