Chin J Plan Ecolo ›› 2012, Vol. 36 ›› Issue (12): 1286-1292.doi: 10.3724/SP.J.1258.2012.01286

• Research Articles • Previous Articles    

Effects of fractionated nitrogen application on leaf senescence and 15 N-urea uptake and utilization of apple rootstock―Malus hupenhensis

DING Ning, JIANG Yuan-Mao*, WEI Shao-Chong, CHEN Qian, and GE Shun-Feng   

  1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China
  • Received:2012-04-10 Revised:2012-09-24 Online:2012-11-28 Published:2012-12-01
  • Contact: JIANG Yuan-Mao


Aims The effects of fractionated nitrogen (N) fertilization (once, twice and thrice) on leaf senescence and nitrogen uptake and utilization of potted Malus hupenhensis were explored using the 15N labeling technique.
Methods We measured growth parameters such as plant height, stem diameter, leaf area and chlorophyll content (SPAD), enzyme parameters such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in leaves and 15N parameters such as uptake and utilization for potted M. hupehensis under different fractionated N applications.
Important findings Plant height, stem diameter, leaf area and SPAD were highest at early growth stage for once fractionated application and lower for twice fractionated application, and lowest for thrice fractionated application. At middle growth stage, twice fractionated application showed the highest values, while once fractionated application exhibited the lowest values. At the late growth stage, thrice fractionated application was the highest, while once fractionated application was the lowest. The leaf SOD, POD and CAT activities were highest for once fractionated application at early growth stage and lowest for thrice fractionated application. At the middle growth stage, twice fractionated application was the highest, whereas once fractionated application was lowest. At the late growth stage, thrice fractionated application showed the highest values, while once fractionated application had the lowest values. The 15N derived from fertilizer (Ndff) value in different organs was significantly different. Thrice fractionated application demonstrated higher values than either of once and twice fractionated application at the late growth stage. Plant total N, 15N uptake and N-usage efficiency were highest for thrice fractionated application at late growth stage. This indicates that thrice fractionated application not only can enhance the total N content of leaves and delay leaf senescence, but also can increase the N-usage efficiency.

[1] Bao SD (鲍士旦) (2000). Soil Agricultural-Chemical Analysis (土壤农化分析) 3rd Ed. China Agriculture Press, Beijing.  CrossRef
[2] De Jong TM, Day KR, Johnson RS (1989). Partitioning of leaf nitrogen with respect to with in canopy light exposure and nitrogen availability in peach [J]. Trees, 3(2): 89-95.CrossRef
[3] Fang XJ (房祥吉), Jiang YM (姜远茂), Peng FT (彭福田) (2011). Effects of Different Sand/Soil Ratio on Growth and -(15)N Absorption, Utilization and Loss of Potted Malus hupenhensis [J]. Journal of Soil and Water Conservation (水土保持学报), 25(4): 131-134.  CrossRef
[4] Gao XL (高小丽), Sun JM (孙健敏), GaoJF (高金峰), Feng BL ( 冯佰利), Chai Y (柴岩), Jia ZK (贾志宽) (2008). Leaf aging and reactive oxygen metabolism in different genotypes of mung bean[J]. Scientia Agricultura Sinica (中国农业科学), 41(9): 2873-2880.  CrossRef
[5] GOU L (勾玲), Yan J (闫洁), Han CL (韩春丽) (2004). Effects of nitrogen rates on photosynthetic characteristics and yield of high-yielding cotton in Xinjiang[J]. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 10(5): 488-493.  CrossRef
[6] Gu MR (顾曼如) (1990). The application of 15N in the study of nitrogen nutrition on apples [J]. China Fruits (中国果树), (2): 46-48.  CrossRef
[7] Gu MR (顾曼如), Zhang RZ (张若杼), Shu HR (束怀瑞), Li WW (黎文文), Huang HC (黄花成) (1981). A study on the nitrogen nutrition of apple trees-The variation of nitrogen nutrition within the plant in a year’s cycle. Acta Horticulture Sinica (园艺学报), 8(4): 21-28.  CrossRef
[8] Guo G L (郭改玲), Liu KL (刘克礼), Gao JL (高聚林) (2006). Effect of nitrogen application on flag leaf senescence and yield of spring wheat[J]. Journal of Triticeace Crops (麦类作物学报), 26(5): 126-129.  CrossRef
[9] Han ZH (韩振海), Zeng X (曾骧), Wang FJ (王福钧) (1992). Effects of autumm foliar application of 15N-urea and several PGR on N storage and reuse in apple[J]. Acta Horticulture Sinica (园艺学报), 19(1): 15-21. CrossRef
[10] Jiang YM (姜远茂), Peng FT (彭福田), Zhang HY (张宏彦), Li XL (李晓林), Zhang FS (张福锁) (2001). Status of organic matter and nutrients in Shandong orchard soil [J]. Chinese Journal of Soil Science, 32(4): 167-169. CrossRef
[11] Khemira H (1991). Influence of canopy orientation on fruiting of ‘Anjou’ pears and post harvest urea spray on ovule longevity and fruit set of‘Comice’pears. Master Dissertation. Corvallis, OR: Oregon State University, 1991.  CrossRef
[12] Hobbie L, Estelle M (1994). Genetic approaches to auxin action. Plant Cell Environ, 17(5), 525-540.  CrossRef
[13] Li WQ (李文庆), Zhang M (张民), Shu HR (束怀瑞) (2002). The physiological effects of nitrogen on fruit trees. Journal of Shandong Agricultural University (Natural Science) (山东农业大学学报)(自然科学版), 33(1), 96-100. CrossRef
[14] Lin ZF (林植芳), Li SS (李双顺), Lin GZ (林桂珠), Sun GC (孙谷畴), Guo JY (郭俊彦) (1984). Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves. Acta Botanica Sinica (植物学报), 26(6): 605-615. CrossRef
[15] Oaks A, Aslam M, Boesel Z (1997). Ammonium and amino acid as regulators of nitrate reeducate in corn roots. Plant Physiol, 59: 391-394.  CrossRef
[16] Peng FT (彭福田), Zhang Q (张青), Jiang YM (姜远茂), Peng Y (彭勇), Zhou P (周鹏) (2006). Effect of nitrogen application on nitrogen absorption, distribution and yield of strawberry[J]. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 12(3): 400-405. CrossRef
[17] Peng FT(彭福田), Jiang YM(姜远茂), Gu MR(顾曼如), Shu HR(束怀瑞) (2003). Effect of nitrogen on apple fruit hormone changing trends and development[J]. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 9(2): 208-213. CrossRef
[18] Raese JT, Drake SR, Curry EA (2007). Nitrogen fertilizer influences fruit quality, soil nutrients and cover crops, leaf color and nitrogen content, biennial bearing and cold hardiness of “Golden Delicious”. Journal of Plant Nutrition, 30: 1585-1604.  CrossRef
[19] Shi CY (史沉鱼), Li XM (李向民), Li XD (李晓东), Zhang R (张蓉) (2010). Two fertilizer treatments on the aroma components in Fuji apples. Northern Horticulture (北方园艺), (3), 1-3. CrossRef
[20] Stassen PJC, Terblanche JH, Strydom DK (1981). The effect of time and rate of nitrogen application on development and composition of peach trees[J]. Agoplantae, 13: 55-61.  CrossRef
[21] Wang AG (王爱国), Luo GH (罗广华), Shao CB (邵从本), Wu SJ (吴淑君), Guo JY (郭俊彦) (1983). A study on the superoxide dismutase of soybean seeds. Acta Photophysiologica Sinica (植物生理学报), 9(1): 77-84. CrossRef
[22] Wang XY (王晓云), Li XD (李向东), Zhou Q (邹琦) (2001). Regulation effects of nitrogen application on the polyamine metabolism and senescence of peanut leaves. Acta Agronomica Sinica (作物学报), 27(4): 442-446. CrossRef
[23] Wei HY (魏海燕), Zhang HC (张洪程), Ma Q (马群) (2010). Characteristics of leaf senescence in rice genotypes with different nitrogen use efficiencies[J]. Acta Agronomica Sinica (作物学报), 36(4): 645-654. CrossRef
[24] Xu YX (徐艳霞), Wei ZB (魏占彬), Wang TL (王天亮) (2009). Effect of different fertilization methods on growth and grain yield of summer maize. Anhui Agricultural Science Bulletin (安徽农学通报), 15(18), 54-55. CrossRef
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[2] Ningguang Dong, Ying Gao, Wei Wang, Weilun Yin, Dong Pei. Immunogold Silver Localization of Indole-3-acetic Acid (IAA) During the Rhizogenesis of In Vitro Poplar[J]. Chin Bull Bot, 2011, 46(3): 324 -330 .
[3] HONG Wei CAO Jia-Shu. The Function of FLC in Vernalization Process[J]. Chin Bull Bot, 2002, 19(04): 406 -411 .
[4] . Development and Utilization of Plant Resources II[J]. Chin Bull Bot, 1994, 11(02): 53 -57 .
[5] FAN Qing-Shu ZHAO Jian-Cheng YU Shu-Hong LI Xiu-Qin. Progress in Study on Spore Germination and Protonema Development of the Bryophytes[J]. Chin Bull Bot, 2003, 20(03): 280 -286 .
[6] LIU Jian-Wu LIU Ning. The Progress in Study on Development of Fern Gametophytes and Differentiation of Sex Organ[J]. Chin Bull Bot, 2001, 18(02): 149 -157 .
[7] An Cheng-xi. Studies on the Chemical Constituents of Essential of Aiania-Tanuifolia[J]. Chin Bull Bot, 1997, 14(增刊): 74 -76 .
[8] Nie Wei. Observation on some Biological Characteristics of Juncellus serotinus in Transplanted Rice[J]. Chin Bull Bot, 1988, 5(01): 34 -36 .
[9] . Mechanism of Plant Photosynthetic Acclimation to Elevated Atmospheric CO2[J]. Chin Bull Bot, 2005, 22(04): 486 -493 .