Chin J Plan Ecolo ›› 2013, Vol. 37 ›› Issue (1): 70-79.doi: 10.3724/SP.J.1258.2013.00008

• Research Articles • Previous Articles     Next Articles

Inoculation two azotobacter enhancing osmotic stress resistance and growth in wheat seedling

LIU Hua-Wei1,2*, LIN Xiao-Jun1,2, SUN Chao1,2, LI Qiang1,2, YANG Hu1,2, GUO Ai-Guang1,2**   

  1. 1College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China;

    2State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi 712100, China
  • Received:2012-09-10 Revised:2012-11-08 Online:2013-01-15 Published:2013-01-01
  • Contact: GUO Ai-Guang


Aims The seedling stage is the key stage of matter and energy accumulation in the wheat life cycle. Therefore, drought during the seedling stage affects population formation in late stages. In this study, wheat seedlings were inoculated with azotobacters Azorhizobium caulinodans ‘ORS571’ and Azospirillum brasilense ‘Yu62’.
Methods Wheat seedlings germination was screened in normal conditions and with PEG 6000 osmotic stress using seedlings inoculated with azotobacters. Root volume, relative water content (RWC), proline content and soluble protein content of seedling laminas were determined under PEG drought stress using seedlings inoculated with azotobacters on laminas.
Important findings The germination rate of wheat seedlings was significantly increased under drought stress when inoculated with azotobacters. Moreover, wheat seedlings inoculated with mixed azotobacters have more obvious growth promotion than when inoculated with a single azotobacter. The former laminas proline content, relative water content, proline content and soluble protein content had increased. The results showed that drought resistance of wheat seedlings was improved when inoculated with mixed azotobacters, which provided the foundation for further study of azotobacter-wheat interaction under drought stress.

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