Chin J Plan Ecolo ›› 2009, Vol. 33 ›› Issue (2): 302-310.DOI: 10.3773/j.issn.1005-264x.2009.02.007

• Research Articles • Previous Articles     Next Articles

PATERNITY ANALYSIS OF OPEN- AND CONTROL-POLLINATED SEEDS COLLECTED FROM A SEED ORCHARD OF PINUS TABULAEFORMIS

ZHANG Dong-Mei1; SUN Pei-Guang2; SHEN Xi-Huan3; RU Guang-Xin2   

  1. 1Shanghai Landscape Gardening Research Institute, Shanghai 200232, China;2 College of Forestry and Horticulture, Henan Agricultural University, Zhengzhou 450002, China;3College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
  • Online:2009-03-31 Published:2009-03-31
  • Contact: ZHANG Dong-Mei

Abstract: Aims Our objective was to develop theory for design and reestablishment of high-generation seed orchard by discussing the relationships between pollen dispersal distances and genetic composition of seed orchard progeny under open- and control-pollinated situations.
Methods Open- and control-pollinated seeds were collected from two maternal trees. Microsatellites markers (SSRs) were used in paternity analysis. A total of 281 progenies were analyzed, of which the paternity could be accurately identified for just 126. Paternity inference was conducted using CERVUS software.
Important findings Analysis of open-pollinated seeds of two clones, Nos.11 and 24, showed the effective dispersal distance of pollen is within 30 m and the longest dispersal distance is 85 m. 11.1%–12.8% of pollen-father comes within a radius of 10 m from the seed tree, 37.0%–40.4% within 10–20 m and 17.2%–22.2% within a radius of 20-30 m. In order to discover the key factor to selection fertilization, the paternity analysis of control-pollinated seeds for two clones, Nos.11 and 24, was estimated too. Four clones. Nos. 50, 7, 25 and 33, applied 50.62% and 61.54% pollen to control-pollinated seeds collected from two mother trees, but the reason is not yet clear. We analyzed the influence of pollen size, weight and vigor on selection fertilization, but no significant correlation was found. We suggested detailed study of influence of pollen-tube growth rate on selection fertilization.