硝酸钙胁迫对营养液栽培嫁接茄子叶片抗坏血酸-谷胱甘肽循环的影响

doi:10.3773/j.issn.1005-264x.2008.05.007

摘要/Abstract

摘要：

以日本引进的设施专用耐盐茄(Solanum melongena)品种‘Torvum Vigor’为砧木, 栽培茄(S. torvum)品种‘苏崎茄’为接穗, 用营养液栽培, 对80 mmol·L^-1Ca(NO₃)₂胁迫下茄子嫁接苗和自根苗叶片抗坏血酸-谷胱甘肽循环系统中抗氧化酶活性和抗氧化物及H₂O₂含量进行比较。结果表明, Ca(NO₃)₂胁迫下茄子幼苗叶片H₂O₂含量有所增加, 但嫁接苗叶片H₂O₂含量显著低于自根苗。Ca(NO₃)₂胁迫下嫁接苗叶片抗氧化酶(APX、DHAR和GR)活性、AsA和GSH再生率、氧化还原力(AsA/DHA值和GSH/GSSG值)均显著高于自根苗。综上所述, Ca(NO₃)₂胁迫下嫁接苗保持良好的AsA-GSH循环效率, 清除H₂O₂效率较高, 细胞受氧化损伤程度较轻, 表现出较强的耐盐性。

关键词: 嫁接茄子, Ca(NO₃)₂胁迫, 抗坏血酸-谷胱甘肽循环, 营养液栽培

Abstract:

Aims Oxidative stress is one of the responses of plants to salt stress, which causes the production of hydrogen peroxide (H₂O₂). Plants grafted on salt resistant rootstocks could improve their tolerance to salt stress because of the vigorous root system of rootstocks. However, little is known about the relationship between salt tolerance of grafted plants and the scavenging system of H₂O₂ under Ca(NO₃)₂ stress. Our objective was to investigate the mechanism and roles of ascorbate-glutathione metabolism system in salt tolerance of grafted eggplant under Ca(NO₃)₂ stress, including ascorbate pool, glutathione pool and the activities of antioxidant enzymes.

Methods We grafted cultivar ‘Suqi qie’ ( Solanum melongena) onto rootstock of ‘Torvum Vigor’ ( S. torvum), which is a salt tolerant cultivar introduced from Japan. We compared leaves of hydroponically-grown grafted and own-root eggplant seedlings grown under 80 mmol·L^-1Ca(NO₃)₂ stress for changes of antioxidant enzyme activities, contents of antioxidants in ascorbate-glutathione cycle metabolism and content of H₂O₂.

Important findings Under Ca(NO₃)₂ stress, H₂O₂ content increased in leaves of eggplant seedlings and was significantly lower in leaves of grafted seedlings than own-root seedlings. Also, activities of antioxidant enzymes (APX, DHAR and GR), regenerating rates of AsA and GSH and redox statuses (ratios of AsA/DHA and GSH/GSSG) were significantly higher in leaves of grafted seedlings than own-root seedlings. We concluded that grafted eggplant seedlings had efficient metabolism of ascorbate-glutathione cycle, which scavenged the H₂O₂ rapidly to alleviate the oxidative damage of Ca(NO₃)₂ stress; therefore, grafted seedlings had a stronger tolerance to salt stress.

Key words: grafted eggplant, Ca(NO₃)₂ stress, ascorbate-glutathione cycle, hydroponically-grown

魏国平, 朱月林, 刘正鲁, 张古文, 杨立飞. 硝酸钙胁迫对营养液栽培嫁接茄子叶片抗坏血酸-谷胱甘肽循环的影响. 植物生态学报, 2008, 32(5): 1023-1030. DOI: 10.3773/j.issn.1005-264x.2008.05.007

WEI Guo-Ping, ZHU Yue-Lin, LIU Zheng-Lu, ZHANG Gu-Wen, YANG Li-Fei. EFFECTS OF CALCIUM NITRATE STRESS ON ASCORBATE-GLUTATHIONE CYCLE METABOLISM IN LEAVES OF HYDROPONICALLY-GROWN GRA- FTED EGGPLANT SEEDLINGS. Chinese Journal of Plant Ecology, 2008, 32(5): 1023-1030. DOI: 10.3773/j.issn.1005-264x.2008.05.007

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.05.007

https://www.plant-ecology.com/CN/Y2008/V32/I5/1023

图/表 4

图1 Ca(NO3)2胁迫对茄子嫁接苗和自根苗叶片APX、DHAR和GR活性的影响图中数据为3次重复的平均值±标准误差 The data are means of three replicates ± SE

Fig.1 Effects of Ca(NO3)2 stress on APX, DHAR and GR activities in leaves of grafted and own-root eggplant seedlings

图2 Ca(NO3)2胁迫对茄子嫁接苗和自根苗叶片AsA与DHA含量及AsA/DHA比值的影响图注同图1 Notes see Fig. 1

Fig. 2 Effects of Ca(NO3)2 stress on AsA and DHA contents and AsA/DHA ratio in leaves of grafted and own-root eggplant seedlings

图3 Ca(NO3)2胁迫对茄子嫁接苗和自根苗叶片GSH与GSSG含量及GSH/GSSG比值的影响图注同图1 Notes see Fig. 1

Fig. 3 Effects of Ca(NO3)2 stress on GSH and GSSG contents and GSH/GSSG ratio in leaves of grafted and own-root eggplant seedlings

表1 Ca(NO3)2胁迫对茄子嫁接苗和自根苗叶片H2O2含量(μmol·g-1FW)的影响

Table 1 Effect of Ca(NO3)2 stress on H2O2 content (μmol·g-1FW) in leaves of grafted and own-root eggplant seedlings

处理 Treatment	胁迫时间 Stress time (d)
处理 Treatment	0	3	6	9	12	15
O1	5.08±0.22^a	5.22±0.20^a	5.07±0.30^b	5.08±0.30^c	5.14±0.24^c	5.24±0.27^c
O2	5.04±0.37^a	5.48±0.70^a	6.48±0.17^a	8.51±0.35^a	11.71±1.46^a	13.92±1.19^a
G1	4.82±0.24^a	4.83±0.21^a	5.03±0.14^b	5.17±0.10^c	5.20±0.19^c	5.10±0.24^c
G2	5.05±0.19^a	4.90±0.19^a	5.38±0.09^b	5.97±0.21^b	6.93±0.23^b	8.22±0.34^b

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