Abstract: Gordonia acuminata is a widely distributed tree species. Its secondary metabolism features differ significantly with its geographical distribution. By the method of polyacrylamides membrane chromatography and quantitative analysis, the flavonoids in G. acuminata were analyzed using the samples collected from Mt. Laojun (situated in Wenshan, Yunnan), Danling (in Sichuan ), Xingwen (in Sichuan), Mt. Jinyun (in Sichuan). Sanchahe (in Yangshuo, Guangxi) Sanpan (in Yangshuo,Guangxi), Mt. Gupo (in Hexian,Guangxi). The results show that G. acuminata plants collected from different geographical regions were very different in flavonoids components. Based on the variations in flavonoids components, three kinds of geographical distribution patterns of G. acuminata were distinguished: (1) higher elevation and lower latitude distribution, (2) middle elevation and lower latitude distribution, and (3) middle elevation and middle latitude distribution.