EFFECTS OF PLC/PLD INHIBITOR ON EXPRESSION AND REGULATION OF A NOVEL 69.5 kD HEAT-STABLE PROTEIN IN MAIZE SEEDLING ROOTS UNDER SALT STRESS

doi:10.17521/cjpe.2005.0068

Abstract

Abstract:

Stress proteins play important roles in plant stress resistance. Phospholipase C/D (PLC/PLD) commonly exists in plants as a kind of signal transfer substance that can induce the expression of some stress proteins. The purpose of our study was to investigate the difference of protein expression between stress treatments and non-stress treatments. Furthermore, the function of PLC/PLD was studied in order to have a deeper insight into the mechanism of PLC/PLD in expression and regulation of stress proteins. Our results showed that a novel heat-stable protein with 69.5 kD-MW in stressed maize (Zea mays) (`LuDan-9002') seedling roots was induced in the 270 mmol·L -1 NaCl treatment and was separated by SDS-PAGE (Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis). The expression content of this protein increased gradually with prolonged treatment of NaCl and still was maintained after the stress treatment was removed. The above results indicate that the 69.5 kD protein may be a kind of stress-adaptable protein. At the same time, a 20 μmol·L -1 ABA treatment could also induce the expression of the 69.5 kD protein, but its expression content was distinctly less than that treated with 270 mmol·L -1 NaCl. In plants treated with NaCl+ABA, the expression content of the 69.5kD protein was less than that treated with NaCl but more than that treated with ABA alone. Neomycin Sulfate (NS) +n-Butyl Alcohol (BA) treatment markedly diminished the expression of the 69.5 kD protein induced by NaCl, ABA or NaCl+ABA, and the inhibition of the two latter treatments strengthened with increasing concentrations of PLC/PLD inhibitors or increased time of treatment, whereas the inhibition of NaCl treatment was more complicated. The results suggest that different treatments have different sensitivities on inducing the expression of the 69.5 kD protein, and the function of PLC/PLD in signal transduction induced by different treatments is somewhat different.

Key words: Maize roots, Salt-stress, Heat-stable protein, PLC/PLD inhibitors, Signal substances

WU Xue-You, SUN Li-Ping, LIU Li-Xia, WU Tao, LI De-Quan. EFFECTS OF PLC/PLD INHIBITOR ON EXPRESSION AND REGULATION OF A NOVEL 69.5 kD HEAT-STABLE PROTEIN IN MAIZE SEEDLING ROOTS UNDER SALT STRESS[J]. Chin J Plant Ecol, 2005, 29(3): 510-515.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2005.0068

https://www.plant-ecology.com/EN/Y2005/V29/I3/510

Figures/Tables 3

Fig.1 The effect of PLC/PLD inhibitors (NS+BA) on the expression content of 69.5 kD protein in maize seedling roots induced by NaCl stress 0: CK 1, 2, 3, 4: 270 mmol·L-1 NaCl treating 48, 72, 96 and R48 h 5, 6, 7: 1 mmol·L-1NS+1 ml·L-1BA+270 mmol·L-1 NaCl treating 48, 72 and 96 h 8, 9, 10:3 mmol·L-1NS+1 ml·L-1BA+270 mmol·L-1 NaCl treating 48, 72 and 96 h

Fig.2 The effect of PLC/PLD inhibitors (NS+BA) on the expression content of 69.5 kD protein in maize seedling roots induced by ABA treatment 0: CK 1, 2, 3, 4: 20 μmol·L -1 ABA treating 48, 72, 96 and R48 h 5, 6, 7: 1 mmol·L-1 NS+1 ml·L-1 BA+20 μmol·L -1 ABA treating 48, 72 and 96 h 8, 9, 10: 3 mmol·L-1NS+1 ml·L-1 BA+20 μmol·L -1 ABA treating 48, 72 and 96 h

Fig.3 The effect of PLC/PLD inhibitors (NS+BA) on the expression content of 69.5 kD protein in maize seedling roots induced by NaCl+ABA treatment 0: CK 1, 2, 3, 4: 270 mmol·L-1 NaCl+20 μmol·L -1ABA treating 48, 72, 96 and R48 h 5, 6, 7: 1 mmol·L-1NS+1 ml·L-1 BA+270 mmol·L-1 NaCl+20 μmol·L -1 ABA treating 48, 72 and 96 h 8, 9, 10: 3 mmol·L-1 NS+1 ml·L-1 BA+270 mmol·L-1 NaCl+20 μmol·L -1 ABA treating 48, 72 and 96 h

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