Chin J Plant Ecol ›› 2020, Vol. 44 ›› Issue (9): 895-904.DOI: 10.17521/cjpe.2020.0197

• Research Articles •     Next Articles

Effects of mating distance and number of pollen donors on sexual reproduction of Sagittaria trifolia

ZHOU Pan-Pan1, CHEN Quan1, ZHANG Yu-Jie2, WANG Zheng-Xiang1,3, DAI Can1,3,*()   

  1. 1 School of Resources and Environmental Science, Hubei University, Wuhan 430062, China
    2 School of Life Sciences, Hubei University, Wuhan 430062, China
    3 Hubei Key Laboratory of Regional Development and Environmental Response (Hubei University), Wuhan 430062, China;
  • Received:2020-06-18 Revised:2020-07-20 Online:2020-09-20 Published:2020-07-27
  • Contact: DAI Can
  • Supported by:
    Supported by the National Natural Science Foundation of China(31670232);the Natural Science Foundation of Hubei(2019CFA066)


Aims Sexual reproduction is the core of plant life history and evolution. Besides the influence of maternal genotype and environment, the contribution of paternal parts on reproduction should not be ignored. In particular, the distance and diversity of pollen donor have significant impacts on fruit setting and offspring quality, which often show distinctive patterns due to specific reproductive characteristics and evolutionary processes of different species or populations. Our objective was to study the effects of mating distance and number of pollen donors on reproductive performance of Sagittaria trifolia.
Methods Three mating distances including selfing, outcrossing of short distance (<50 km), and outcrossing of long distance (>200 km) and two types of pollen donors (single- and double-donor) were investigated in S. trifolia using hand-pollination. Besides selfing, the remaining factors formed a factorial design, resulting in a total of five pollination treatments. We analyzed the differences in fruiting probability, seed number per fruit, seed size, germination rate of F1 seed (year 2018 and 2019) and their seedling length (year 2018 and 2019).
Important findings Different mating distances had no significant effects on fruiting probability, seed number per fruit, seed size, germination rate or seedling length of S. trifolia, implying that there was no inbreeding or outbreeding depression in this species. The increase from single to double pollen donors had no effect on seed production of S. trifolia, but seed germination rate in double-donor treatment was slightly higher than that of single- donor. All together, mating distance and number of donors had little influence on reproductive performance of S. trifolia, which might be related to frequent selfing history, long-distance gene dispersal and high similarity in aquatic habitats of this species. Meanwhile, this study was based on mutual mating designs between genotypes from over 20 natural populations. Such results also imply high and stable reproductive output of S. trifolia at species level.

Key words: mating distance, number of donors, inbreeding depression, outbreeding depression, Sagittaria trifolia