Population genetic diversity and species divergence of Pinus massoniana and P. hwangshanensis at two nucleotide loci

doi:10.3724/SP.J.1258.2011.00531

Abstract

Abstract:

Aims Our objective was to examine population genetic diversity and species divergence of two pine species (Pinus massoniana and P. hwangshanensis) with overlapping distributions in southeastern China.

Methods We estimated levels of nucleotide diversity and an analysis of molecular variance (AMOVA) of the two species based on sequence data of two nuclear loci for 88 individuals from 22 populations of the two species across most of their range of distribution. Gene genealogies of each locus were constructed by coalescent simulations using the Median-Joining model.

Important findings Both species exhibit low levels of nucleotide diversity at two nucleotide loci, and the level of silent nucleotide diversity is two times higher in P. hwangshanensis (π_sil= 0.003 40) than that in P. massoniana (π_sil= 0.001 71). The population differentiation (F_ST) is also significantly different between the two species (P. massoniana, 0.059; P. hwangshanensis, 0.339, p < 0.05). These genetic differences in the population structure of the two species may result from their differences in distribution and habitat preference. Hierarchical AMOVA revealed that the average of variance components between species is 48.86% based on the two loci and is signiﬁcantly higher at locus GI (77.24%) than locus C3H (20.48%). In addition, shared haplotypes were only observed in C3H rather than GI. Thus we speculate that GI (control of flowering time) may have experienced speciation-related selection, which further accelerated its lineage-sorting divergence between the species.

Key words: nucleotide diversity, Pinus hwangshanensis, P. massoniana, population genetics structure, species divergence

ZHANG Li-Rui, PENG Yan-Ling, REN Guang-Peng, ZHOU Yong-Feng, LI Zhong-Hu, LIU Jian-Quan. Population genetic diversity and species divergence of Pinus massoniana and P. hwangshanensis at two nucleotide loci[J]. Chin J Plant Ecol, 2011, 35(5): 531-538.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00531

https://www.plant-ecology.com/EN/Y2011/V35/I5/531

Figures/Tables 4

Fig. 1 Sampling localities of Pinus massoniana and P. hwangshanensis (A) and gene genealogies of nuclear loci C3H and GI (B, C).

Table 1 Description of the C3H and GI locus

Table 2 Nucleotide diversity and neutrality tests over the C3H and GI locus in Pinus massoniana and P. hwangshanensis

Table 3 Analysis of molecular variance (AMOVA) of nuclear loci C3H and GI variation in Pinus massoniana and P. hwangshanensis (10 000 permutations)

	变异来源 Source of variation (%)			遗传分化系数 Coefficient of gene differentiation (F_ST)
基因座位 Gene locus	种间 Among species	种内群体间 Among populations within species	群体内 Within population	马尾松 P. massoniana	黄山松 P. hwangshanensis
C3H	20.48	15.81	63.71	0.071	0.210^*
GI	77.24	6.99	15.77	0.047	0.467^**
平均值 Average	48.86	11.35	39.74	0.059	0.339

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