A preliminary study of molecular phylogeny and biogeography distribution pattern of Zelkova inferred from trnL-trnF and nrITS sequences

doi:10.3724/SP.J.1258.2013.00042

Abstract

Abstract:

Aims Zelkova, belongs to the Ulmaceae, consists of only six species and has a disjunct distribution in East Asia, West Asia and South Europe. Molecular phylogenetic analysis and dispersal and vicariance analysis (DIVA) were employed to analyze phylogenetic relationships and biogeographic pattern of Zelkova.
Methods The phylogenetic tree was reconstructed based on DNA sequences of trnL-trnF and ITS regions. The analysis of DIVA was used to deduce the biogeographic pattern.
Important findings Results showed that three phylogenetic clades exist in Zelkova, and they correspond to groups from East Asia, West Asia and South Europe. The present phylogenetic tree is different from that in previous study based on nrITS region. DIVA analyses of this genus indicated that the ancestral area of Zelkova was a larger area including East Asia, West Asia and South Europe. The speciation process was dominant by vicariance. Each of the three areas was gradually separated from vicariant events. Based on the high level of species diversity of Zelkova in East Asia, the center of origin is probably the northern Pacific, i.e., north of China and Japan. The current distribution pattern of this genus was likely shaped by historical geological and environmental events, such as the retreat of Tethys, the Qinghai-Xizang Plateau uplift and the Quaternary glaciations that shrank distribution areas.

Key words: biogeography distribution pattern, molecular phylogeny, Ulmaceae, Zelkova

WANG Lu,LEI Yun,ZHANG Ming-Li. A preliminary study of molecular phylogeny and biogeography distribution pattern of Zelkova inferred from trnL-trnF and nrITS sequences[J]. Chin J Plant Ecol, 2013, 37(5): 407-414.

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Figures/Tables 4

References 41

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物种 Species	来源 Voucher\Source	Gen-Bank
物种 Species	来源 Voucher\Source	ITS	trnL-trnF
Zelkova abelicea 1	希腊克里特岛 Crete, Greece, Heinrich and Wagner, 378 (SZU)	KC843951	KC843966
Z. carpinifolia 1	德国柏林植物园 Berlin Botanical Garden, Berlin, Germany, 064-92-74-80	KC843945	KC843960
Z. carpinifolia 2	德国美茵兹大学植物园 Mainz University Botanical Garden, Mainz, Germany, 506\1	KC843946	KC843962
大叶榉树1 Z. schneideriana 1	德国柏林植物园 Berlin Botanical Garden, Berlin, Germany, 033-06-89-70	AJ622865.1	KC843959
大叶榉树2 Z. schneideriana 2	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	-	KC843965
榉树1 Z. serrata 1	重庆市巫溪县 Wuxi, Chongqing, 陈彦生等, 1473; Chen et al., 1473	KC843947	KC843958
榉树2 Z. serrata 2	德国美茵兹大学植物园 Mainz University Botanical Garden, Mainz, Germany, 621\1	KC843949	KC843961
榉树3 Z. serrata 3	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843950	KC843964
榉树4 Z. serrata 4	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843948
Z. sicula 1	意大利西西里岛 Sicily, Italy, A. TROIA, 08\7\2002	AJ622883.1	-
大果榉1 Z. sinica 1	中国科学院武汉植物园 Wuhan Botanical Garden, CAS	-	KC843963
外类群 Outside species
大叶朴 Celtis koraiensis	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843956	KC843967
刺榆 Hemiptelea davidii	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843955	KC843969
刺榆2 H. davidii 2	-	AJ622834.1	-
青檀 Pteroceltis tatarinowii	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843957	KC843968
青檀2 P. tatarinowii 2	-	-	AF501597.1
美洲榆 Ulmus americana	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843954	KC843971
美洲榆2 U. americana 2	-	-	AF501593.1
多脉榆 U. castaneifolia	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	-	KC843970
脱皮榆 U. lamellosa	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843953	KC843973
榆树 U. pumila	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843952	KC843972

物种 Species	来源 Voucher\Source	Gen-Bank
物种 Species	来源 Voucher\Source	ITS	trnL-trnF
Zelkova abelicea 1	希腊克里特岛 Crete, Greece, Heinrich and Wagner, 378 (SZU)	KC843951	KC843966
Z. carpinifolia 1	德国柏林植物园 Berlin Botanical Garden, Berlin, Germany, 064-92-74-80	KC843945	KC843960
Z. carpinifolia 2	德国美茵兹大学植物园 Mainz University Botanical Garden, Mainz, Germany, 506\1	KC843946	KC843962
大叶榉树1 Z. schneideriana 1	德国柏林植物园 Berlin Botanical Garden, Berlin, Germany, 033-06-89-70	AJ622865.1	KC843959
大叶榉树2 Z. schneideriana 2	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	-	KC843965
榉树1 Z. serrata 1	重庆市巫溪县 Wuxi, Chongqing, 陈彦生等, 1473; Chen et al., 1473	KC843947	KC843958
榉树2 Z. serrata 2	德国美茵兹大学植物园 Mainz University Botanical Garden, Mainz, Germany, 621\1	KC843949	KC843961
榉树3 Z. serrata 3	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843950	KC843964
榉树4 Z. serrata 4	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843948
Z. sicula 1	意大利西西里岛 Sicily, Italy, A. TROIA, 08\7\2002	AJ622883.1	-
大果榉1 Z. sinica 1	中国科学院武汉植物园 Wuhan Botanical Garden, CAS	-	KC843963
外类群 Outside species
大叶朴 Celtis koraiensis	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843956	KC843967
刺榆 Hemiptelea davidii	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843955	KC843969
刺榆2 H. davidii 2	-	AJ622834.1	-
青檀 Pteroceltis tatarinowii	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843957	KC843968
青檀2 P. tatarinowii 2	-	-	AF501597.1
美洲榆 Ulmus americana	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843954	KC843971
美洲榆2 U. americana 2	-	-	AF501593.1
多脉榆 U. castaneifolia	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	-	KC843970
脱皮榆 U. lamellosa	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843953	KC843973
榆树 U. pumila	中国科学院北京植物园 Beijing Botanical Garden, Institute of Botany, CAS	KC843952	KC843972