TIMING AND CONCENTRATION EFFECTS ON THE DEFENSE RESPONSE OF ZEA MAYS SEEDLINGS AFTER APPLICATION OF JASMONIC ACID TO LEAVES

doi:10.3773/j.issn.1005-264x.2009.04.020

Abstract

Abstract:

Aims Our objectives were to investigate temporal dynamics of the contents of direct defense chemicals (DIMBOA and total phenolics) and the expression levels of their corresponding key genes (Bx1, Bx9 and PAL), direct defense protein-related genes (PR-1, PR-2a and MPI), and indirect defense chemical volatile-related genes (FPS and TPS) in leaves and root systems of Zea mays seedlings under the application of exogenous jasmonic acid (JA) on leaf surface.
Methods Leaves of Z. mays cultivar Gaoyou 115 were treated with JA with concentrations of 1, 2.5, 5 and 10 mmol·L^-1. We examined contents of the defense chemicals and expression dynamics of their synjournal-mediated genes, direct defense protein-related genes and indirect defense chemical volatile-related genes in both leaves and roots from 3 to 48 h after the application.
Important findings Exogenous JA application resulted in significant timing and concentration effects on the chemical defense response. Gene expression of Bx9 and PAL in the leaves could be induced 3 to 6 h after treatment, resulting in an apparent increase in the content of DIMBOA and total phenolics. The increase was positively correlated with JA concentrations, but the inductive effect gradually decreased afterward. Gene expression of PR-2a and MPI in the leaves was also dramatically induced by JA up to 24 and 48 h, respectively. High JA concentrations induced the expression of FPS gene, whereas low JA concentrations had inducible effects on the expression of TPS after 3-6 h. In addition, JA treatment to leaves could indirectly induce the defense response in roots. Most parameters measured in the indirect induction mainly occurred in the later phase (after 24-48 h) of the treatment. For example, JA systematically increased the contents of DIMBOA and total phenolics in the roots and enhanced the expression of PR-2a, MPI, FPS and TPS genes in the roots 48 h after the treatment, showing a tendency of positive relationship with JA concentrations. These findings provided evidence that the indirect induction by JA application to Z. mays leaves on roots was not as strong as on leaves. Leaves started the defense response earlier than roots, and the induction of defense response both in leaves and roots was increased with increasing JA concentrations.

Key words: Zea mays, jasmonic acid, induced defense, timing and concentration effects

FENG Yuan-Jiao, WANG Jian-Wu, LUO Shi-Ming. TIMING AND CONCENTRATION EFFECTS ON THE DEFENSE RESPONSE OF ZEA MAYS SEEDLINGS AFTER APPLICATION OF JASMONIC ACID TO LEAVES[J]. Chin J Plant Ecol, 2009, 33(4): 812-823.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.04.020

https://www.plant-ecology.com/EN/Y2009/V33/I4/812

Figures/Tables 8

Table 1 The specific primers used in the experiment

基因 Genes	登陆号 Accession number	引物 Primers	大小 Size (bp)
吲哚合成酶 Indole synthase (Bx1)	AY254103	F: 5°-ATGGCTTTCGCGCCCAAAACGTCCTC-3° R: 5°-CGTGGACCCCCGCCTCTTTCATCTCG-3°	612
糖基转移酶 Glucosyltransferase (Bx9)	AF331885	F: 5°-TCGTCACCACGCTGAACGCCAG-3° R: 5°-GGATCCTCCTTGCGCTCCTCTTTC-3°	262
苯丙氨酸转氨酶 Phenylalanine ammonia-lyase (PAL)	L77912	F: 5°-CACAAG CTGAAGCACCACCC-3° R: 5°-GAGTTCACGTCCTGGTTGTG-3°	560
病程相关蛋白-1 Pathogenesis related protein-1 (PR-1)	U82200	F: 5°-GTGGACCCGCACAACGCG-3° R: 5°-GCCGATGGCGGTGGAGTC-3°	309
酸性β-1,3-葡聚糖酶 Acidic beta-1,3-glucanase (PR-2 a)	M95407	F: 5°-CCAACGTCTACCCCTACTTC-3° R: 5°-GGGTTGAAGAGGCCGAAGTG-3°	394
玉米蛋白酶抑制剂 Maize proteinase inhibitor (MPI)	X78988	F: 5°-ACAACCAGCAGTGCAACAAG-3° R: 5°-GAAGATGCGGACACGGTTAG-3°	370
法呢烯基焦磷酸合成酶 Farnesyl pyrophosphate synthetase (FPS)	L39789	F: 5°-GGCTGGTGCATTGAATGGCT-3° R: 5°-ATGTCCGTTCCAATCTTGCC-3°	518
萜类合成酶 Terpene synthase (TPS)	AF529266	F: 5°-GCCATGCCAGTGAAGCTGACTCCTGC-3° R: 5°-GTAGACGGTCCAATGTGGTGTAGAAG-3°	679
甘油醛-3-磷酸脱氢酶 Glyceraldehyde-3-phosphate dehydrogenase (GAPc)	X07156	F: 5°-GCTAGCTGCACCACAAACTGCCT-3° R: 5°-TAGCCCCACTCGTTGTCGTACCA-3°	512

Fig. 1 Effects of different concentrations of jasmonic acid to maize leaves on the content of DIMBOA in leaves and roots at different time intervals

Fig. 2 Effects of different concentrations of jasmonic acid to maize leaves on the expression patterns of key genes in DIMBOA biosynjournal in leaves and roots at different time intervals

Fig. 3 Effects of different concentrations of jasmonic acid to maize leaves on the content of total phenolics in leaves and roots at different time intervals

Fig. 4 Effects of different concentrations of jasmonic acid to maize leaves on the expression patterns of key genes in total phenolics biosynjournal in leaves and roots at different time intervals

Fig. 5 Effects of different concentrations of jasmonic acid to maize leaves on the expression patterns of pathogenesis related protein genes in leaves and roots at different time intervals

Fig. 6 Effects of different concentrations of jasmonic acid to maize leaves on the expression patterns of proteinase inhibitor gene in leaves and roots at different time intervals

Fig. 7 Effects of different concentrations of jasmonic acid to maize leaves on the expression patterns of key genes in terpenoids biosynjournal in leaves and roots at different time intervals

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