Chin J Plant Ecol ›› 2011, Vol. 35 ›› Issue (9): 937-945.DOI: 10.3724/SP.J.1258.2011.00937
• Research Articles • Previous Articles Next Articles
TAN Xiao-Mei1,2(), ZHOU Zhi-Chun1,**(
), JIN Guo-Qing1, ZHANG Yi1
Received:
2011-03-08
Accepted:
2011-07-22
Online:
2011-03-08
Published:
2011-09-01
Contact:
ZHOU Zhi-Chun
TAN Xiao-Mei, ZHOU Zhi-Chun, JIN Guo-Qing, ZHANG Yi. Paternity analysis and pollen dispersal for the second generation clonal seed orchard of Pinus massoniana[J]. Chin J Plant Ecol, 2011, 35(9): 937-945.
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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00937
位点 Locus | 来源 Source | 引物序列(5′-3′) Primer sequences (5′-3′) | 重复单位 Repeat units | 参考文献 Reference |
---|---|---|---|---|
PTest1 | 火炬松 Pinus taeda | F)CGATGTCGATTAGGGATTGG R)CCTGTTCTTCGTCGGATGTT | (GGA)6 | |
PtTX4001 | 火炬松 P. taeda | F)CTATTTGAGTTAAGAAGGGAGTC R)CTGTGGGTAGCATCATC | (GT)5 | |
RPTest11 | 火炬松 P. taeda | F)AGGATGCCTATGATATGCGC R)AACCATAACAAAAGCGGTCG | (CAT)7 | |
PtTX2123 | 火炬松 P. taeda | F)AAGAACCCACAAACACAAG R)GGGCAAGAATTCAATGATAA | (AGC)8 | |
PtTX3116 | 火炬松 P. taeda | F)CCTCCCAAAGCCTAAAGAAT R)CATACAAGGCCTTATCTTACAGAA | (TTG)7…(TTG)5 | |
RPS160 | 北美乔松 P. strobus | F)ACTAAGAACTCTCCCTCTCACC R)TCATTGTTCCCCAAATCAT | (ACAG)3AGGC(ACAC)3 | |
PtTX4062 | 火炬松 P. taeda | F)TCTAGGCAATCTTTTTACCAAC R)ATCATAGCCTCATCCAATACA | (T)8(GT)13 | |
PR203 | 辐射松 P. radiata | F)TGGGACCCCATATTCTGATG R)CATTCCACTAGTTCTCTCGCAC | (GA)14 | |
PR4.6 | 辐射松 P. radiata | F)GAAAAAAAGGCAAAAAAAAGGAG R)ACCCAAGGCTACATAACTCG | (CA)21(TA)6 | |
PtTX4084 | 火炬松 P. taeda | F)ACTGGCGAAGGCGAGCCGACAC R)ACCGCTGTGGGAACCCTCCTCGTC | (GCT)8 | |
Cjgssr170 | 日本柳杉 Cryptomeria japonica | F)TGGCGATGTGGTTCTTATGG R)CAACCGCATCTTTCCCTAATTC | (CT)33 |
Table 1 The sequence of SSR primers
位点 Locus | 来源 Source | 引物序列(5′-3′) Primer sequences (5′-3′) | 重复单位 Repeat units | 参考文献 Reference |
---|---|---|---|---|
PTest1 | 火炬松 Pinus taeda | F)CGATGTCGATTAGGGATTGG R)CCTGTTCTTCGTCGGATGTT | (GGA)6 | |
PtTX4001 | 火炬松 P. taeda | F)CTATTTGAGTTAAGAAGGGAGTC R)CTGTGGGTAGCATCATC | (GT)5 | |
RPTest11 | 火炬松 P. taeda | F)AGGATGCCTATGATATGCGC R)AACCATAACAAAAGCGGTCG | (CAT)7 | |
PtTX2123 | 火炬松 P. taeda | F)AAGAACCCACAAACACAAG R)GGGCAAGAATTCAATGATAA | (AGC)8 | |
PtTX3116 | 火炬松 P. taeda | F)CCTCCCAAAGCCTAAAGAAT R)CATACAAGGCCTTATCTTACAGAA | (TTG)7…(TTG)5 | |
RPS160 | 北美乔松 P. strobus | F)ACTAAGAACTCTCCCTCTCACC R)TCATTGTTCCCCAAATCAT | (ACAG)3AGGC(ACAC)3 | |
PtTX4062 | 火炬松 P. taeda | F)TCTAGGCAATCTTTTTACCAAC R)ATCATAGCCTCATCCAATACA | (T)8(GT)13 | |
PR203 | 辐射松 P. radiata | F)TGGGACCCCATATTCTGATG R)CATTCCACTAGTTCTCTCGCAC | (GA)14 | |
PR4.6 | 辐射松 P. radiata | F)GAAAAAAAGGCAAAAAAAAGGAG R)ACCCAAGGCTACATAACTCG | (CA)21(TA)6 | |
PtTX4084 | 火炬松 P. taeda | F)ACTGGCGAAGGCGAGCCGACAC R)ACCGCTGTGGGAACCCTCCTCGTC | (GCT)8 | |
Cjgssr170 | 日本柳杉 Cryptomeria japonica | F)TGGCGATGTGGTTCTTATGG R)CAACCGCATCTTTCCCTAATTC | (CT)33 |
位点 Locus | 等位基因数目 Number of alleles | 观测杂合度 Observed heterozygosity | 期望杂合度 Expected heterozygosity | 多态性信息含量 Polymorphic information content |
---|---|---|---|---|
PTest1 | 4 | 0.462 | 0.500 | 0.377 |
PtTX4001 | 6 | 0.291 | 0.321 | 0.292 |
RPTest11 | 2 | 0.467 | 0.359 | 0.294 |
PtTX2123 | 4 | 0.554 | 0.519 | 0.430 |
PtTX3116 | 9 | 0.758 | 0.841 | 0.820 |
RPS160 | 4 | 0.318 | 0.281 | 0.253 |
PtTX4062 | 4 | 0.272 | 0.236 | 0.209 |
PR203 | 8 | 0.568 | 0.691 | 0.637 |
PR4.6 | 11 | 0.598 | 0.605 | 0.563 |
PtTX4084 | 4 | 0.133 | 0.125 | 0.118 |
Cjgssr170 | 5 | 0.288 | 0.280 | 0.261 |
平均值 Mean | 5.55 | 0.428 | 0.433 | 0.387 |
Table 2 Polymorphism of SSR in Pinus massoniana
位点 Locus | 等位基因数目 Number of alleles | 观测杂合度 Observed heterozygosity | 期望杂合度 Expected heterozygosity | 多态性信息含量 Polymorphic information content |
---|---|---|---|---|
PTest1 | 4 | 0.462 | 0.500 | 0.377 |
PtTX4001 | 6 | 0.291 | 0.321 | 0.292 |
RPTest11 | 2 | 0.467 | 0.359 | 0.294 |
PtTX2123 | 4 | 0.554 | 0.519 | 0.430 |
PtTX3116 | 9 | 0.758 | 0.841 | 0.820 |
RPS160 | 4 | 0.318 | 0.281 | 0.253 |
PtTX4062 | 4 | 0.272 | 0.236 | 0.209 |
PR203 | 8 | 0.568 | 0.691 | 0.637 |
PR4.6 | 11 | 0.598 | 0.605 | 0.563 |
PtTX4084 | 4 | 0.133 | 0.125 | 0.118 |
Cjgssr170 | 5 | 0.288 | 0.280 | 0.261 |
平均值 Mean | 5.55 | 0.428 | 0.433 | 0.387 |
父本 Male parent | 交配母本 Female parent mated | 子代数Numbers of progeny | 繁殖贡献率Reproductive success (%) | 父本 Male parent | 交配母本 Female parent mated | 子代数Numbers of progeny | 繁殖贡献率Reproductive success (%) |
---|---|---|---|---|---|---|---|
1 | 31, 39 | 3 | 1.29 | 32 | 9, 19, 31, 52 | 6 | 2.59 |
2 | 19, 52 | 3 | 1.29 | 37 | 16, 39, 51, 52 | 6 | 2.59 |
3 | 31 | 2 | 0.86 | 38 | 39, 51 | 3 | 1.29 |
6 | 39, 51 | 3 | 1.29 | 39 | 16, 39, 51, 52 | 5 | 2.16 |
7 | 39, 51, 53 | 3 | 1.29 | 43 | 39, 52 | 2 | 0.86 |
11 | 16, 19 | 2 | 0.86 | 44 | 16, 31 | 5 | 2.16 |
12 | 9, 31, 39, 51, 53 | 9 | 3.88 | 48 | 16, 51, 52 | 3 | 1.29 |
13 | 16 | 2 | 0.86 | 49 | 16, 19, 52 | 5 | 2.16 |
14 | 9, 19, 31, 51 | 7 | 3.02 | 50 | 53 | 1 | 0.43 |
15 | 19, 39, 51 | 7 | 3.02 | 51 | 19 | 1 | 0.43 |
16 | 16, 19, 51, 52, 53 | 7 | 3.02 | 52 | 51 | 1 | 0.43 |
17 | 31, 51, 53 | 4 | 1.72 | 53 | 19 | 1 | 0.43 |
20 | 19, 52 | 2 | 0.86 | 54 | 9, 39, 51, 53 | 7 | 3.02 |
21 | 31, 53 | 2 | 0.86 | 55 | 9, 31, 39, 52 | 5 | 2.16 |
23 | 16, 19, 39, 51, 52 | 9 | 3.88 | 57 | 9, 16, 53 | 3 | 1.29 |
25 | 16, 19, 31, 52, 53 | 6 | 2.59 | 60 | 16, 19, 39 | 5 | 2.16 |
26 | 16, 39, 53 | 4 | 1.72 | 61 | 16, 39, 51, 53 | 12 | 5.17 |
28 | 9, 52, 53 | 3 | 1.29 | 63 | 19, 53 | 5 | 2.16 |
29 | 19, 31 | 4 | 1.72 | 66 | 16, 31, 52, 53 | 5 | 2.16 |
30 | 9, 16, 31, 51, 52, 53 | 8 | 3.45 | 62 | 9, 19, 39, 51, 53 | 6 | 2.59 |
31 | 9, 16, 31, 51, 53 | 7 | 3.02 | 88 | 16, 19, 39, 53 | 4 | 1.72 |
19 | 9, 16, 31, 39, 51, 52, 53 | 11 | 4.74 | 45 | 9, 16, 19, 31, 39, 53 | 8 | 3.45 |
47 | 16, 19, 31, 52, 53 | 10 | 4.31 | 58 | 9, 16, 19, 39, 52, 53 | 15 | 6.47 |
Table 3 Paternity analysis and male reproductive success of the second generation clonal seed orchard of Pinus massoniana
父本 Male parent | 交配母本 Female parent mated | 子代数Numbers of progeny | 繁殖贡献率Reproductive success (%) | 父本 Male parent | 交配母本 Female parent mated | 子代数Numbers of progeny | 繁殖贡献率Reproductive success (%) |
---|---|---|---|---|---|---|---|
1 | 31, 39 | 3 | 1.29 | 32 | 9, 19, 31, 52 | 6 | 2.59 |
2 | 19, 52 | 3 | 1.29 | 37 | 16, 39, 51, 52 | 6 | 2.59 |
3 | 31 | 2 | 0.86 | 38 | 39, 51 | 3 | 1.29 |
6 | 39, 51 | 3 | 1.29 | 39 | 16, 39, 51, 52 | 5 | 2.16 |
7 | 39, 51, 53 | 3 | 1.29 | 43 | 39, 52 | 2 | 0.86 |
11 | 16, 19 | 2 | 0.86 | 44 | 16, 31 | 5 | 2.16 |
12 | 9, 31, 39, 51, 53 | 9 | 3.88 | 48 | 16, 51, 52 | 3 | 1.29 |
13 | 16 | 2 | 0.86 | 49 | 16, 19, 52 | 5 | 2.16 |
14 | 9, 19, 31, 51 | 7 | 3.02 | 50 | 53 | 1 | 0.43 |
15 | 19, 39, 51 | 7 | 3.02 | 51 | 19 | 1 | 0.43 |
16 | 16, 19, 51, 52, 53 | 7 | 3.02 | 52 | 51 | 1 | 0.43 |
17 | 31, 51, 53 | 4 | 1.72 | 53 | 19 | 1 | 0.43 |
20 | 19, 52 | 2 | 0.86 | 54 | 9, 39, 51, 53 | 7 | 3.02 |
21 | 31, 53 | 2 | 0.86 | 55 | 9, 31, 39, 52 | 5 | 2.16 |
23 | 16, 19, 39, 51, 52 | 9 | 3.88 | 57 | 9, 16, 53 | 3 | 1.29 |
25 | 16, 19, 31, 52, 53 | 6 | 2.59 | 60 | 16, 19, 39 | 5 | 2.16 |
26 | 16, 39, 53 | 4 | 1.72 | 61 | 16, 39, 51, 53 | 12 | 5.17 |
28 | 9, 52, 53 | 3 | 1.29 | 63 | 19, 53 | 5 | 2.16 |
29 | 19, 31 | 4 | 1.72 | 66 | 16, 31, 52, 53 | 5 | 2.16 |
30 | 9, 16, 31, 51, 52, 53 | 8 | 3.45 | 62 | 9, 19, 39, 51, 53 | 6 | 2.59 |
31 | 9, 16, 31, 51, 53 | 7 | 3.02 | 88 | 16, 19, 39, 53 | 4 | 1.72 |
19 | 9, 16, 31, 39, 51, 52, 53 | 11 | 4.74 | 45 | 9, 16, 19, 31, 39, 53 | 8 | 3.45 |
47 | 16, 19, 31, 52, 53 | 10 | 4.31 | 58 | 9, 16, 19, 39, 52, 53 | 15 | 6.47 |
项目 Item | 母树 Maternity | ||||||
---|---|---|---|---|---|---|---|
16 | 19 | 31 | 39 | 51 | 52 | 53 | |
树冠南面父本数 Male numbers in the south of crown | 14 | 12 | 9 | 13 | 12 | 9 | 13 |
树冠北面父本数 Male numbers in the north of crown | 15 | 11 | 12 | 9 | 12 | 13 | 11 |
树冠南面平均交配距离 Average mating distance in the south of crown (m) | 74 | 124 | 54 | 41 | 73 | 49 | 40 |
树冠北面平均交配距离 Average mating distance in the north of crown (m) | 64 | 86 | 42 | 28 | 66 | 51 | 39 |
树冠南面确定父本的子代数 Assigning paternity progenies in the south of crown | 16 | 16 | 13 | 18 | 14 | 12 | 16 |
树冠北面确定父本的子代数 Assigning paternity progenies in the north of crown | 19 | 14 | 16 | 16 | 14 | 15 | 15 |
Table 4 Paternity constitution and parents mating distance of progeny in the south and north of crown
项目 Item | 母树 Maternity | ||||||
---|---|---|---|---|---|---|---|
16 | 19 | 31 | 39 | 51 | 52 | 53 | |
树冠南面父本数 Male numbers in the south of crown | 14 | 12 | 9 | 13 | 12 | 9 | 13 |
树冠北面父本数 Male numbers in the north of crown | 15 | 11 | 12 | 9 | 12 | 13 | 11 |
树冠南面平均交配距离 Average mating distance in the south of crown (m) | 74 | 124 | 54 | 41 | 73 | 49 | 40 |
树冠北面平均交配距离 Average mating distance in the north of crown (m) | 64 | 86 | 42 | 28 | 66 | 51 | 39 |
树冠南面确定父本的子代数 Assigning paternity progenies in the south of crown | 16 | 16 | 13 | 18 | 14 | 12 | 16 |
树冠北面确定父本的子代数 Assigning paternity progenies in the north of crown | 19 | 14 | 16 | 16 | 14 | 15 | 15 |
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