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GENETIC DIVERSITY IN FRAGMENTED POPULATIONS OF BERCHEMIELLA WILSONII VAR. PUBIPETIOLATA, AN ENDANGERED PLANT ENDEMIC TO EASTERN CHINA
Received date: 2004-12-28
Accepted date: 2005-04-10
Online published: 2006-01-30
Berchemiella wilsonii var. pubipetiolata (Rhamnaceae) is an endangered tree species with a narrow distribution in fragmented habitat patches in Tianmu Mountain, Zhejiang Province and Dabie Mountain, Anhui Province, east of China. The habitat has been severely disturbed because of agricultural practices by local residents. Over the past several decades, there has been a decrease in the population number and size. Information on genetic variation and structure is critical for developing successful conservation strategies for this species. The amplified fragment length polymorphisms (AFLPs) were used to evaluate the genetic diversity and population structure of four extant populations of B. wilsonii var. pubipetiolata. A total of 122 unambiguous bands were generated using eight primer combinations of which 45 (36.9%) were polymorphic across all individuals. In comparison with other endangered woody plants, B. wilsonii var. pubipetiolata has similar genetic diversity at the population level (Pp=26.4%, Hep=0.162 8) and species level (Ps=36.9%, Hes=0.202 4). A relatively high value of FST (0.193 9) indicated significant genetic differentiation among populations. Analysis of molecular variance (AMOVA) further revealed that 24.88% of the AFLP variation resided between geographic domes (Zhejiang and Anhui Province), 14.71% among populations and 60.42% within populations. The results of this study suggest that habitat fragmentation and reduction of population size caused by anthropogenic activities have caused genetic erosion and population differentiation in B. wilsonii var. pubipetiolata, which poses a potential threat to the long-term survivability of this species. Genetic information obtained in the present study provides useful baseline data for formulating conservation strategies. Conservation management should include both in situ conservation and ex situ conservation programs and need to be well designed. An ex situ conservation strategy for extensive sampling of all extant populations to maximize genomic representation of the species is recommended.
XU Feng-Hua, KANG Ming, HUANG Hong-Wen, JIANG Ming-Xi . GENETIC DIVERSITY IN FRAGMENTED POPULATIONS OF BERCHEMIELLA WILSONII VAR. PUBIPETIOLATA, AN ENDANGERED PLANT ENDEMIC TO EASTERN CHINA[J]. Chinese Journal of Plant Ecology, 2006 , 30(1) : 157 -164 . DOI: 10.17521/cjpe.2006.0022
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