Chin J Plant Ecol ›› 2006, Vol. 30 ›› Issue (1): 157-164.DOI: 10.17521/cjpe.2006.0022
Special Issue: 生物多样性
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XU Feng-Hua, KANG Ming, HUANG Hong-Wen*(), JIANG Ming-Xi
Received:
2004-12-28
Accepted:
2005-04-10
Online:
2006-12-28
Published:
2006-01-30
Contact:
HUANG Hong-Wen
XU Feng-Hua, KANG Ming, HUANG Hong-Wen, JIANG Ming-Xi. GENETIC DIVERSITY IN FRAGMENTED POPULATIONS OF BERCHEMIELLA WILSONII VAR. PUBIPETIOLATA, AN ENDANGERED PLANT ENDEMIC TO EASTERN CHINA[J]. Chin J Plant Ecol, 2006, 30(1): 157-164.
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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2006.0022
居群 Population | 取样数(N) No. of sampling | 多态位点百分率(P) Polymorphism(%) | 基因多样度(He) Gene diversity | 居群特有带 Population specific bands | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
湍口Tuankou | 23 | 23.8 | 0.140 5 (0.015 12) | 0 | |||||||||
马啸Maxiao | 13 | 26.2 | 0.171 4 (0.014 87) | 0 | |||||||||
马家河Majiahe | 31 | 29.5 | 0.166 8 (0.015 38) | 2 | |||||||||
万佛山Wanfoshan | 22 | 26.2 | 0.172 4 (0.014 66) | 1 | |||||||||
居群平均Population | 22.25 | 26.4 | 0.162 8 (0.007 52) | ||||||||||
物种水平Species | 89 | 36.9 | 0.202 4 |
Table 1 Sampling sites and genetic diversity of Berchemiella wilsonii var. pubipetiolata
居群 Population | 取样数(N) No. of sampling | 多态位点百分率(P) Polymorphism(%) | 基因多样度(He) Gene diversity | 居群特有带 Population specific bands | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
湍口Tuankou | 23 | 23.8 | 0.140 5 (0.015 12) | 0 | |||||||||
马啸Maxiao | 13 | 26.2 | 0.171 4 (0.014 87) | 0 | |||||||||
马家河Majiahe | 31 | 29.5 | 0.166 8 (0.015 38) | 2 | |||||||||
万佛山Wanfoshan | 22 | 26.2 | 0.172 4 (0.014 66) | 1 | |||||||||
居群平均Population | 22.25 | 26.4 | 0.162 8 (0.007 52) | ||||||||||
物种水平Species | 89 | 36.9 | 0.202 4 |
引物组合 Primer combination | 引物序列 Primer sequence (5'-3') | 总位点数 Total bands | 多态位点数 Polymorphic bands | 多态位点百分率 Polymorphism(%) |
---|---|---|---|---|
EAAC/MCGT | GACTGCGTACCAATTCAAC | 16 | 8 | 50.0 |
GATGAGTCCTGAGTAACGT | ||||
EACT/MCAT | GACTGCGTACCAATTCACT | 15 | 7 | 46.7 |
GATGAGTCCTGAGTAACAT | ||||
EATC/MCAA | GACTGCGTACCAATTCATC | 13 | 3 | 23.1 |
GATGAGTCCTGAGTAACAA | ||||
EAAG/MCGA | GACTGCGTACCAATTCAAG | 20 | 9 | 45.0 |
GATGAGTCCTGAGTAACGA | ||||
EAAG/MCGT | GACTGCGTACCAATTCAAG | 17 | 4 | 23.5 |
GATGAGTCCTGAGTAACGT | ||||
EAAG/MCAT | GACTGCGTACCAATTCAAG | 15 | 6 | 40.0 |
GATGAGTCCTGAGTAACAT | ||||
EACA/MCAA | GACTGCGTACCAATTCACA | 12 | 7 | 58.3 |
GATGAGTCCTGAGTAACAA | ||||
EACA/MCAT | GACTGCGTACCAATTCACA | 14 | 1 | 7.1 |
GATGAGTCCTGAGTAACAT | ||||
总计Total | 122 | 45 | 36.9 |
Table 2 Primer combinations and primer sequences used in AFLP analysis; and AFLPs generated among 89 individuals of Berchemiella wilsonii var. pubipetiolata using the eight combinations
引物组合 Primer combination | 引物序列 Primer sequence (5'-3') | 总位点数 Total bands | 多态位点数 Polymorphic bands | 多态位点百分率 Polymorphism(%) |
---|---|---|---|---|
EAAC/MCGT | GACTGCGTACCAATTCAAC | 16 | 8 | 50.0 |
GATGAGTCCTGAGTAACGT | ||||
EACT/MCAT | GACTGCGTACCAATTCACT | 15 | 7 | 46.7 |
GATGAGTCCTGAGTAACAT | ||||
EATC/MCAA | GACTGCGTACCAATTCATC | 13 | 3 | 23.1 |
GATGAGTCCTGAGTAACAA | ||||
EAAG/MCGA | GACTGCGTACCAATTCAAG | 20 | 9 | 45.0 |
GATGAGTCCTGAGTAACGA | ||||
EAAG/MCGT | GACTGCGTACCAATTCAAG | 17 | 4 | 23.5 |
GATGAGTCCTGAGTAACGT | ||||
EAAG/MCAT | GACTGCGTACCAATTCAAG | 15 | 6 | 40.0 |
GATGAGTCCTGAGTAACAT | ||||
EACA/MCAA | GACTGCGTACCAATTCACA | 12 | 7 | 58.3 |
GATGAGTCCTGAGTAACAA | ||||
EACA/MCAT | GACTGCGTACCAATTCACA | 14 | 1 | 7.1 |
GATGAGTCCTGAGTAACAT | ||||
总计Total | 122 | 45 | 36.9 |
居群 Population | 湍口 Tuankou | 马啸 Maxiao | 马家河 Majiahe | 万佛山 Wanfoshan |
---|---|---|---|---|
湍口Tuankou | - | 1.982 | 0.588 | 0.643 |
马啸Maxiao | 0.112 0 | - | 1.341 | 0.912 |
马家河Majiehe | 0.298 5 | 0.157 1 | - | 3.247 |
万佛山Wanfoshan | 0.280 0 | 0.215 1 | 0.071 5 | - |
Table 3 Pairwised FST(below diagonal) and Nm (above diagonal) among Berchemiella wilsonii var. pubipetiolata populations
居群 Population | 湍口 Tuankou | 马啸 Maxiao | 马家河 Majiahe | 万佛山 Wanfoshan |
---|---|---|---|---|
湍口Tuankou | - | 1.982 | 0.588 | 0.643 |
马啸Maxiao | 0.112 0 | - | 1.341 | 0.912 |
马家河Majiehe | 0.298 5 | 0.157 1 | - | 3.247 |
万佛山Wanfoshan | 0.280 0 | 0.215 1 | 0.071 5 | - |
变异来源 Source of variance | 自由度 df | 平方和 Sum of squares | 方差分量 Variance component | 方差分量百率(%) Percent of variance component | 显著性检测 p |
---|---|---|---|---|---|
居群间Among populations | 3 | 206.50 | 99.28 | 34.01 | <0.001 |
居群内Within population nested | 85 | 481.43 | 373.76 | 65.99 | <0.001 |
地理宗间Between geographic domes | 1 | 136.79 | 58.02 | 24.88 | 0.176 9 |
地理宗内Among populations within group | 2 | 69.72 | 20.28 | 14.71 | <0.001 |
个体间Among individuals within population | 85 | 481.42 | 342.21 | 60.42 | <0.001 |
Table 4 The results of AMOVA for individuals of Berchemiella wilsonii var. pubipetiolata using 122 AFLP markers
变异来源 Source of variance | 自由度 df | 平方和 Sum of squares | 方差分量 Variance component | 方差分量百率(%) Percent of variance component | 显著性检测 p |
---|---|---|---|---|---|
居群间Among populations | 3 | 206.50 | 99.28 | 34.01 | <0.001 |
居群内Within population nested | 85 | 481.43 | 373.76 | 65.99 | <0.001 |
地理宗间Between geographic domes | 1 | 136.79 | 58.02 | 24.88 | 0.176 9 |
地理宗内Among populations within group | 2 | 69.72 | 20.28 | 14.71 | <0.001 |
个体间Among individuals within population | 85 | 481.42 | 342.21 | 60.42 | <0.001 |
[1] | Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Stuhl K (1987). Current Protocols in Molecular Biology. John Wiley & Sons, New York. |
[2] |
Brown AHD (1978). Isozymes, plant population genetic structure and genetic conservation. Theoretical and Applied Genetics, 52,145-157.
URL PMID |
[3] | Buza L, Young A, Thrall P (2000). Genetic erosion, inbreeding and reduced fitness in fragmented populations of the endangered tetraploid pea Swainsona recta. Biological Conservation, 93,177-186. |
[4] | Chung MG (1999). Allozyme diversity in the endangered shrub Abeliophyllum distichum (Oleaceae): a monotypic Korean genus. International Journal of Plant Science, 160,553-559. |
[5] |
Coart E, Vekemans X, Smulders MJM, Wagner I, van Huylenbroeck J, van Bockstaele E, Roldn-Ruiz I (2003). Genetic variation in the endangered wild apple ( Malus sylvestris (L.) Mill.) in Belgium as revealed by amplified fragment length polymorphism and microsatellite markers. Molecular Ecology, 12,845-857.
DOI URL PMID |
[6] |
Dolan RW, Yahr R, Menges ES, Halfhill MD (1999). Conservation implications of genetic variation in three rare species endemic to Florida rosemary scrub. American Journal of Botany, 86,1556-1562.
URL PMID |
[7] |
Drummond RSM, Keeling DJ, Richardson TE, Gardner RC, Wright SD (2000). Genetic analysisand conservation of 31 surviving individuals of a rare New Zealand tree,Metrosideros bartlettii (Myrtaceae). Molecular Ecology, 9,1149-1157.
URL PMID |
[8] |
Excoffier L, Smouse PE, Quattro JM (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131,479-491.
URL PMID |
[9] | Excoffier L (1995). AMOVA 1.55 (Analysis of Molecular Variance). Genetics and Biometry Laboratory, University of Geneva, Suitzerland. |
[10] | Falk DA, Holsinger KE (1991). Genetics and Conservation of Rare Plants. Oxford University Press, Oxford. |
[11] | Frankham R, Ballou JD, Briscoe DA (2002). Introduction to Conservation Genetics. Cambridge University Press, UK. |
[12] | Ge S (葛颂) (1998). The review and prospect of researches in genetic structure of plant populations. In: Li CS (李承森) ed. Progress in plant scienceⅠ (植物科学进展(第一卷)). Higher Education Press, Beijing,1-15. (in Chinese) |
[13] |
Juan A, Crespo MB, Cowan RS, Lexer C, Fay MF (2004). Patterns of variability and gene flow in Medicago citrina, an endangered endemic of islands in the western Mediterranean, as revealed by amplified fragment length polymorphism (AFLP). Molecular Ecology, 13,2679-2690.
DOI URL PMID |
[14] | Jorgensen SM, Hamrick JL (1997). Biogeography and population genetics of whitebark pine,Pinus albicaulis. Canadian Journal of Forestry Research, 27,1574-1585. |
[15] | Kahmen S, Poschlod P (2000). Population size, plant performance, and genetic variation in the rare plant Arnica montana L. in the Rhøn, Germany. Basic and Applied Ecology, 1,43-51. |
[16] |
Kwon JA, Morden CM (2002). Population genetic structure of two rare tree species ( Colubrina oppositifolia and Alphitonia ponderosa, Rhamnaceae) from Hawaiian dry and mesic forests using random amplified polymorphic DNA markers. Molecular Ecology, 11,991-1001.
URL PMID |
[17] | Lacy RC (1987). Loss of genetic diversity from managed populations: interacting effects of drift, mutation, immigration, selection, and population subdivision. Conservation Biology, 1,143-158. |
[18] | Lande R (1988). Genetics and demography in biological conservation. Science, 241,1455-1460. |
[19] |
Llorens TM, Ayre DJ, Whelan RJ (2004). Evidence for ancient genetic subdivision among recently fragmented populations of the endangered shrub Grevillea caleyi (Proteaceae). Heredity, 92,519-526.
DOI URL PMID |
[20] |
Martinez-Palacios A, Eguiarte LE, Furnier GR (1999). Genetic diversity of the endangered endemic Agave victoriae-regineae (Agavaceae) in the Chihuahuan desert. American Journal of Botany, 86,1093-1098.
URL PMID |
[21] | Miller MP (1997). AMOVA-PREP, a Program for the Preparation of AMOVA Input Files for Use with WINAMOUA. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ. |
[22] |
Mueller UG, Wolfenbarger LL (1999). AFLP genotyping and fingerprinting. Trends in Ecology and Evolution, 14,389-394.
DOI URL PMID |
[23] |
Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89,583-590.
URL PMID |
[24] | Primack RB (1993). Essentials of Conservation Biology. Sinauer Associates, Sunderland, USA. |
[25] | Qian H (钱宏) (1988). A study on the cenus Berchemiella Nakai (Rhamnaceae) endemic to eastern Asia. Bulletin of Botanical Research (植物研究), 8,119-126. (in Chinese with English abstract) |
[26] |
Rogers SO, Bendich AJ (1985). Extraction of DNA from milligram amount of fresh, herbarium and mummified plant tissues. Plant Molecular Biology, 5,69-76.
DOI URL PMID |
[27] | Rohlf FJ (1994). NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System Version 2.0. Exeter Software, Setauket, New York. |
[28] |
Schmidt K, Jensen K (2000). Genetic structure and AFLP variation of remnant populations in the rare plant Pedicularis palustris (Scrophulariaceae) and its relation to population size and reproductive components. American Journal of Botany, 87,678-689.
URL PMID |
[29] | Shea KL, Furnier GR (2002). Genetic variation and population structure in central and isolated populations of balsam fir, Abies balsamea (Pinaceae). American Journal of Botany, 89,783-791. |
[30] |
Sork VL, Nason J, Campbell DR, Fernandez JF (1999). Landscape approaches to historical and contemporary gene flow in plants. Trends in Ecology and Evolution, 14,219-224.
DOI URL PMID |
[31] | Tomimatsu H, Ohara M (2003). Genetic diversity and local population structure of fragmented populations of Trillium camschatcense (Trilliaceae). Biological Conservation, 109,249-258. |
[32] |
Travis SE, Maschinski J, Keim P (1996). An analysis of genetic variation in Astragalus cremnophylax var. cremnophylax, a critically endangered plant, using AFLP markers. Molecular Ecology, 5,735-745.
URL PMID |
[33] | Vasseur L (2001). Allozymic diversity of Allium tricoccum (Ait.) Solander var. burdickii Hanes in isolated populations of Nova Scotia (Canada). Plant Systematics and Evolution, 228,71-79. |
[34] |
Vekemans X, Beauwens T, Lernaire M, Roldan-Ruiz I (2002). Data from amplified fragment length polymorphism (AFLP) makers show indication of size homoplasy and relationship between degree of homoplasy and fragment size. Molecular Ecology, 11,139-151.
URL PMID |
[35] |
Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zebeau M (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23,4407-4414.
URL PMID |
[36] | Vucetich JA, Peterson RO, Waite TA (2000). Is one migrant per generation sufficient for the genetic management of fluctuating populations? Animal Conservation, 3,261-266. |
[37] | Wang Y (王燕), Tang SQ (唐绍清), Li XK (李先琨) (2004). The genetic diversity of the endangered plant Abies yuanbaoshanensis. Biodiversity Science (生物多样性), 12,269-273. (in Chinese with English abstract) |
[38] | Winfield MO, Arnold GM, Cooper F, Leray M, White J, Karp A, Edwards KJ (1998). A study of genetic diversity in Populus nigra subsp. betulifolia in the upper Severn area in the UK using AFLP markers. Molecular Ecology, 7,3-10. |
[39] | Wright S (1969). Evolution and Genetics of Populations Vol.2.The Theory of Gene Frequencies. University of Chicago Press, Chicago. |
[40] |
Young A, Boyle T, Brown T (1996). The population genetic consequences of habitat fragmentation in plants. Trends in Ecology and Evolution, 11,413-418.
URL PMID |
[41] |
Zhivotovsky LA (1999). Estimating population structure in diploids with multilocus DNA markers. Molecular Ecology, 8,907-913.
URL PMID |
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