Chinese Journal of Plant Ecology >
Responses of soil nitrogen in different soil organic matter fractions to long-term nitrogen addition in a semi-arid grassland
Received date: 2021-02-03
Accepted date: 2021-03-30
Online published: 2021-04-28
Supported by
Chinese National Key Development Program for Basic Research(2019YFA0607302);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23080301);National Natural Science Foundation of China(31901138)
Aims Soil nitrogen (N) plays a vital role in regulating the structure and function of ecosystems and is affected by N deposition. Most previous studies focus on the responses of the N content in bulk soil to N deposition, but the responses of the N content in different soil organic matter (SOM) fractions remain unclear. We aimed to investigate how long-term N addition influenced soil N of different SOM fractions in a semi-arid grassland.
Methods A manipulated N addition experiment with 4 levels of N addition (0, 8, 32 and 64 g·m-2·a-1) has been conducted for 13 years in Duolun country, Nei Mongol. SOM was separated to particulate organic matter (POM) and mineral associated organic matter (MAOM) by density fractionation. The plant and soil properties were also measured.
Important findings The results showed that N addition had no significant effect on the carbon (C) content in bulk soil, POM, or MAOM. With increasing levels of N addition, the N content in bulk soil and in POM increased significantly. Furthermore, we found that the increased N content of POM was mainly associated with greater aboveground biomass following N addition. The N content of MAOM is mainly correlated with soil texture, but was not affected by N addition. These results suggest that continuous N addition can increase the soil N in bulk soil, but the increased N is mostly distributed in labile POM pools, which can be vulnerable to land use and climate change.
WU Yun-Tao, YANG Sen, WANG Xin, HUANG Jun-Sheng, WANG Bin, LIU Wei-Xing, LIU Ling-Li . Responses of soil nitrogen in different soil organic matter fractions to long-term nitrogen addition in a semi-arid grassland[J]. Chinese Journal of Plant Ecology, 2021 , 45(7) : 790 -798 . DOI: 10.17521/cjpe.2021.0044
| [1] | Beck T, Joergensen RG, Kandeler E, Makeschin F, Nuss E, Oberholzer HR, Scheu S (1997). An inter-laboratory comparison of ten different ways of measuring soil microbial biomass C. Soil Biology & Biochemistry, 29, 1023-1032. |
| [2] | Brookes PC, Landman A, Pruden G, Jenkinson DS (1985). Chloroform fumigation and the release of soil-nitrogen-A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biology & Biochemistry, 17, 837-842. |
| [3] | Castellano MJ, Mueller KE, Olk DC, Sawyer JE, Six J (2015). Integrating plant litter quality, soil organic matter stabilization, and the carbon saturation concept. Global Change Biology, 21, 3200-3209. |
| [4] | Christensen BT (2001). Physical fractionation of soil and structural and functional complexity in organic matter turnover. European Journal of Soil Science, 52, 345-353. |
| [5] | Diao LW, Li P, Liu WX, Xu S, Qiao CL, Zeng H, Liu LL (2018). Response of plant biomass to nitrogen addition and precipitation increasing under different climate conditions and time scales in grassland. Chinese Journal of Plant Ecology, 42, 818-830. |
| [5] | [ 刁励玮, 李平, 刘卫星, 徐姗, 乔春连, 曾辉, 刘玲莉 (2018). 草地生态系统生物量在不同气候及多时间尺度上对氮添加和增雨处理的响应. 植物生态学报, 42, 818-830.] |
| [6] | Dungait JAJ, Hopkins DW, Gregory AS, Whitmore AP (2012). Soil organic matter turnover is governed by accessibility not recalcitrance. Global Change Biology, 18, 1781-1796. |
| [7] | Forthofer RN, Lee ES (1996). Introduction to biostatistics: a guide to design, analysis, and discovery. Biometrics, 52, 378. DOI: 10.2307/2533181. |
| [8] | Francesca Cotrufo M, Ranalli MG, Haddix ML, Six J, Lugato E (2019). Soil carbon storage informed by particulate and mineral-associated organic matter. Nature Geoscience, 12, 989-994. |
| [9] | Francesca Cotrufo M, Soong JL, Horton AJ, Campbell EE, Haddix ML, Wall DH, Parton WJ (2015). Formation of soil organic matter via biochemical and physical pathways of litter mass loss. Nature Geoscience, 8, 776-779. |
| [10] | Frey SD, Ollinger S, Nadelhoffer K, Bowden R, Brzostek E, Burton A, Caldwell BA, Crow S, Goodale CL, Grandy AS, Finzi A, Kramer MG, Lajtha K, LeMoine J, Martin M, McDowell WH, Minocha R, Sadowsky JJ, Templer PH, Wickings K (2014). Chronic nitrogen additions suppress decomposition and sequester soil carbon in temperate forests. Biogeochemistry, 121, 305-316. |
| [11] | Galloway JN, Townsend AR, Erisman JW, Bekunda M, Cai Z, Freney JR, Martinelli LA, Seitzinger SP, Sutton MA (2008). Transformation of the nitrogen cycle: recent trends, questions, and potential solutions. Science, 320, 889-892. |
| [12] | Hassink J (1997). The capacity of soils to preserve organic C and N by their association with clay and silt particles. Plant and Soil, 191, 77-87. |
| [13] | Kleber M, Eusterhues K, Keiluweit M, Mikutta C, Mikutta R, Nico PS (2015). Mineral-organic associations: formation, properties, and relevance in soil environments. Advances in Agronomy, 130, 1-140. |
| [14] | Kuzyakov Y, Xu XL (2013). Competition between roots and microorganisms for nitrogen: mechanisms and ecological relevance. New Phytologist, 198, 656-669. |
| [15] | Lavallee JM, Soong JL, Cotrufo MF (2020). Conceptualizing soil organic matter into particulate and mineral-associated forms to address global change in the 21st century. Global Change Biology, 26, 261-273. |
| [16] | Liu LL, Greaver TL (2010). A global perspective on belowground carbon dynamics under nitrogen enrichment. Ecology Letters, 13, 819-828. |
| [17] | Liu MQ, Hu F, Chen XY (2007). A review on mechanisms of soil organic carbon stabilization. Acta Ecologica Sinica, 27, 2642-2650. |
| [17] | [ 刘满强, 胡锋, 陈小云 (2007). 土壤有机碳稳定机制研究进展. 生态学报, 27, 2642-2650.] |
| [18] | Liu WX, Qiao CL, Yang S, Bai WM, Liu LL (2018). Microbial carbon use efficiency and priming effect regulate soil carbon storage under nitrogen deposition by slowing soil organic matter decomposition. Geoderma, 332, 37-44. |
| [19] | Lu M, Yang YH, Luo YQ, Fang CM, Zhou XH, Chen JK, Yang X, Li B (2011). Responses of ecosystem nitrogen cycle to nitrogen addition: a meta-analysis. New Phytologist, 189, 1040-1050. |
| [20] | Meyer S, Leifeld J, Bahn M, Fuhrer J (2012). Free and protected soil organic carbon dynamics respond differently to abandonment of mountain grassland. Biogeosciences, 9, 853-865. |
| [21] | Mikutta R, Kaiser K (2011). Organic matter bound to mineral surfaces: resistance to chemical and biological oxidation. Soil Biology & Biochemistry, 43, 1738-1741. |
| [22] | Mikutta R, Turner S, Schippers A, Gentsch N, Meyer-Stüve S, Condron LM, Peltzer DA, Richardson SJ, Eger A, Hempel G, Kaiser K, Klotzbücher T, Guggenberger G (2019). Microbial and abiotic controls on mineral-associated organic matter in soil profiles along an ecosystem gradient. Scientific Reports, 9, 10294. DOI: 10.1038/s41598-019-46501-4. |
| [23] | Näsholm T, Kielland K, Ganeteg U (2009). Uptake of organic nitrogen by plants. New Phytologist, 182, 31-48. |
| [24] | Niu SL, Classen AT, Dukes JS, Kardol P, Liu LL, Luo YQ, Rustad L, Sun J, Tang JW, Templer PH, Thomas RQ, Tian DS, Vicca S, Wang YP, Xia JY, Zaehle S (2016). Global patterns and substrate-based mechanisms of the terrestrial nitrogen cycle. Ecology Letters, 19, 697-709. |
| [25] | Pribyl DW (2010). A critical review of the conventional SOC to SOM conversion factor. Geoderma, 156, 75-83. |
| [26] | Riggs CE, Hobbie SE, Bach EM, Hofmockel KS, Kazanski CE (2015). Nitrogen addition changes grassland soil organic matter decomposition. Biogeochemistry, 125, 203-219. |
| [27] | Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011). Persistence of soil organic matter as an ecosystem property. Nature, 478, 49-56. |
| [28] | Six J, Callewaert P, Lenders S, de Gryze S, Morris SJ, Gregorich EG, Paul EA, Paustian K (2002). Measuring and understanding carbon storage in afforested soils by physical fractionation. Soil Science Society of America Journal, 66, 1981-1987. |
| [29] | Sollins P, Kramer MG, Swanston C, Lajtha K, Filley T, Aufdenkampe AK, Wagai R, Bowden RD (2009). Sequential density fractionation across soils of contrasting mineralogy: evidence for both microbial- and mineral-controlled soil organic matter stabilization. Biogeochemistry, 96, 209-231. |
| [30] | Vance ED, Brookes PC, Jenkinson DS (1987). An extraction method for measuring soil microbial biomass C. Soil Biology & Biochemistry, 19, 703-707. |
| [31] | von Lützow M, Kögel-Knabner I, Ekschmitt K, Flessa H, Guggenberger G, Matzner E, Marschner B (2007). SOM fractionation methods: relevance to functional pools and to stabilization mechanisms. Soil Biology & Biochemistry, 39, 2183-2207. |
| [32] | Wei C, Yu Q, Bai E, Lü X, Li Q, Xia J, Kardol P, Liang W, Wang Z, Han X (2013). Nitrogen deposition weakens plant-microbe interactions in grassland ecosystems. Global Change Biology, 19, 3688-3697. |
| [33] | Wiesmeier M, Munro S, Barthold F, Steffens M, Schad P, Kögel-Knabner I (2015). Carbon storage capacity of semi-arid grassland soils and sequestration potentials in Northern China. Global Change Biology, 21, 3836-3845. |
| [34] | Yang S, Liu WX, Qiao CL, Wang J, Deng MF, Zhang BB, Liu LL (2019). The decline in plant biodiversity slows down soil carbon turnover under increasing nitrogen deposition in a temperate steppe. Functional Ecology, 33, 1362-1372. |
| [35] | Yu GR, Jia YL, He NP, Zhu JX, Chen Z, Wang QF, Piao SL, Liu XJ, He HL, Guo XB, Wen Z, Li P, Ding GA, Goulding K (2019). Stabilization of atmospheric nitrogen deposition in China over the past decade. Nature Geoscience, 12, 424-429. |
| [36] | Zhang TA, Chen HYH, Ruan HH (2018). Global negative effects of nitrogen deposition on soil microbes. The ISME Journal, 12, 1817-1825. |
| [37] | Zhang Y, Zheng LX, Liu XJ, Jickells T, Neil Cape J, Goulding K, Fangmeier A, Zhang FS (2008). Evidence for organic N deposition and its anthropogenic sources in China. Atmospheric Environment, 42, 1035-1041. |
/
| 〈 |
|
〉 |
Copyright © 2026 Chinese Journal of Plant Ecology
Tel: 010-62836134, 62836138, E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn
