植物生态学报 ›› 2014, Vol. 38 ›› Issue (2): 201-208.doi: 10.3724/SP.J.1258.2013.00037

• 研究论文 • 上一篇    下一篇

内蒙古草原典型植物对土壤微生物群落的影响

王纳纳1,2, 陈颖1,3, 应娇妍1*, 高勇生2, 白永飞1   

  1. 1中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093;
    2江西农业大学国土资源与环境学院, 南昌 330045;
    3内蒙古大学生命科学学院, 呼和浩特 010021
  • 收稿日期:2012-10-18 修回日期:2013-03-11 出版日期:2014-02-01 发布日期:2014-02-12
  • 通讯作者: 应娇妍 E-mail:yingjy@ibcas.ac.cn
  • 基金资助:

    国家重点基础研究发展计划项目(;国家自然科学基金面上项目;国家自然科学基金重点项目

Effects of typical plant on soil microbial communities in an Inner Mongolia grassland

WANG Na-Na1,2, CHEN Ying1,3, YING Jiao-Yan1*, GAO Yong-Sheng2, and BAI Yong-Fei1   

  1. 1State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    2College of Land Resources and Environment, Jiangxi Agricultural University, Nanchang 330045, China;

    3College of Life Science, Inner Mongolia University, Hohhot 010021, China
  • Received:2012-10-18 Revised:2013-03-11 Online:2014-02-01 Published:2014-02-12
  • Contact: YING Jiao-Yan E-mail:yingjy@ibcas.ac.cn

摘要:

为了分析内蒙古草原不同植物物种对土壤微生物群落的影响, 采用实时荧光定量PCR (real-time PCR)以及末端限制性片段长度多态性分析(terminal restriction fragment length polymorphism, T-RFLP)等分子生物学技术, 测定了退化-恢复样地上几种典型植物的根际土壤和非根际土壤中细菌和真菌的数量及群落结构。结果表明, 不同植物物种对根际和非根际细菌及根际真菌数量均有显著影响。根际土壤中的细菌和真菌数量普遍高于非根际土壤, 尤其以真菌更为明显。对T-RFLP数据进行多响应置换过程(multi-response permutation procedures, MRPP)分析和主成分分析(principal component analysis, PCA), 结果表明, 大多数物种的根际细菌及真菌的群落结构与非根际有明显差异, 并且所有物种的真菌群落可以按根际和非根际明显分为两大类群。此外, 细菌和真菌群落结构在一定程度上存在按物种聚类的现象, 以细菌较为明显。这些结果揭示了不同植物对土壤微生物群落的影响特征, 对理解内蒙古草原地区退化及恢复过程中植被演替引起的土壤性质和功能的变化有一定的帮助。

Abstract:
Aims Vegetation in Inner Mongolia grassland has changed due to overgrazing and climate change. Our objective was to explore the effects of plant species on soil microbial communities in an Inner Mongolia grassland to understand the ecological consequences of vegetation changes in this region.
Methods We investigated the sizes and community structures of bacteria and fungi in the rhizosphere and non-rhizosphere soils of several typical plant species in a degradation-restoration site in Xilin Gol Grassland using real-time PCR and terminal restriction fragment length polymorphism (T-RFLP) analysis.
Important findings Different plant species have significant effects on the sizes of bacterial communities in both rhizosphere and non-rhizosphere soils and size of fungal communities in rhizosphere soil. The abundances of bacteria and fungi in the rhizosphere soil were generally higher than those in non-rhizosphere soils, particularly for fungi. Multi-response permutation procedures and principal component analysis of T-RFLP data showed significant differences of the bacterial and fungal community structure between rhizosphere and non-rhizosphere soil for most species, and the fungal community structures of all samples can be divided into the rhizosphere and non-rhizosphere soils. In addition, the community structure clustered by plant species is more evident for bacteria. These results revealed important effects of plant species on microbial community composition as well as significant differences of microbial community between rhizosphere and non-rhizosphere soils. Findings are helpful for understanding plants-soil-microbe interactions and mechanisms involved in the Inner Mongolia grassland region.