Chin J Plan Ecolo ›› 2016, Vol. 40 ›› Issue (12): 1238-1244.doi: 10.17521/cjpe.2015.0398

• Research Articles • Previous Articles     Next Articles

Effects of nitrogen and silicon application on leaf nitrogen content and net photosynthetic rate of Elymus nutans in alpine meadow

Xiao-Lin SI, Wen-Yin WANG, Xiao-Gang GAO, Dang-Hui XU*   

  1. State Key Laboratory of Grassland Agro-ecosystems, School of Life Science, Lanzhou University, Lanzhou 730000, China
  • Online:2016-12-30 Published:2016-12-31
  • Contact: Dang-Hui XU

Abstract: AimsElymus nutans is one of the dominant plant species in alpine meadow. Purpose of this research was to study the effects of nitrogen and silicon application on leaf nitrogen content and net photosynthetic rate in this species to provide scientific basis for fertilization practice in alpine meadow.MethodFour levels nitrogen combined with four levels silicon was applied to E. nutans plants in the alpine meadow. Leaf nitrogen content and net photosynthetic rate of E. nutans were measured.Important findings The results showed that there was a significant improvement in leaf nitrogen content and net photosynthetic rate of the E. nutans with nitrogen or silicon application alone; However, there was a significant interaction between nitrogen and silicon treatments on leaf nitrogen content and net photosynthetic rate; Combining with the three different levels nitrogen, low level silicon (Si1) application did not increase leaf nitrogen content and net photosynthetic rate, but middle level silicon (Si2) could significantly increase the leaf nitrogen content; Combining with the low (N1) or middle (N2) level nitrogen, middle level silicon (Si2) application could significantly increase the net photosynthetic rate; Compared with that control without fertilization, the middle level nitrogen combined with the same level silicon treatment had the highest average of leaf nitrogen content and net photosynthetic rate, which increased by 119.99% and 85.70%, respectively. This study indicated application of nitrogen combined with silicon application enhanced leaf nitrogen content and net photosynthetic rate of E. nutans, and 8 g·m-2silicon application had the best result among other treatments.

Key words: Elymus nutans, nitrogen and silicon application, interaction, leaf nitrogen content, net photosynthetic rate

Table 1

The combination of nitrogen and silicon application"

硅肥 H2SiO3 (g·m-2) 氮肥 NH4NO3 (g·m-2)
0 (N0) 30 (N1) 60 (N2) 90 (N3)
0 (Si0) N0Si0 N1Si0 N2Si0 N3Si0
4 (Si1) N0Si1 N1Si1 N2Si1 N3Si1
8 (Si2) N0Si2 N1Si2 N2Si2 N3Si2
12 (Si3) N0Si3 N1Si3 N2Si3 N3Si3

Fig. 1

Effect of leaf nitrogen content of Elymus nutans with nitrogen and silicon added separately (mean ± SD). Different lowercase letters indicate significant differences between treatments (p < 0.05). N0-N3, Si0-Si3 see Table 1."

Fig. 2

Effect of leaf nitrogen content of Elymus nutans with nitrogen combined with silicon application (mean ± SD). Different lowercase letters indicate significant differences between different silicon treatments with the same nitrogen application (p < 0.05). N1-N3, Si0-Si3 see Table 1."

Table 2

Analysis of interaction between different levels of nitrogen combined with silicon for leaf nitrogen content of Elymus nutans"

来源 Source 第三类平方和 Type III sum of squares d.f. 平均值平方 Mean square F 显著性 Sig.
修正的模型 Corrected Model 1 961.255a 15 130.750 155.011 0.000
截距 Intercept 33 723.940 1 33 723.940 39 981.400 0.000
N 1 860.226 3 620.075 735.130 0.000
Si 81.426 3 27.142 32.178 0.000
N × Si 19.603 9 2.178 2.582 0.016
错误 Error 40.488 48 0.843
总计 Total 35 725.682 64
校正后总数 Corrected total 2 001.742 63

Fig. 3

Effect of net photosynthetic rate of Elymus nutans with nitrogen and silicon added separately (mean ± SD). Different lowercase letters indicate significant differences between treatments (p < 0.05). N0-N3, Si0-Si3 see Table 1."

Table 3

Analysis of interaction in different levels of nitrogen combined with silicon for net photosynthetic rate of Elymus nutans"

来源 Source 第三类平方和 Type III sum of squares d.f. 平均值平方 Mean square F 显著性 Sig.
修正的模型 Corrected Model 3 196.442 a 15 213.096 60.984 0.000
截距 Intercept 114 412.392 1 114 412.392 32 742.537 0.000
N 2 787.497 3 929.166 265.909 0.000
Si 306.681 3 102.227 29.225 0.000
N × Si 102.263 9 11.363 3.252 0.004
错误 Error 167.727 48 3.494
总计 Total 117 776.560 64
校正后总数 Corrected total 3 363.168 63

Fig. 4

Effect of net photosynthetic rate of Elymus nutans with nitrogen combined with Silicon treatments (mean ± SD). Different lowercase letters indicate significant differences between different silicon treatments with the same nitrogen addition (p < 0.05). N1-N3, Si0-Si3 see Table 1."

Fig. 5

Relationships between leaf nitrogen content and net photosynthetic rate in different nitrogen application with silicon addition. N0-N3 see Table 1."

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