Effects of nitrogen and silicon application on leaf nitrogen content and net photosynthetic rate of Elymus nutans in alpine meadow

doi:10.17521/cjpe.2015.0398

Abstract

Abstract: AimsElymus nutans is one of the dominant plant species in alpine meadow. Purpose of this research was to study the effects of nitrogen and silicon application on leaf nitrogen content and net photosynthetic rate in this species to provide scientific basis for fertilization practice in alpine meadow.MethodFour levels nitrogen combined with four levels silicon was applied to E. nutans plants in the alpine meadow. Leaf nitrogen content and net photosynthetic rate of E. nutans were measured.Important findings The results showed that there was a significant improvement in leaf nitrogen content and net photosynthetic rate of the E. nutans with nitrogen or silicon application alone; However, there was a significant interaction between nitrogen and silicon treatments on leaf nitrogen content and net photosynthetic rate; Combining with the three different levels nitrogen, low level silicon (Si₁) application did not increase leaf nitrogen content and net photosynthetic rate, but middle level silicon (Si₂) could significantly increase the leaf nitrogen content; Combining with the low (N₁) or middle (N₂) level nitrogen, middle level silicon (Si₂) application could significantly increase the net photosynthetic rate; Compared with that control without fertilization, the middle level nitrogen combined with the same level silicon treatment had the highest average of leaf nitrogen content and net photosynthetic rate, which increased by 119.99% and 85.70%, respectively. This study indicated application of nitrogen combined with silicon application enhanced leaf nitrogen content and net photosynthetic rate of E. nutans, and 8 g·m^-2silicon application had the best result among other treatments.

http://jtp.cnki.net/bilingual/detail/html/ZWSB201612003

Key words: Elymus nutans, nitrogen and silicon application, interaction, leaf nitrogen content, net photosynthetic rate

Xiao-Lin SI, Wen-Yin WANG, Xiao-Gang GAO, Dang-Hui XU. Effects of nitrogen and silicon application on leaf nitrogen content and net photosynthetic rate of Elymus nutans in alpine meadow[J]. Chin J Plant Ecol, 2016, 40(12): 1238-1244.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2015.0398

https://www.plant-ecology.com/EN/Y2016/V40/I12/1238

Figures/Tables 8

Table 1 The combination of nitrogen and silicon application

硅肥 H₂SiO₃ (g·m^-2)	氮肥 NH₄NO₃(g·m^-2)
硅肥 H₂SiO₃ (g·m^-2)	0 (N₀)	30 (N₁)	60 (N₂)	90 (N₃)
0 (Si₀)	N₀Si₀	N₁Si₀	N₂Si₀	N₃Si₀
4 (Si₁)	N₀Si₁	N₁Si₁	N₂Si₁	N₃Si₁
8 (Si₂)	N₀Si₂	N₁Si₂	N₂Si₂	N₃Si₂
12 (Si₃)	N₀Si₃	N₁Si₃	N₂Si₃	N₃Si₃

Fig. 1 Effect of leaf nitrogen content of Elymus nutans with nitrogen and silicon added separately (mean ± SD). Different lowercase letters indicate significant differences between treatments (p < 0.05). N0-N3, Si0-Si3 see Table 1.

Fig. 2 Effect of leaf nitrogen content of Elymus nutans with nitrogen combined with silicon application (mean ± SD). Different lowercase letters indicate significant differences between different silicon treatments with the same nitrogen application (p < 0.05). N1-N3, Si0-Si3 see Table 1.

Table 2 Analysis of interaction between different levels of nitrogen combined with silicon for leaf nitrogen content of Elymus nutans

来源 Source	第三类平方和 Type III sum of squares	d.f.	平均值平方 Mean square	F	显著性 Sig.
修正的模型 Corrected Model	1 961.255^a	15	130.750	155.011	0.000
截距 Intercept	33 723.940	1	33 723.940	39 981.400	0.000
N	1 860.226	3	620.075	735.130	0.000
Si	81.426	3	27.142	32.178	0.000
N × Si	19.603	9	2.178	2.582	0.016
错误 Error	40.488	48	0.843
总计 Total	35 725.682	64
校正后总数 Corrected total	2 001.742	63

Fig. 3 Effect of net photosynthetic rate of Elymus nutans with nitrogen and silicon added separately (mean ± SD). Different lowercase letters indicate significant differences between treatments (p < 0.05). N0-N3, Si0-Si3 see Table 1.

Table 3 Analysis of interaction in different levels of nitrogen combined with silicon for net photosynthetic rate of Elymus nutans

来源 Source	第三类平方和 Type III sum of squares	d.f.	平均值平方 Mean square	F	显著性 Sig.
修正的模型 Corrected Model	3 196.442^a	15	213.096	60.984	0.000
截距 Intercept	114 412.392	1	114 412.392	32 742.537	0.000
N	2 787.497	3	929.166	265.909	0.000
Si	306.681	3	102.227	29.225	0.000
N × Si	102.263	9	11.363	3.252	0.004
错误 Error	167.727	48	3.494
总计 Total	117 776.560	64
校正后总数 Corrected total	3 363.168	63

Fig. 4 Effect of net photosynthetic rate of Elymus nutans with nitrogen combined with Silicon treatments (mean ± SD). Different lowercase letters indicate significant differences between different silicon treatments with the same nitrogen addition (p < 0.05). N1-N3, Si0-Si3 see Table 1.

Fig. 5 Relationships between leaf nitrogen content and net photosynthetic rate in different nitrogen application with silicon addition. N0-N3 see Table 1.

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