植物生态学报 ›› 2006, Vol. 30 ›› Issue (1): 140-146.DOI: 10.17521/cjpe.2006.0020
收稿日期:
2005-06-01
接受日期:
2005-10-17
出版日期:
2006-06-01
发布日期:
2006-01-30
通讯作者:
贾桂霞
作者简介:
*E-mail:guixiajia@bjfu.edu.cn基金资助:
HE Heng-Bin1, JIA Kun-Feng2, JIA Gui-Xia1,*(), DING Qiong3
Received:
2005-06-01
Accepted:
2005-10-17
Online:
2006-06-01
Published:
2006-01-30
Contact:
JIA Gui-Xia
摘要:
沙冬青(Ammopiptanthus mongolicus)是西北荒漠地区珍稀常绿阔叶灌木,综合沙冬青分布区内气候、土壤和水分等因子,选取宁夏中卫县沙坡头、内蒙古磴口县、内蒙古阿拉善左旗、内蒙古乌拉特后旗作为样区,于2004年4月中下旬沙冬青的花期调查和收集根瘤,并结合地理环境对分离得到的根瘤菌进行抗逆性初步分析。调查发现根瘤的生成及其在宿主植物上的着生部位、大小、形状、颜色等因生境条件的不同而有所差异,水分是其主要的影响因子;分离得到根瘤菌17株,对其耐盐性、耐酸碱性、生长温度范围和抗生素抗性进行了研究。结果表明,64.7%的菌株可以在含3%NaCl的YMA培养基上生长;94.1%的菌株可以在pH 5~11的范围内生长;全部菌株在60 ℃处理10 min后仍能生长;不同菌株对不同抗生素表现出不同的抗性,ZW4和WH41对各抗生素表现出较强的抗性。分离自磴口县的根瘤菌普遍表现出较强的抗酸碱和抗高温的能力,这是对其环境的适应。本研究证明沙冬青根瘤菌具有较强的抗逆性,但菌株间存在差异,这可能是对沙冬青分布区多种景观生态类型的适应。同时测定了几种伴生植物中间锦鸡儿(Caragana intermedia)、猫头刺(Oxytropis aciphylla)和柠条(Caragana corshinskii)根瘤菌的抗逆性,与沙冬青根瘤菌表现出一定的相似性,具较强的抗逆性,说明生态环境对豆科植物-根瘤菌共生体有重要的影响。沙冬青的高抗性可能与沙冬青根瘤菌的高抗性有关。沙冬青与其根瘤菌的共生关系有利于其植物群落的发育和多样性的维系。
何恒斌, 贾昆峰, 贾桂霞, 丁琼. 沙冬青根瘤菌的抗逆性. 植物生态学报, 2006, 30(1): 140-146. DOI: 10.17521/cjpe.2006.0020
HE Heng-Bin, JIA Kun-Feng, JIA Gui-Xia, DING Qiong. A PRELIMINARY STUDY ON THE STRESS RESISTANCE OF RHIZOBIA ISOLATED FROM AMMOPIPTANTHUS MONGOLICUS. Chinese Journal of Plant Ecology, 2006, 30(1): 140-146. DOI: 10.17521/cjpe.2006.0020
菌株号Strains number | 宿主名Host | 采集地点Sampling places | 地形及土壤Terrain and soil | |||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 沙冬青 Ammopiptanthus mongolicus | 磴口Dengkou | 平坦风蚀沙地,风沙土加粘土Wind erosion moving sand, windy sand soil and clay | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 磴口Dengkou | 平坦风蚀沙地,风沙土加粘土Wind erosion moving sand, windy sand soil and clay | |||||||||||||||||||||||||||||||||||||||||||||
DK4 | 沙冬青A. mongolicus | 磴口Dengkou | 盐渍化滩地,粗砾质风沙土Saline-alkaline land, windy stone-sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK5 | 沙冬青A. mongolicus | 磴口Dengkou | 洪水冲积沟滩地,粉尘风沙土Land caused by seasonal floods, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK6 | 沙冬青A. mongolicus | 磴口Dengkou | 洪水冲积沟滩地,粉尘风沙土Land caused by seasonal floods, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK8 | 沙冬青A. mongolicus | 磴口Dengkou | 盐渍化滩地,粗砾质风沙土Saline-alkaline land, windy stone-sand soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW4 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW7 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW8 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WH6 | 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
AZ1 | 沙冬青A. mongolicus | 阿拉善左旗Alashanzuoqi | 低山残塬,红土质灰棕漠土Loess hill, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
AZ2 | 沙冬青A. mongolicus | 阿拉善左旗Alashanzuoqi | 残存河床,风沙土Riverbed, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
CAN(参比菌株Reference strain) | 沙冬青A. mongolicus | 磴口Dengkou | 购于中国农业大学Bought from China Agriculture Universituy | |||||||||||||||||||||||||||||||||||||||||||||
WHn | 柠条Caragana korshinskii | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WHj | 锦鸡儿C. intermedia | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZWn | 柠条C. korshinskii | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WHm | 猫头刺Oxytropis aciphylla | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil |
表1 试验菌株采集地点及参比菌株来源
Table 1 Sampling places of the experimental strains and the source of the reference strain
菌株号Strains number | 宿主名Host | 采集地点Sampling places | 地形及土壤Terrain and soil | |||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 沙冬青 Ammopiptanthus mongolicus | 磴口Dengkou | 平坦风蚀沙地,风沙土加粘土Wind erosion moving sand, windy sand soil and clay | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 磴口Dengkou | 平坦风蚀沙地,风沙土加粘土Wind erosion moving sand, windy sand soil and clay | |||||||||||||||||||||||||||||||||||||||||||||
DK4 | 沙冬青A. mongolicus | 磴口Dengkou | 盐渍化滩地,粗砾质风沙土Saline-alkaline land, windy stone-sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK5 | 沙冬青A. mongolicus | 磴口Dengkou | 洪水冲积沟滩地,粉尘风沙土Land caused by seasonal floods, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK6 | 沙冬青A. mongolicus | 磴口Dengkou | 洪水冲积沟滩地,粉尘风沙土Land caused by seasonal floods, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
DK8 | 沙冬青A. mongolicus | 磴口Dengkou | 盐渍化滩地,粗砾质风沙土Saline-alkaline land, windy stone-sand soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW4 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW7 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZW8 | 沙冬青A. mongolicus | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
| 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WH6 | 沙冬青A. mongolicus | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
AZ1 | 沙冬青A. mongolicus | 阿拉善左旗Alashanzuoqi | 低山残塬,红土质灰棕漠土Loess hill, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
AZ2 | 沙冬青A. mongolicus | 阿拉善左旗Alashanzuoqi | 残存河床,风沙土Riverbed, windy sand soil | |||||||||||||||||||||||||||||||||||||||||||||
CAN(参比菌株Reference strain) | 沙冬青A. mongolicus | 磴口Dengkou | 购于中国农业大学Bought from China Agriculture Universituy | |||||||||||||||||||||||||||||||||||||||||||||
WHn | 柠条Caragana korshinskii | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WHj | 锦鸡儿C. intermedia | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
ZWn | 柠条C. korshinskii | 中卫沙坡头Shapotou | 低山坡地,红土质灰棕漠土Hillside, grey brown desert soil | |||||||||||||||||||||||||||||||||||||||||||||
WHm | 猫头刺Oxytropis aciphylla | 乌拉特后旗Wulatehouqi | 平坦石质滩地及沙丘,风沙土和红土质灰棕漠土Flood land and dune, windy sand soil and grey brown desert soil |
菌株 Strains | NaCl (%) | ||||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| + | + | + | - | - | - | - |
| + | + | - | - | - | - | - |
DK4 | + | + | + | + | + | + | - |
DK5 | + | - | - | - | - | - | - |
DK6 | + | - | - | - | - | - | - |
DK8 | + | + | + | + | + | + | + |
ZW4 | + | + | - | - | - | - | - |
ZW7 | + | + | + | + | + | - | - |
ZW8 | + | + | + | + | + | + | + |
| + | - | - | - | - | - | - |
| + | - | - | - | - | - | - |
| - | - | - | - | - | - | - |
| + | - | - | - | - | - | - |
| + | + | + | + | - | - | - |
WH6 | + | + | + | - | - | - | - |
AZ1 | - | - | - | - | - | - | - |
AZ2 | + | + | + | + | + | + | + |
CAN | + | + | + | - | - | - | - |
WHn | + | + | + | + | + | + | - |
WHj | + | + | + | + | + | + | - |
ZWn | - | - | - | - | - | - | - |
WHm | + | + | + | + | + | + | + |
表2 不同浓度的NaCl对根瘤菌生长的影响
Table 2 Effects of NaCl on growth of rhizobium
菌株 Strains | NaCl (%) | ||||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| + | + | + | - | - | - | - |
| + | + | - | - | - | - | - |
DK4 | + | + | + | + | + | + | - |
DK5 | + | - | - | - | - | - | - |
DK6 | + | - | - | - | - | - | - |
DK8 | + | + | + | + | + | + | + |
ZW4 | + | + | - | - | - | - | - |
ZW7 | + | + | + | + | + | - | - |
ZW8 | + | + | + | + | + | + | + |
| + | - | - | - | - | - | - |
| + | - | - | - | - | - | - |
| - | - | - | - | - | - | - |
| + | - | - | - | - | - | - |
| + | + | + | + | - | - | - |
WH6 | + | + | + | - | - | - | - |
AZ1 | - | - | - | - | - | - | - |
AZ2 | + | + | + | + | + | + | + |
CAN | + | + | + | - | - | - | - |
WHn | + | + | + | + | + | + | - |
WHj | + | + | + | + | + | + | - |
ZWn | - | - | - | - | - | - | - |
WHm | + | + | + | + | + | + | + |
菌株 Strains | pH | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 4 | 5 | 6 | 8 | 9 | 10 | 11 | 12 | 13 | |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
DK4 | - | - | - | + | + | + | + | + | + | - |
DK5 | - | - | + | + | + | + | + | + | - | - |
DK6 | - | + | + | + | + | + | + | + | + | - |
DK8 | - | + | + | + | + | + | + | + | + | - |
ZW4 | - | - | + | + | + | + | + | + | + | - |
ZW7 | - | - | + | + | + | + | + | + | - | - |
ZW8 | - | - | + | + | + | + | + | + | + | - |
| - | - | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | - | + | + | + | + | + | + | + | - |
WH6 | - | - | + | + | + | + | + | + | - | - |
AZ1 | - | - | + | + | + | + | + | + | - | - |
AZ2 | - | + | + | + | + | + | + | + | + | - |
CAN | - | - | - | - | + | + | + | + | - | - |
WHn | - | - | - | - | + | + | + | + | + | - |
WHj | - | + | + | + | + | + | + | + | + | - |
ZWn | - | - | + | + | + | + | + | + | - | - |
WHm | - | + | + | + | + | + | + | + | + | - |
表3 不同的pH值对根瘤菌生长的影响
Table 3 Effects of pH on growth of rhizobium
菌株 Strains | pH | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 4 | 5 | 6 | 8 | 9 | 10 | 11 | 12 | 13 | |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
DK4 | - | - | - | + | + | + | + | + | + | - |
DK5 | - | - | + | + | + | + | + | + | - | - |
DK6 | - | + | + | + | + | + | + | + | + | - |
DK8 | - | + | + | + | + | + | + | + | + | - |
ZW4 | - | - | + | + | + | + | + | + | + | - |
ZW7 | - | - | + | + | + | + | + | + | - | - |
ZW8 | - | - | + | + | + | + | + | + | + | - |
| - | - | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | + | + | + | + | + | + | + | - | - |
| - | - | + | + | + | + | + | + | + | - |
WH6 | - | - | + | + | + | + | + | + | - | - |
AZ1 | - | - | + | + | + | + | + | + | - | - |
AZ2 | - | + | + | + | + | + | + | + | + | - |
CAN | - | - | - | - | + | + | + | + | - | - |
WHn | - | - | - | - | + | + | + | + | + | - |
WHj | - | + | + | + | + | + | + | + | + | - |
ZWn | - | - | + | + | + | + | + | + | - | - |
WHm | - | + | + | + | + | + | + | + | + | - |
菌株 Strains | 温度 Temperature (℃) | |||||||
---|---|---|---|---|---|---|---|---|
4 | 12 | 20 | 37 | 45 | 50(10 min) | 60(10 min) | ||
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
DK4 | + | + | + | + | + | + | + | |
DK5 | + | + | + | - | - | + | + | |
DK6 | - | + | + | + | + | + | + | |
DK8 | - | + | + | + | + | + | + | |
ZW4 | + | + | + | + | - | + | + | |
ZW7 | - | + | + | + | - | + | + | |
ZW8 | + | + | + | + | + | + | + | |
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
| - | - | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
WH6 | - | + | + | + | - | + | + | |
AZ1 | + | + | + | + | - | + | + | |
AZ2 | + | + | + | + | - | + | + | |
CAN | + | + | + | + | - | + | + | |
WHn | - | + | + | + | - | + | + | |
WHj | - | + | + | + | - | + | + | |
ZWn | - | - | + | - | - | + | + | |
WHm | - | - | + | + | + | + | + |
表4 温度对根瘤菌生长的影响
Table 4 Effects of temperature on growth of rhizobium
菌株 Strains | 温度 Temperature (℃) | |||||||
---|---|---|---|---|---|---|---|---|
4 | 12 | 20 | 37 | 45 | 50(10 min) | 60(10 min) | ||
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
DK4 | + | + | + | + | + | + | + | |
DK5 | + | + | + | - | - | + | + | |
DK6 | - | + | + | + | + | + | + | |
DK8 | - | + | + | + | + | + | + | |
ZW4 | + | + | + | + | - | + | + | |
ZW7 | - | + | + | + | - | + | + | |
ZW8 | + | + | + | + | + | + | + | |
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
| - | - | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
| - | + | + | + | - | + | + | |
WH6 | - | + | + | + | - | + | + | |
AZ1 | + | + | + | + | - | + | + | |
AZ2 | + | + | + | + | - | + | + | |
CAN | + | + | + | + | - | + | + | |
WHn | - | + | + | + | - | + | + | |
WHj | - | + | + | + | - | + | + | |
ZWn | - | - | + | - | - | + | + | |
WHm | - | - | + | + | + | + | + |
菌株 Strains | Km(μg·ml-1) | Amp(μg·ml-1) | Rif(μg·ml-1) | Cm(μg·ml-1) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | |
| + | + | - | - | + | + | + | + | + | - | - | - | + | + | + | + |
| + | + | + | - | + | + | + | + | + | - | - | - | + | + | + | + |
DK4 | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
DK5 | + | + | - | - | + | + | + | - | - | - | - | - | + | + | - | |
DK6 | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
DK8 | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - |
ZW4 | + | + | + | - | + | + | + | + | + | + | + | - | + | + | + | + |
ZW7 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
ZW8 | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
| - | - | - | - | + | - | - | - | + | - | - | - | + | + | + | + |
| + | - | - | - | + | - | - | - | - | - | - | - | + | + | + | + |
| + | + | - | - | + | + | + | + | - | - | - | - | + | + | + | + |
| + | + | + | - | + | + | + | + | + | + | - | - | + | + | + | + |
| - | - | - | - | + | + | + | + | - | - | - | - | + | - | - | - |
WH6 | + | + | - | - | + | + | + | + | + | - | - | - | + | + | + | - |
AZ1 | + | + | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
AZ2 | + | - | - | - | + | + | + | + | + | - | - | - | + | + | + | + |
CAN | + | - | - | - | + | + | + | + | - | - | - | - | + | + | + | + |
WHn | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
WHj | + | - | - | - | + | + | - | - | - | - | - | - | + | + | + | + |
ZWn | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
WHm | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - |
表5 沙冬青根瘤菌对抗生素的抗性
Table 5 The resistance of Ammopiptanthus mongolicus thtzobia to antibioties
菌株 Strains | Km(μg·ml-1) | Amp(μg·ml-1) | Rif(μg·ml-1) | Cm(μg·ml-1) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | 5 | 50 | 100 | 300 | |
| + | + | - | - | + | + | + | + | + | - | - | - | + | + | + | + |
| + | + | + | - | + | + | + | + | + | - | - | - | + | + | + | + |
DK4 | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
DK5 | + | + | - | - | + | + | + | - | - | - | - | - | + | + | - | |
DK6 | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
DK8 | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - |
ZW4 | + | + | + | - | + | + | + | + | + | + | + | - | + | + | + | + |
ZW7 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
ZW8 | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
| - | - | - | - | + | - | - | - | + | - | - | - | + | + | + | + |
| + | - | - | - | + | - | - | - | - | - | - | - | + | + | + | + |
| + | + | - | - | + | + | + | + | - | - | - | - | + | + | + | + |
| + | + | + | - | + | + | + | + | + | + | - | - | + | + | + | + |
| - | - | - | - | + | + | + | + | - | - | - | - | + | - | - | - |
WH6 | + | + | - | - | + | + | + | + | + | - | - | - | + | + | + | - |
AZ1 | + | + | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
AZ2 | + | - | - | - | + | + | + | + | + | - | - | - | + | + | + | + |
CAN | + | - | - | - | + | + | + | + | - | - | - | - | + | + | + | + |
WHn | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - |
WHj | + | - | - | - | + | + | - | - | - | - | - | - | + | + | + | + |
ZWn | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - |
WHm | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - |
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