沙冬青及其伴生植物深色有隔内生真菌物种多样性

doi:10.17521/cjpe.2016.0270

摘要/Abstract

摘要：

2013年7月从内蒙古磴口, 宁夏银川、沙坡头和甘肃民勤采集沙蒿(Artemisia desertorum)、红花锦鸡儿(Caragana rosea)、白刺(Nitraria tangutorum)、柠条锦鸡儿(Caragana korshinskii)等4种沙冬青(Ammopiptanthus mongolicus)伴生植物的根系样品, 采用形态特征结合rDNA ITS序列分析进行真菌分类鉴定, 研究了深色有隔内生真菌(DSE)群落组成和物种多样性。从4种伴生植物根样中共分离鉴定7类DSE(即Alternaria、Curvularia、Embellisia、Phialocephala、Phoma、Podospora和Pleosporales), 其中Alternaria、Embellisia、Curvularia和Podospora为荒漠生态系统首次报道的DSE。同一样地伴生植物与沙冬青DSE群落组成差异明显, 不同样地沙冬青与伴生植物DSE物种多样性指数差异显著。结果表明, 自然条件下沙冬青DSE定殖率和物种数量, 特别是产孢种类和数量多于其伴生植物, 暗示DSE能够与沙冬青根系形成更好的共生关系, 增强沙冬青对极端荒漠环境的生态适应性。

关键词: 深色有隔内生真菌, 群落组成, 物种多样性, 沙冬青, 伴生植物

Abstract:

Aims Dark septate endophytes (DSE) extensively colonize in plant root tissues in different ecosystems. However, our understanding of the ecological significance of DSE in semiarid and arid lands is limited. The main objective of this study was to compare the community composition and species diversity of DSE in Ammopiptanthus mongolicus and its companion plants in desert habitats. Methods Healthy root samples of Ammopiptanthus mongolicus’s companion plants, Artemisia desertorum, Caragana rosea, Nitraria tangutorum and Caragana korshinskii, were collected separately from four main locations of Ammopiptanthus mongolicus plantation in Dengkou of Nei Mongol, Minqin of Gansu, Yinchuan and Shapotou of Ningxia in July 2013. Important findings A total of 103 strains belonging to seven species (i.e Alternaria, Curvularia, Embellisia, Phialocephala, Phoma, Podospora and Pleosporales) of DSE were isolated from the four types of companion plants by means of morphological identification and molecular identification. Among the seven genera, Alternaria, Embellisia, Curvularia and Podospora are found for the first time in desert ecosystems. Ammopiptanthus mongolicus and its companion plants at the same site had significantly different community composition of DSE. Ammopiptanthus mongolicus and its companion plants at different sites had significantly different DSE diversity index. The colonization and species richness of DSE, especially the species and the quantity of spores were higher in roots of Ammopiptanthus mongolicus than in its companion plants. This study supports the results that DSE can form a better symbiotic relationship with the roots of Ammopiptanthus mongolicus than with its companion plants, and can enhance the ecological adaptability of Ammopiptanthus mongolicus to the extreme desert environment.

Key words: dark septate endophyte, community composition, species diversity, Ammopiptanthus mongolicus, companion plants

孙茜, 薛子可, 解琳琳, 贺学礼, 赵丽莉. 沙冬青及其伴生植物深色有隔内生真菌物种多样性. 植物生态学报, 2017, 41(7): 729-737. DOI: 10.17521/cjpe.2016.0270

Qian SUN, Zi-Ke XUE, Lin-Lin XIE, Xue-Li HE, Li-Li ZHAO. Diversity of dark septate endophyte in the roots of Ammopiptanthus mongolicus and its companion plants. Chinese Journal of Plant Ecology, 2017, 41(7): 729-737. DOI: 10.17521/cjpe.2016.0270

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2016.0270

https://www.plant-ecology.com/CN/Y2017/V41/I7/729

图/表 9

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样地 Site	伴生植物 Companion plant	经纬度 Latitude and longitude	海拔 Elevation (m)	年降水量 Mean annual precipitation (mm)	平均气温 Mean air temperature (℃)	土壤类型 Soil type	植被类型 Vegetation type
内蒙古磴口 Dengkou, Nei Mongol	沙蒿 Artemisia desertorum	40.43° N, 106.52° E	1 043	145	7.6	流动沙丘 Moving dune	旱生灌木, 半灌木 Desert shrub, subshrub
甘肃民勤 Minqin, Gansu	红花锦鸡儿 Caragana rosea	38.58° N, 102.93° E	1 350	113	9.6	荒漠沙质 Sandy desert	旱生灌木, 半灌木 Desert shrub, subshrub
宁夏银川 Yinchuan, Ningxia	白刺 Nitraria tangutorum	38.6° N, 106.57° E	1 169	203	8.5	石质为主 Stony mainly	荒漠草地, 荒漠灌木 Desert grassland, desert shrub
宁夏沙坡头 Shapotou, Ningxia	柠条锦鸡儿 Caragana korshinskii	37.57° N, 104.97° E	1 298	186	9.5	沙土为主 Sand mainly	旱生灌木, 半灌木 Desert shrub, subshrub

样地 Site	伴生植物 Companion plant	经纬度 Latitude and longitude	海拔 Elevation (m)	年降水量 Mean annual precipitation (mm)	平均气温 Mean air temperature (℃)	土壤类型 Soil type	植被类型 Vegetation type
内蒙古磴口 Dengkou, Nei Mongol	沙蒿 Artemisia desertorum	40.43° N, 106.52° E	1 043	145	7.6	流动沙丘 Moving dune	旱生灌木, 半灌木 Desert shrub, subshrub
甘肃民勤 Minqin, Gansu	红花锦鸡儿 Caragana rosea	38.58° N, 102.93° E	1 350	113	9.6	荒漠沙质 Sandy desert	旱生灌木, 半灌木 Desert shrub, subshrub
宁夏银川 Yinchuan, Ningxia	白刺 Nitraria tangutorum	38.6° N, 106.57° E	1 169	203	8.5	石质为主 Stony mainly	荒漠草地, 荒漠灌木 Desert grassland, desert shrub
宁夏沙坡头 Shapotou, Ningxia	柠条锦鸡儿 Caragana korshinskii	37.57° N, 104.97° E	1 298	186	9.5	沙土为主 Sand mainly	旱生灌木, 半灌木 Desert shrub, subshrub

样地 Site	伴生植物 Companion plant	菌株数 Number of strains	分离率 Isolation rate (%)
磴口 Dengkou	沙蒿 Artemisia desertorum	49	12.37
民勤 Minqin	红花锦鸡儿 Caragana rosea	11	3.83
银川 Yinchuan	白刺 Nitraria tangutorum	26	9.11
沙坡头 Shapotou	柠条锦鸡儿 Caragana korshinskii	17	4.59

样地 Site	伴生植物 Companion plant	菌株数 Number of strains	分离率 Isolation rate (%)
磴口 Dengkou	沙蒿 Artemisia desertorum	49	12.37
民勤 Minqin	红花锦鸡儿 Caragana rosea	11	3.83
银川 Yinchuan	白刺 Nitraria tangutorum	26	9.11
沙坡头 Shapotou	柠条锦鸡儿 Caragana korshinskii	17	4.59

样地 Site	分离菌株编号 Number of strains	参考物种(登录号) Reference taxa (accession number)	序列相似性 Similarity of sequence (%)
磴口 Dengkou	DK-1	Podospora sp. (JQ354914.1)	97%
	DK-0-10	Alternaria alternate (KF731832.1)	99%
	DK-4	Alternaria sp. (KC584231.1)	99%
民勤 Minqin	MQ-1	Pleosporales sp. (JN859354.1)	97%
民勤 Minqin	MQ-4	Curvularia sp. (JF742784.1)	99%
银川 Yinchuan	YC-1	Alternaria japonica (FJ477839.1)	97%
	YC-2-1	Embellisia sp. (JN859366.1)	99%
	YC-3	Phialocephala fluminis (AF486124.1)	100%
沙坡头 Shapotou	SPT-1	Phoma medicaginis (FJ224118.1)	99%