植物生态学报 ›› 2015, Vol. 39 ›› Issue (9): 878-889.DOI: 10.17521/cjpe.2015.0084

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沙冬青与伴生植物深色有隔内生真菌定殖规律及其与土壤因子的相关性

孙茜, 贺超, 贺学礼*(), 赵丽莉   

  1. 河北大学生命科学学院, 河北保定 071002
  • 收稿日期:2015-03-04 接受日期:2015-08-04 出版日期:2015-09-03 发布日期:2015-09-23
  • 通讯作者: 贺学礼
  • 作者简介:

    * 共同第一作者 Co-first author

  • 基金资助:
    国家自然科学基金(31170488)

Colonization of dark septate endophytes in roots of Ammopiptanthus mongolicus and its associated plants as influenced by soil properties

SUN Qian, HE Chao, HE Xue-Li*(), ZHAO Li-Li   

  1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China
  • Received:2015-03-04 Accepted:2015-08-04 Online:2015-09-03 Published:2015-09-23
  • Contact: Xue-Li HE
  • About author:

    # Co-first authors

摘要:

为了探明荒漠环境深色有隔内生真菌(dark septate endophytes, DSE)生态分布和定殖状况, 于2013年7月从宁夏银川、沙坡头和甘肃民勤地区采集沙冬青(Ammopiptanthus mongolicus)及其伴生植物根围0-10、10-20、20-30、30-40、40-50 cm共5个土层土壤样品和根样, 研究了DSE空间定殖规律及其与土壤因子的相关关系。结果表明: 沙冬青和伴生植物根系能被DSE侵染, DSE分布和定殖具有明显的空间异质性, 并与土壤因子密切相关。沙冬青DSE微菌核结构紧凑, 呈团块状, 而伴生植物DSE微菌核零散分布。同一样地, 沙冬青DSE菌丝定殖率、定殖强度和总定殖率较伴生植物高, 而沙冬青和伴生植物微菌核定殖率无显著差异。不同样地, 沙冬青和伴生植物微菌核定殖率无显著差异, 沙冬青DSE菌丝定殖率、定殖强度和总定殖率为沙坡头>银川>民勤; 伴生植物DSE菌丝定殖率、定殖强度和总定殖率为银川>民勤>沙坡头。通径分析和主成分分析表明, 土壤有机质、总球囊霉素、磷酸酶和速效钾是西北荒漠环境中DSE定殖的主要影响因子。

关键词: 深色有隔内生真菌, 生态分布, 荒漠环境, 沙冬青, 伴生植物

Abstract: <i>Aims</i>

Dark septate endophytes (DSE) are commonly detected in plant roots in different ecosystems. However, so far our knowledge about the ecological significance of DSE in semiarid and arid lands is limited. The main objective of this study was to compare the colonization status and ecological distribution of DSE in Ammopiptanthus mongolicus and its associated plants in desert habitats.

<i>Methods</i>

In July 2013, soil and root samples of A. mongolicus and its associated plants were collected from three sites, including Yinchuan, Shapotou and Minqin located in northwest China. At each sampling site, samples were collected at five depth intervals, 0-10, 10-20, 20-30, 30-40 and 40-50 cm, respectively.

<i>Important findings</i>

The roots of A. mongolicus and its associated plants were infected by DSE which could be characterized by typical septate hyphae and microsclerotia. Microsclerotia of A. mongolicus were compact lumps, while the associated plants had scattered microsclerotia. In the same site, microsclerotia colonization in A. mongolicus and its associated plants showed no significant difference, but hyphal colonization, colonization intensity and total colonization of A. mongolicus were much higher than that of its associated plants. In different sites, the colonization intensity and total colonization of DSE in A. mongolicus exhibited a pattern as Shapotou > Yinchuan > Minqin, and these indexes in associated plants were Yinchuan > Minqin > Shapotou. Plant species and soil layer had significant effects on the colonization and distribution of DSE as well as soil properties. The path coefficient and principal component analysis indicated that soil organic matter, total extractable glomalin, phosphatase and available K are the main influencing factors, which directly affected the colonization of DSE in desert environment, Northwest China.

Key words: dark septate endophytes, ecological distribution, desert environment, Ammopiptanthus mongolicus, associated plant