植物生态学报 ›› 2007, Vol. 31 ›› Issue (4): 748-752.DOI: 10.17521/cjpe.2007.0095

• 论文 • 上一篇    

过氧化氢参与了黑暗诱导的盐地碱蓬叶片甜菜红素积累

王长泉1,2, 赵吉强1, 陈敏1, 王宝山1,*()   

  1. 1 山东师范大学生命科学学院,济南 250014
    2 山东理工大学生命科学学院,山东淄博 255049
  • 收稿日期:2005-09-12 接受日期:2005-09-12 出版日期:2007-09-12 发布日期:2007-07-30
  • 通讯作者: 王宝山
  • 作者简介:* E-mail: bswang@sdnu.edu.cn
  • 基金资助:
    国家自然科学基金项目(30270793);山东省自然科学基金重点项目(Z2004D03);山东理工大学科研基金资助项目

INVOLVEMENT OF HYDROGEN PEROXIDE IN BETACYANIN ACCUMULATION INDUCED BY DARK IN LEAVES OF SUAEDA SALSA

WANG Chang-Quan1,2, ZHAO Ji-Qiang1, CHEN Min1, WANG Bao-Shan1,*()   

  1. 1College of Life Sciences, Shandong Normal University, Ji'nan 250014, China
    2College of Life Sciences, Shandong University of Technology, Zibo, Shandong 255049, China
  • Received:2005-09-12 Accepted:2005-09-12 Online:2007-09-12 Published:2007-07-30
  • Contact: WANG Bao-Shan

摘要:

该文比较研究了黑暗和光照条件下C3盐生植物盐地碱蓬(Suaeda salsa)叶片甜菜红素积累和H2O2含量及其抗氧化酶活性的关系,实验分析了甜菜红素体外抗氧化性能,以期揭示诱导盐地碱蓬甜菜红素积累的可能机制以及甜菜红素积累的生理生态意义。结果表明:暗期处理和营养液中加入一定浓度的H2O2都明显促进盐地碱蓬叶片H2O2含量、甜菜红素的含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,而且叶片中 H2O2含量与甜菜红含量、SOD和CAT活性具有正相关性;盐地碱蓬甜菜红素体外清除羟自由基的能力明显强于维生素C,而清除超氧阴离子能力低于维生素C。这些结果表明:黑暗作为一种环境胁迫因子诱导盐地碱蓬叶片甜菜红素的积累可能是由自由基介导的,甜菜红素的积累可能与提高植物的抗氧化能力有关。

关键词: 盐地碱蓬, 甜菜红素, 超氧化物歧化酶, 过氧化氢酶, 超氧阴离子, 羟自由基

Abstract:

Aims Betalains are an important class of water-soluble pigments that comprise the red-violet betacyanins and the yellow betaxanthins and accumulate in most families of the Caryophyllales and some higher fungi. However, little is known about the regulation of betalain bio-synthesis by environmental factors in higher plants. Our objective is to explore the mechanism and the biological role of betacyanin accumulation induced by dark in leaves of the C3 halophyte Suaeda salsa.
Methods Seeds of S. salsa were cultured in 24 h dark and 14 h light/10 h dark, and watered with 0, 0.2% and 1.0% H2O2 nutrient solution. Seedlings were harvested three days later and assayed for betacyanin content, H2O2 content, activity of catalase (CAT) and superoxide dismutase (SOD) in leaves.
Important findings Darkness significantly enhanced H2O2 content, betacyanin content, activity of SOD and CAT in S. salsa leaves. Application of different concentrations of H2O2 to the culture solution increased H2O2 content, betacyanin content, activity of SOD and CAT compared to the dark treatment. Betacyanin content and H2O2 content in S. salsa leaves were closely related. Moreover, the scavenging superoxide ($\mathop{{O}}_{2}^{{\mathop{}-{\ •}^{-}}}$) ability of the betacyanins from S. salsa leaves in vitro was lower than that of vitamin C, while the scavenging hydroxyl radical (·OH) ability of the betacyains was much higher than that of vitamin C. These results suggest that betacyanin accumulation in S. salsa leaves grown in dark is possibly induced via reactive oxygen species (ROS) such as H2O2 and that betacyanin is involved in alleviating damage caused by oxidative stress due to its high ROS scavenging ability.

Key words: betacyanins, catalase, hydrogen peroxide, hydroxyl radicals, superoxide dismutase, superoxide, Suaeda salsa