兴安鹿蹄草叶片雪盖初期与雪盖后期保护酶活性的变化规律

doi:10.3773/j.issn.1005-264x.2009.02.013

摘要/Abstract

摘要：

研究了自然生境下生长的兴安鹿蹄草(Pyrola dahurica)叶片在雪盖初期、后期的细胞膜相对透性、膜质过氧化产物丙二醛(MDA)含量、超氧化物歧化酶(SOD)及过氧化物酶(POD)活性的变化, 结果表明: 兴安鹿蹄草顺利越冬取决于两个关键时期, 即雪盖初期和雪盖后期, 雪盖初期包括积雪期和盛雪期, 雪盖后期包括融雪初期和融雪后期。积雪期(11月1日~12月1日)兴安鹿蹄草随着温度的降低叶片中的MDA含量逐渐增高, 细胞膜相对透性增大, SOD和POD活性增强; 进入盛雪期(12月1日以后), 雪盖下温度较高而恒定, 兴安鹿蹄草叶片MDA含量减少, 细胞膜相对透性减低, SOD和POD活性减弱。翌年春天融雪初期(3月1日~3月15日), 雪盖下温度降低, 兴安鹿蹄草叶片中的MDA含量增高, 但低于积雪期; 细胞膜相对透性增大, 且高于积雪期; SOD和POD活性增强, 但低于积雪期; 融雪后期(3月中旬以后)兴安鹿蹄草叶片MDA含量减少, 细胞膜相对透性减低, SOD和POD活性减弱。

关键词: 兴安鹿蹄草, 丙二醛, 超氧化物歧化酶, 过氧化物酶, 细胞膜透性, 雪盖

Abstract:

Aims Pyrola dahurica, an indicator of mixed conifer-broadleaved forest, is an important medicinal species in northeastern China. Our objective is to explore the mechanism of cold hardiness for this evergreen species during winter seasons.

Methods We studied changes in Malondialdehyde (MDA), cell membrane relative conductivity and the activities of peroxidase (POD) and superoxide dismutase (SOD) in the leaves of P. dahurica during two periods (November to December 2005 and March to April 2006) in Maoershan, Harbin, China.

Important findings Safe hibernation of P. dahurica depends on two critical periods, early stage of snow cover (ESSC: November to December in 2005) and late stage of snow cover (LSSC: March to April in 2006). ESSC extends from the beginning of snow cover to the peak of snow cover, and LSSC extends from the early to late stage of snow thaw. MDA content, cell membrane relative conductivity, and SOD and POD activity increased during the period of snow accumulation. Entering the peak period of snow cover, these same factors decreased as the temperature became higher and more constant under the snow. In the early stage of snow thaw in spring (March 1 to 15), temperature decreased and the MDA content increased, cell membrane relative conductivity increased to a higher level than in the period of snow accumulation and SOD and POD activities increased but were lower than in the period of snow accumulation. During the late stage of snow thaw (middle March), MDA content, relative conductivity of cell membrane and SOD and POD activity decreased.

Key words: Pyrola dahurica, MDA, SOD, POD, cell membrane permeability, snow cover

马玉心, 崔大练. 兴安鹿蹄草叶片雪盖初期与雪盖后期保护酶活性的变化规律. 植物生态学报, 2009, 33(2): 355-360. DOI: 10.3773/j.issn.1005-264x.2009.02.013

MA Yu-Xin, CUI Da-Lian. CHANGES OF PROTECTIVE ENZYME ACTIVITY IN PYROLA DAHURICA BEFORE AND AFTER SNOW COVER IN NORTHEASTERN CHINA. Chinese Journal of Plant Ecology, 2009, 33(2): 355-360. DOI: 10.3773/j.issn.1005-264x.2009.02.013

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.02.013

https://www.plant-ecology.com/CN/Y2009/V33/I2/355

图/表 5

图1 2005年初冬至2006年早春积雪的厚度变化及雪盖下温度变化

Fig. 1 2005 early winter to 2006 early spring snowy variation in thickness and variation in temperature under snow cover

图2 雪盖前后兴安露蹄草叶片细胞膜透性的变化

Fig. 2 Changes in cell membrane permeability of Pyrola dahurica before and after snow cover

图3 雪盖前后兴安露蹄草叶片MDA含量的变化

Fig.3 Changes in MDA level of Pyrola dahurica before and after snow cover

图4 雪盖前后兴安鹿蹄草超氧化物歧化酶(SOD)活性变化

Fig. 4 Changes in SOD activity of Pyrola dahurica before and after snow cover

图5 雪盖前后兴安鹿蹄草过氧化物酶(POD)的活性变化

Fig. 5 Changes in POD activity of Pyrola dahurica before and after snow cover

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