植物生态学报 ›› 2009, Vol. 33 ›› Issue (5): 974-983.DOI: 10.3773/j.issn.1005-264x.2009.05.017
收稿日期:
2008-11-27
修回日期:
2009-05-15
出版日期:
2009-11-27
发布日期:
2009-09-30
通讯作者:
温达志
作者简介:
*(dzwen@scbg.ac.cn)基金资助:
ZHANG Ling-Ling, SUN Fang-Fang1,2, WEN Da-Zhi1,*()
Received:
2008-11-27
Revised:
2009-05-15
Online:
2009-11-27
Published:
2009-09-30
Contact:
WEN Da-Zhi
摘要:
田间温室控制条件下研究入侵杂草薇甘菊(Mikania micrantha)和飞机草(Chromolaena odoratum)在不同光照(高、中、低光照)和土壤水分(高、中、低水分含量)交互作用下, 叶片超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性, 脯氨酸(Pro)、谷胱甘肽(GSH)以及膜脂过氧化物丙二醛(MDA)含量的变化, 探讨两种杂草对不同光照和土壤水分交互作用的生理适应性。结果表明: 高光下低水处理的两种杂草MDA含量均显著高于全水处理, 表现出明显的胁迫。此胁迫条件下薇甘菊通过迅速提高SOD和CAT活性, 提高Pro和GSH含量来清除活性氧, 维持代谢平衡, 但不能在干旱条件下维持较长时间; 飞机草的SOD活性、Pro和GSH含量也迅速提高, 在高光缺水的适应性响应中起了重要作用, CAT则未能发挥保护作用。相对于其他光照, 低光显著降低了薇甘菊的SOD、CAT活性和MDA含量, 而对飞机草上述指标的影响较小, 说明低光降低了两种杂草尤其是薇甘菊的抗氧化物代谢水平。
张玲玲, 孙芳芳, 温达志. 薇甘菊和飞机草抗氧化物和膜脂过氧化物对光照和土壤水分的响应. 植物生态学报, 2009, 33(5): 974-983. DOI: 10.3773/j.issn.1005-264x.2009.05.017
ZHANG Ling-Ling, SUN Fang-Fang, WEN Da-Zhi. EFFECTS OF IRRADIANCE AND SOIL MOISTURE ON ANTIOXIDANTS AND MEMBRANE LIPID PEROXIDATION PRODUCTS IN MIKANIA MICRANTHA AND CHROMOLAENA ODORATUM. Chinese Journal of Plant Ecology, 2009, 33(5): 974-983. DOI: 10.3773/j.issn.1005-264x.2009.05.017
时间 Time (d) | 全光照 FI | 中度光照 MI | 低光照 LI | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
全水分 FW | 中度水分 MW | 低水分 LW | 全水分 FW | 中度水分 MW | 低水分 LW | 全水分 FW | 中度水分 MW | 低水分 LW | |||||
光量子 Light quantum (μmol photons·m-2·s-1) | |||||||||||||
薇甘菊 | 0 | 498.0±4.90 | 128.0±2.55 | 17.2±0.97 | |||||||||
Mikania micrantha | 36 | 808.0±16.55 | 276.0±7.48 | 19.0±1.18 | |||||||||
72 | 756.0±2.45 | 256.0±2.45 | 27.0±1.22 | ||||||||||
飞机草 | 0 | 490.0±5.16 | 121.3±1.12 | 17.8±0.92 | |||||||||
Chromolaena odoratum | 36 | 800.0±12.65 | 255.0±4.47 | 20.5±0.45 | |||||||||
72 | 757.5±2.24 | 257.5±2.24 | 27.5±1.29 | ||||||||||
土壤水分含量 Soil water content (%) | |||||||||||||
薇甘菊 | 0 | 23.6±1.27 | 13.7±0.40 | 6.7±0.97 | 22.9±1.04 | 15.9±0.89 | 10.1±1.01 | 23.8±1.42 | 15.2±0.74 | 11.8±1.28 | |||
Mikania micrantha | 36 | 21.2±1.18 | 12.6±0.77 | 4.5±0.67 | 21.8±1.85 | 10.5±0.56 | 6.2±0.80 | 24.9±1.64 | 14.2±1.41 | 10.1±1.41 | |||
72 | 24.4±0.97 | 16.7±0.45 | 4.0±0.59 | 25.6±1.99 | 17.34±0.55 | 6.3±0.88 | 27.4±1.25 | 15.0±1.07 | 7.9±0.67 | ||||
飞机草 | 0 | 21.9±1.52 | 14.0±1.45 | 8.4±0.99 | 23.3±1.15 | 13.5±0.30 | 10.6±0.35 | 26.0±0.83 | 15.7±1.32 | 12.7±1.29 | |||
Chromolaena odoratum | 36 | 24.3±1.37 | 14.1±0.66 | 4.7±0.15 | 24.1±0.70 | 11.2±1.20 | 6.3±0.70 | 24.5±1.11 | 14.2±1.16 | 10.6±0.44 | |||
72 | 27.1±1.15 | 18.9±1.87 | 3.7±0.70 | 28.1±1.38 | 18.4±0.40 | 5.4±1.19 | 28.8±1.04 | 15.0±1.26 | 7.3±0.88 |
表1 不同光照和土壤水分处理下, 试验开始、中期和末期处理样方内光量子密度和土壤水分含量变化
Table 1 Light irradiance and soil water content (measured with time domain reflectometry (TDR)) at the beginning, middle and end of the experiment in the plots under different light and soil water conditions
时间 Time (d) | 全光照 FI | 中度光照 MI | 低光照 LI | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
全水分 FW | 中度水分 MW | 低水分 LW | 全水分 FW | 中度水分 MW | 低水分 LW | 全水分 FW | 中度水分 MW | 低水分 LW | |||||
光量子 Light quantum (μmol photons·m-2·s-1) | |||||||||||||
薇甘菊 | 0 | 498.0±4.90 | 128.0±2.55 | 17.2±0.97 | |||||||||
Mikania micrantha | 36 | 808.0±16.55 | 276.0±7.48 | 19.0±1.18 | |||||||||
72 | 756.0±2.45 | 256.0±2.45 | 27.0±1.22 | ||||||||||
飞机草 | 0 | 490.0±5.16 | 121.3±1.12 | 17.8±0.92 | |||||||||
Chromolaena odoratum | 36 | 800.0±12.65 | 255.0±4.47 | 20.5±0.45 | |||||||||
72 | 757.5±2.24 | 257.5±2.24 | 27.5±1.29 | ||||||||||
土壤水分含量 Soil water content (%) | |||||||||||||
薇甘菊 | 0 | 23.6±1.27 | 13.7±0.40 | 6.7±0.97 | 22.9±1.04 | 15.9±0.89 | 10.1±1.01 | 23.8±1.42 | 15.2±0.74 | 11.8±1.28 | |||
Mikania micrantha | 36 | 21.2±1.18 | 12.6±0.77 | 4.5±0.67 | 21.8±1.85 | 10.5±0.56 | 6.2±0.80 | 24.9±1.64 | 14.2±1.41 | 10.1±1.41 | |||
72 | 24.4±0.97 | 16.7±0.45 | 4.0±0.59 | 25.6±1.99 | 17.34±0.55 | 6.3±0.88 | 27.4±1.25 | 15.0±1.07 | 7.9±0.67 | ||||
飞机草 | 0 | 21.9±1.52 | 14.0±1.45 | 8.4±0.99 | 23.3±1.15 | 13.5±0.30 | 10.6±0.35 | 26.0±0.83 | 15.7±1.32 | 12.7±1.29 | |||
Chromolaena odoratum | 36 | 24.3±1.37 | 14.1±0.66 | 4.7±0.15 | 24.1±0.70 | 11.2±1.20 | 6.3±0.70 | 24.5±1.11 | 14.2±1.16 | 10.6±0.44 | |||
72 | 27.1±1.15 | 18.9±1.87 | 3.7±0.70 | 28.1±1.38 | 18.4±0.40 | 5.4±1.19 | 28.8±1.04 | 15.0±1.26 | 7.3±0.88 |
图1 不同光照和土壤水分处理下薇甘菊(a, b, c)和飞机草(d, e, f)的超氧化物歧化酶(SOD)活性随处理时间的变化(平均值±标准误差, n=3) 低光下, 复水的低水处理(LW-2)薇甘菊全部死亡, 数据缺失 At low light irradiance, the seedlings of M. micrantha under low water treatment (LW-2) died after rewatering, so the data are missing FI、FW、LI、LW、MI、MW: 同表1 See Table 1
Fig. 1 Superoxide dismutase (SOD) activities of Mikania micrantha (a, b, c) and Caromolaena odoratum (d, e, f) under different light and soil water conditions with treatment time (mean ± SE, n=3)
图2 不同光照和土壤水分处理下薇甘菊(a, b, c)和飞机草(d, e, f)的过氧化氢酶(CAT)活性随处理时间的变化(平均值±标准误差, n=3) 图注同图1 Notes see Fig. 1
Fig. 2 Catalase (CAT) activities of Mirania micrantha (a, b, c) and Chromolaena odoratum (d, e, f) under different light and soil water treatments with treatment time (mean ± SE, n=3)
图3 不同光照和土壤水分处理下薇甘菊(a, b, c)和飞机草(d, e, f)的脯氨酸(Pro)含量随处理时间的变化(平均值±标准误差, n=3) 图注同图1 Notes see Fig. 1
Fig. 3 Proline (Pro) contents of Mikania micrantha (a, b, c) and Chromalaena odoratum (d, e, f) under different light and soil water treatments with treatment time (mean ± SE, n=3)
图4 不同光照和土壤水分处理下薇甘菊(a, b, c)和飞机草(d, e, f)的谷胱甘肽(GSH)含量随处理时间的变化 (平均值±标准误差, n=3) 图注同图1 Notes see Fig. 1
Fig. 4 Glutathione (GSH) contents of Mikania micrantha (a, b, c) and Chromolaena odoratum (d, e, f) under different light and soil water treatments with treatment time (mean ± SE, n=3)
图5 不同光照和土壤水分处理下薇甘菊(a, b, c)和飞机草(d, e, f)的丙二醛(MDA)含量随处理时间的变化 (平均值±标准误差, n=3) 图注同图1 Notes see Fig. 1
Fig. 5 Malondialdehyde (MDA) contents of Mikania micrantha (a, b, c) and Chromolaena odoratum (d, e, f) under different light and soil water treatments with treatment time (mean ± SE, n=3)
[1] |
Asada K (1999). The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601-639.
DOI URL PMID |
[2] |
Burke JJ, Hatfield JL (1985). Plant morphological and biochemical responses to field water deficits. III. Effect of foliage temperature and the potential activity of glutathione reductase. Plant Physiology, 79, 415-419.
DOI URL PMID |
[3] |
Byers JE (2002). Physical habitat attribute mediates biotic resistance to non-indigenous species invasion. Oecologia, 130, 146-156.
DOI URL PMID |
[4] |
Cock MJW (1982). Potential biological control agents for Mikania micrantha H.B.K. from the Neotropical region. Tropical Pest Management, 28, 242-254.
DOI URL |
[5] |
Ellman GL (1959). Tissue sulfhydryl groups. Archives Biochemistry and Biophysics, 82, 70-77.
DOI URL |
[6] | Elton C (1958). The Ecology of Invasions by Animals and Plants. Methuen, London. |
[7] |
Fridovich I (1975). Superoxide dismutase. Annual Review of Biochemistry, 44, 147-159.
DOI URL PMID |
[8] | Gamble PE, Burke JJ (1984). Effect of water stress on the chloroplast antioxidant system. I. Alteration in glutathione reductase activity. Plant Physiology, 78, 615-621. |
[9] |
Giannopolitis CN, Ries SK (1977). Superoxide dismutase. II.Purication and quantitative relationship with water-soluble protein in seedlings. Plant Physiology, 59, 315-318.
DOI URL PMID |
[10] | Halliwell B, Chirico S (1993). Lipid peroxidation: its mechanism, measurement, and significance. American Journal of Clinical Neutralization, 57, 715-725. |
[11] | Hao ZB (郝再彬), Cang J (苍晶), Xu Z (徐仲), Zhang D (张达) (2004). Plant Physiology Experiments (植物生理实验). Harbin Institute of Technology Press,Harbin. (in Chinese) |
[12] |
Hayashi F, Ichino T, Osanai M, Wada K (2000). Oscillation and regulation of proline content of P5CS and ProDH gene expressions in the light/dark cycles in Arabodopsis thaliana L. Plant Cell Physiology, 41, 1096-1101.
DOI URL PMID |
[13] |
Janero DR (1990). Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine, 9, 515-540.
DOI URL PMID |
[14] | Li Y (李毅), Men Q (门旗), Luo Y (罗英) (2000). Effects of spatial variability of soil water on irrigation schedule. Agricultural Research in the Arid Areas (干旱地区农业研究), 18, 80-86. (in Chinese with English abstract) |
[15] | Li ZG (李志刚), Zheng QE (郑启恩), Li H (黎桦), Li SL (李素丽) (2006). The characteristics of Eupatorium odoratum community in limestone mountain area of Long’an County in Guangxi. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 14, 196-201. (in Chinese with English abstract) |
[16] | Lin R (林镕), Chen YL (陈艺林), Shi Z (石铸) (1985). Flora of China (Volume 74) (中国植物志第七十四卷). Science Press, Beijing, 56-58. (in Chinese) |
[17] | Lin ZF (林植芳), Li SS (李双顺), Lin GZ (林桂珠), Sun GC (孙谷畴), Guo JY (郭俊彦) (1984). Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves. Acta Botanica Sinica (植物学报), 26, 605-615. (in Chinese with English abstract) |
[18] | Lu P (鲁萍), Sang WG (桑卫国), Ma KP (马克平) (2006). Activity of antioxidant enzymes in the invasive plant under various environment stresses. Acta Ecologica Sinica (生态学报), 26, 3578-3583. (in Chinese with English abstract) |
[19] |
Liu X, Huang B (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science, 40, 503-513.
DOI URL |
[20] |
Mittler R (2002). Oxidative stress, antioxidants and stress tolerance. Trends of Plant Science, 7, 405-410.
DOI URL |
[21] |
Mishra NP, Fatma T, Singhal GS (1995). Development of antioxidative defense system of wheat seedlings in response to high light. Physiologia Plantarum, 95, 77-82.
DOI URL |
[22] |
Noctor G, Foyer CH (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249-279.
DOI URL PMID |
[23] |
Quero JL, Villar R, Mrańön T, Zamora R (2006). Interactions of drought and shade effects on seedlings of four Quercus species: physiological and structural leaf responses. New Phytologist, 170, 819-834.
DOI URL |
[24] | Sakamoto A, Murata N (2002). The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant, Cell and Environment, 25, 163-171. |
[25] |
Smirnoff N (1993). The role of oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125, 27-58.
DOI URL |
[26] | Tang ZC (汤章城) (1984). Accumulation of proline in plants under stressed conditions and its potential significance. Plant Physiology Communications (植物生理学通讯), 1, 15-21. (in Chinese) |
[27] | Vanderwoude C, Scanlan JC, Davis B, Funkhouse S (2005). Plan for National Delimiting Survey for Siam Weed. Natural Resources and Mines Land Protection Services:Queensland Government. |
[28] | Vitousek PM, D’Antonio CM, Loope LL, Westbrooks R (1996). Biological invasion as global environmental change. American Scientist, 84, 468-478. |
[29] | Wang JF (王俊峰), Feng YL (冯玉龙) (2004). The effect of light intensity on biomass allocation, leaf morphology and relative growth rate of two invasive plants. Acta Phytoecologica Sinica (植物生态学报), 28, 781-786. (in Chinese with English abstract) |
[30] | Wang Y (王胤), Yao RL (姚瑞玲) , Liang HK (梁厚宽), Nie JG (聂吉贵) , Yan PD (颜培栋), Yang ZQ (杨章旗) (2007). Effect of osmotic stress on MDA content and SOD activity in Cyclocarya paliurus. Guangxi Forestry Science (广西林业科学), 36, 133-136. (in Chinese with English abstract) |
[31] | Wen DZ (温达志), Ye WH (叶万辉), Feng HL (冯惠玲), Cai CX (蔡楚雄) (2000). Comparison of basic photosynthetic characteristics between exotic invader weed Mikania micrantha and its companion species. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 8, 139-146. (in Chinese with English abstract) |
[32] | Xie MJ (谢明吉), Ke WS (柯文山), Wang WX (王万贤), Xiong ZT (熊治廷), Wu MY (吴明煜), Chen JJ (陈建军) (2005). MDA accumulation and antioxidation capacity of two Elsholtzia splendens populations under copper stress. Chinese Journal of Ecology (生态学杂志), 24, 935-938. (in Chinese with English abstract) |
[33] |
Xie Y, Li Z, William PG, Li D (2000). Invasive species in China―an overview. Biodiversity Conservation, 10, 1317-1341.
DOI URL |
[34] | Xu KY (许凯扬), Ye WH (叶万辉), Duan XW (段学武), Su XG (苏新国), Xu ZF (徐志防) (2004). Physiological adaptation of Alternanthera philoxeroides under water stress condition induced by polyethylene glycol. Journal of Zhejiang University (Agriculture and Life Sciences)(浙江大学学报(农业与生命科学版)), 30, 271-277. (in Chinese with English abstract) |
[35] | Yang QH, Ye WH, Deng X, Cao HL, Zhang Y, Xu KY (2005). Seed germination ecophysiology of Mikania micrantha H.B.K. Botanical Bulletin of Academia Sinica, 46, 293-299. |
[36] | Zan QJ (昝启杰), Wang YJ (王勇军), Wang BS (王伯荪), Liao WB (廖文波), Li MG (李鸣光) (2000). The distribution and harm of the exotic weed Mikania micrantha. Chinese Journal of Ecology (生态学杂志), 19, 58-61. (in Chinese with English abstract) |
[37] | Zeng SX (曾韶西), Wang YR (王以柔), Liu HX (刘鸿先) (1991). Some enzymatic reactions related to chlorophyll degradation in cucumber cotyledons under chilling in the light. Acta Phytophysiologica Sinica (植物生理学报), 17, 177-182. (in Chinese with English abstract) |
[38] |
Zhang LL, Wen DZ (2009). Structural and physiological responses of two invasive weeds Mikania micrantha and Chromolaena odorata to contrasting light and soil water conditions. Journal of Plant Research, 122, 69-79.
DOI URL |
[1] | 胡朝臣, 刘学炎, 类延宝, 谭运洪, 张鹏, 董玉平, 刘丛强. 西双版纳外来入侵植物及其共存种叶片氮、磷化学计量特征[J]. 植物生态学报, 2016, 40(11): 1145-1153. |
[2] | 余香琴, 冯玉龙, 李巧明. 外来入侵植物飞机草的研究进展与展望[J]. 植物生态学报, 2010, 34(5): 591-600. |
[3] | 李钧敏, 董鸣, 钟章成. 入侵植物薇甘菊种群的遗传分化[J]. 植物生态学报, 2007, 31(4): 680-688. |
[4] | 王满莲, 冯玉龙. 紫茎泽兰和飞机草的形态、生物量分配和光合特性对氮营养的响应[J]. 植物生态学报, 2005, 29(5): 697-705. |
[5] | 昝启杰, 王伯荪, 王勇军, 张军丽, 廖文波, 李鸣光. 薇甘菊的危害与田野菟丝子的防除作用[J]. 植物生态学报, 2003, 27(6): 822-829. |
[6] | 张炜银, 王伯荪, 李鸣光, 昝启杰, 王勇军. 台湾相思林和芒草草丛中薇甘菊枝构件的分枝格局及其生物量[J]. 植物生态学报, 2002, 26(3): 346-350. |
[7] | 杨敏生, 裴保华, 程志鹏. 白杨杂种无性系抗寒性生理指标动态分析[J]. 植物生态学报, 1997, 21(4): 367-375. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||
Copyright © 2022 版权所有 《植物生态学报》编辑部
地址: 北京香山南辛村20号, 邮编: 100093
Tel.: 010-62836134, 62836138; Fax: 010-82599431; E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn
备案号: 京ICP备16067583号-19