波浪冠层栽培模式对高油大豆叶片活性氧代谢和膜脂过氧化的影响

doi:10.3773/j.issn.1005-264x.2008.03.017

植物生态学报 ›› 2008, Vol. 32 ›› Issue (3): 673-680.DOI: 10.3773/j.issn.1005-264x.2008.03.017

波浪冠层栽培模式对高油大豆叶片活性氧代谢和膜脂过氧化的影响

苗保河¹^,²^,³(), 李向东¹(), 刘波⁴, 何启平⁵, 朱陶⁵, 刘兴坦⁵, 朱启玉², 乔广法², 樊廷安², 陈成君², 董庆裕¹, 余松烈¹^,^**

1 山东农业大学农学院作物生物学国家重点实验室,农业部小麦栽培生理与遗传改良重点开放实验室, 山东泰安 271018
2 菏泽市农业科学院, 山东菏泽 274000
3 中国科学院植物研究所植被与环境变化国家重点实验室,北京 100093
4 青岛农业大学,青岛 266109
5 菏泽学院, 山东菏泽 274000

收稿日期:2006-11-20 接受日期:2007-08-21 出版日期:2008-11-20 发布日期:2008-05-30
通讯作者: 余松烈
作者简介:*E-mail:miaobaohe@sdau.edu.cn
第一联系人：
与第一作者同等贡献E-mail: lixdong@sdau.edu.cn
基金资助:
山东省院士科研活动经费、国家“863”计划(国科农科函[2005]50号);国家科技攻关计划(2001BA507A);山东省自然科学基金(9908)

EFFECT OF WAVING-CANOPY CULTURAL TYPE ON ACTIVE OXYGEN AND MEMBRANE LIPID METABOLISM PEROXIDATION OF HIGH OIL SOYBEAN LEAVES

MIAO Bao-He¹^,²^,³(), LI Xiang-Dong¹(), LIU Bo⁴, HE Qi-Ping⁵, ZHU Tao⁵, LIU Xing-Tan⁵, ZHU Qi-Yu², QIAO Guang-Fa², FAN Ting-An², CHEN Cheng-Jun², DONG Qing-Yu¹, YU Song-Lie¹^,^**

¹College of Agronomy, Shandong Agricultural University, State Key Laboratory of Crop Biology, Key Laboratory of Wheat Physiology and Genetics Improvement, Ministry of Agriculture, Taian, Shandong 271018, China
²Heze Academy of Agricultural Science, Heze, Shandong 274000, China
³State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
⁴Qingdao Agricultural University, Qingdao 266109, China
⁵Heze College, Heze, Shandong 274000, China

Received:2006-11-20 Accepted:2007-08-21 Online:2008-11-20 Published:2008-05-30
Contact: YU Song-Lie

摘要/Abstract

摘要：

为探明波浪冠层栽培模式增产增效的机理,为其推广应用提供理论依据,2005~2006年以高油大豆(Glycine max)品种‘鲁豆9号’为试验材料,采用大田试验和室内生理生化测定相结合的方法,研究了波浪冠层栽培模式对高油大豆叶片活性氧代谢和膜脂过氧化的影响。结果表明,波浪冠层群体与对照CK相比,可溶性蛋白质含量平均增加1.56 mg·g^-1,增加0.034%,其中结荚期最多,为9.43 mg·g^-1,增加1.35%,差异达到极显著水平;可溶性糖含量平均增加了3.82 mg·g^-1,增加7.18%,其中开花期最多,为12.51 mg·g^-1,高达48.41%,差异达到极显著水平。可溶性蛋白质和可溶性糖含量的变化更利于C、N代谢和源、库、流的协调,其中,C代谢改善和提高的水平高于N代谢。保护性酶过氧化物酶(Peroxidase, POD)活性平均增高20.13%,在结荚期最多,高达46.53%,差异达到极显著水平,POD是关键保护性酶,其保护作用强于超氧化物歧化酶(Superoxide dismutase, SOD)和过氧化氢酶(Catalase, CAT);SOD活性平均增高2.28%;CAT活性平均增高0.48%;膜脂过氧化产物丙二醛(Malondialdehype, MDA)含量降低。保护性酶POD、SOD和CAT活性升高,MDA含量降低,作物清除活性氧的能力增强,延缓衰老。波浪冠层群体与对照保护性酶变化规律一致,即SOD活性高峰出现时期最早,CAT次之,POD最晚,这表明在大豆不同的生育期,不同保护酶分别发挥着不同程度的保护作用。因此,该栽培模式对大豆活性氧代谢和膜脂过氧化的影响明显,有利于作物高产优质高效目标的实现。

关键词: 栽培模式, 波浪冠层, 高油大豆, 保护酶活性, 膜脂过氧化

Abstract:

Aims The objective of our study was better understanding of increased yield and economic benefits of high oil soybean with waving-canopy cultural type.

Methods We used the high-oil soybean (Glycine max cv. Ludou 9) and studied the effects of waving-canopy cultural type on active oxygen and membrane lipid metabolism peroxidation in its leaves in field experiments and laboratory analyses during 2005-2006.

Important findings The average content of soluble protein and soluble sugar was 1.56 and 3.82 mg·g^-1 greater in the waving-canopy groups compared to CK (0.034% and 7.18%, respectively). Their contents increased 1.35% and 48.41% at podding and flowering growth stages, respectively, with all differences being statistically significant. Changes in the content of soluble protein and soluble sugar were much more favorable to C, N metabolism, and coordination of the source, storage and flow and metabolism of C was more meliorated and greater than that of N. Average activities of peroxidase (POD) increased by 20.13%, most increased by 46.53% at podding growth stages, and the difference was statistically significant. It was the key protective enzyme that had more effects than superoxide dismutase (SOD) and catalase (CAT). The average activities of SOD and CAT increased by 2.28% and 0.48%, respectively, while malondialdehype (MDA) content declined, and the ability for improvement against active oxygen increased and senility occurred slowly. POD, SOD and CAT all had the same trends, the activity of SOD appeared earlier than that of CAT and POD, and POD appeared lastly, i.e., these three enzymes had different effects at different growth stages. Therefore, this cultural type significantly affected the active oxygen and membrane lipid metabolism peroxidation of soybean, and it was much more favorable for the high oil soybean to gain high yield, fine quality and economic benefit.

Key words: cultural type, waving-canopy, high oil soybean, activity of protective enzyme, lipid peroxidation

苗保河, 李向东, 刘波, 何启平, 朱陶, 刘兴坦, 朱启玉, 乔广法, 樊廷安, 陈成君, 董庆裕, 余松烈. 波浪冠层栽培模式对高油大豆叶片活性氧代谢和膜脂过氧化的影响. 植物生态学报, 2008, 32(3): 673-680. DOI: 10.3773/j.issn.1005-264x.2008.03.017

MIAO Bao-He, LI Xiang-Dong, LIU Bo, HE Qi-Ping, ZHU Tao, LIU Xing-Tan, ZHU Qi-Yu, QIAO Guang-Fa, FAN Ting-An, CHEN Cheng-Jun, DONG Qing-Yu, YU Song-Lie. EFFECT OF WAVING-CANOPY CULTURAL TYPE ON ACTIVE OXYGEN AND MEMBRANE LIPID METABOLISM PEROXIDATION OF HIGH OIL SOYBEAN LEAVES. Chinese Journal of Plant Ecology, 2008, 32(3): 673-680. DOI: 10.3773/j.issn.1005-264x.2008.03.017

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.03.017

https://www.plant-ecology.com/CN/Y2008/V32/I3/673

图/表 6

图1 波浪冠层群体在不同生育时期可溶性蛋白质的含量大写字母为0.01显著水平,小写字母为0.05显著水平

Fig.1 Soluble protein content of waving-canopy groups at different growth stages Capital letters indicate significant differences at the 0.01 probability level, and small letters indicate significant differences at the 0.05 probability level

图2 波浪冠层群体在不同生育时期可溶性糖的含量图注见图1

Fig.2 Soluble sugar content of waving-canopy groups at different growth stages Notes see Fig. 1

图3 波浪冠层群体在不同生育时期过氧化氢酶(CAT)活性的变化图注见图1

Fig.3 Changes of catalase (CAT) activity of waving-canopy groups at different growth stages Notes see Fig. 1

图4 波浪冠层群体在不同生育时期过氧化物酶(POD)活性的变化图注见图1

Fig.4 Changes of perosidase (POD) activity of waving-canopy groups at different growth stages Notes see Fig. 1

图5 波浪冠层群体在不同生育时期超氧化物歧化酶(SOD)活性的变化图注见图1

Fig.5 Changes of superoxide dismutase (SOD) activity of waving-canopy groups at different growth stages Notes see Fig. 1

图6 波浪冠层群体在不同生育时期丙二醛(MDA)的含量图注见图1

Fig.6 Malondialdehype (MDA) content of waving-canopy groups at different growth stages Notes see Fig. 1

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波浪冠层栽培模式对高油大豆叶片活性氧代谢和膜脂过氧化的影响

EFFECT OF WAVING-CANOPY CULTURAL TYPE ON ACTIVE OXYGEN AND MEMBRANE LIPID METABOLISM PEROXIDATION OF HIGH OIL SOYBEAN LEAVES

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