光质对豇豆幼苗环旋运动的影响

doi:10.17521/cjpe.2018.0038

摘要/Abstract

摘要：

为研究光质对豇豆(Vigna unguiculata)幼苗环旋运动的影响, 分别在暗箱的顶部和侧面固定一台红外检测仪, 在垂直生长方向分别设置白、红、蓝、红-蓝光源, 对豇豆幼苗生长点运动进行48 h的等间隔(5 min)拍照, 读取图片中豇豆幼苗生长点的轨迹坐标进行分析。结果表明: 黑暗下, 豇豆幼苗生长点的运动分为逆时针螺旋上升(约占1/3)和不规则的摆动上升(约占2/3)两类。螺旋上升持续时间为18.3 h, 后转为不规则的摆动上升, 环旋一周的时间为(82.7 ± 4.2) min, 最大摆幅为2.0 cm, 最小摆幅为0.5 cm。白光下为趋向光源摆动上升, 摆动幅度由大到小, 再变大, 最大摆幅为0.6 cm, 最小摆幅为0.1 cm。红光下为近直线趋向光源和垂直光源方向摆动两个阶段, 近直线趋向光源持续(21.9 ± 1.6) h, 垂直光源方向摆动持续(8.8 ± 0.5) h, 最大摆幅为3.5 cm, 最小摆幅为2.0 cm。蓝光下为“Z”形向光源上升运动, (110.0 ± 5.8) min和(223.5 ± 4.9) min两种周期交替进行。红-蓝光下趋向蓝光运动持续(12.0 ± 3.8) h后, 转慢速趋红光运动。不同光质下48 h内豇豆幼苗增长高度由高到低为: 红光>黑暗>蓝光>白光>红-蓝光。豇豆幼苗在没有光照下也产生环旋运动, 环旋运动不需要光的诱导, 但光质改变了幼苗运动的方向和速度。不同光质下豇豆幼苗有不同的运动形式, 但总体上都表现为趋光性。

关键词: 环旋运动, 光质, 三维坐标, 豇豆

Abstract:

Aims The aim of this study is to investigate the effect of different light qualities on circumnutation of cowpea (Vigna unguiculata) seedlings.

Methods The behaviors of cowpea seedlings were observed in a black box, which fixed with two infrared cameras on the top and a side, respectively. The motion was recorded as photos taken at an interval of 5 min, in a 3-dimensional space coordinate under different light qualities (such as white, red, blue, and red-blue with opposite direction) for 48 h. The coordinate values of cowpea seedling growth point (CPGP) were read on a computer screen.

Important findings The results showed that in the dark condition, the CPGP movements had two types, one was a spiral rising anticlockwise circulation, and the other was swinging up irregularly. The spiral rising anticlockwise circulation lasted 18.3 h, then turned to swing up irregularly. It takes (82.7 ± 4.2) min spiraling a circle on average, with the maximum range of swing 2.0 cm and the minimum 0.5 cm. Under white light condition, the CPGP movements tended toward light source with a swing up irregularly. The range of swing changed from decrease to increase, with the maximum range of swing 0.6 cm and the minimum 0.1 cm. Under red light condition, the CPGP movements showed two stages, in which one was a tending toward light source directly for (21.9 ± 1.6) h, the other was swing up perpendicular to light direction for (8.8 ± 0.5) h. The maximum range of swing was 3.5 cm, and the minimum was 2.0 cm. Under blue light condition, the CPGP movements showed a swing up with “Z” trajectory tending toward light source, alternating 2 durations with (110.0 ± 5.8) min and (223.5 ± 4.9) min. under red-blue light with opposite direction conditions, the CPGP movements tended toward blue light source for (12.0 ± 3.8) h, then toward red light source slowly. The order of CPGP within 48 h decreased under different light quality with red, dark, blue, white and red-blue. The seedlings of cowpea could move with circumnutation in dark conditions, suggesting that circumnutation does not need light induction. However, the light quality could change the movement direction and speed. CPGP movements showed different patterns under different light qualities, but the movements tended toward light source in general.

Key words: circumnutation, light quality, 3-dimensional space coordinate, Vigna unguiculata

李奇, 胡飞. 光质对豇豆幼苗环旋运动的影响. 植物生态学报, 2018, 42(12): 1192-1199. DOI: 10.17521/cjpe.2018.0038

LI Qi, HU Fei. Effects of light quality on circumnutation of Vigna unguiculata seedlings. Chinese Journal of Plant Ecology, 2018, 42(12): 1192-1199. DOI: 10.17521/cjpe.2018.0038

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2018.0038

https://www.plant-ecology.com/CN/Y2018/V42/I12/1192

图/表 6

图1 豇豆幼苗环旋运动的空间坐标示意图。A, 侧视图。B, 俯视图。

Fig. 1 Space coordinates diagram of circumnutation of Vigna unguiculata seedlings. A, Side view. B, Top view.

图2 黑暗情况下豇豆幼苗48 h运动轨迹。A, 三维空间运动轨迹。B, 椭圆形运动。C, 不规则运动。?, 坐标原点位置。实线箭头表示运动方向。

Fig. 2 The trajectory of cowpea seedling in the darkness for 48 h. A, Three-dimensional trajectory. B, Elliptical type. C, Irregular type. ?, origin position of coordinates. Solid arrows indicate the direction of the movement.

图3 白光下豇豆幼苗48 h运动轨迹。A, 三维空间运动轨迹。B, 摆动运动。?, 坐标原点位置。实线箭头表示运动方向。光源中心通过坐标原点, 虚线箭头表示光源方向。

Fig. 3 The trajectory of cowpea seedling in the white light for 48 h. A, Three-dimensional trajectory. B, Perpendicular type. ?, origin position of coordinates. Solid arrows indicate the direction of the movement. Light source center went through the origin of coordinates and short dash arrows indicate the light source direction.

图4 红光下豇豆幼苗48 h运动轨迹。A, 三维空间运动轨迹。B, 近直线运动。C, 垂直光源方向摆动型运动。?, 坐标原点位置。光源中心通过坐标原点, 虚线箭头表示光源方向。实线箭头表示运动方向。

Fig. 4 The trajectory of cowpea seedling in the red light for 48 h. A, Three-dimensional trajectory. B, Near linear type. C, Swing up perpendicular to light direction type. ?, origin position of coordinates. Light source center went through the origin of coordinates and short dash arrows indicate the light source direction. Solid arrows indicate the direction of the movement.

图5 蓝光下豇豆幼苗48 h运动轨迹。A, 三维空间运动轨迹。B, “Z”形运动。?, 表示坐标原点位置。光源中心通过坐标原点, 虚线箭头表示光源方向。实线箭头表示运动方向。

Fig. 5 The trajectory of cowpea seedling in the blue light for 48 h. A, Three-dimensional trajectory. B, “Z” shape. ?, origin position of coordinates. Light source center went through the origin of coordinates and short dash arrows indicate the light source direction. Solid arrows indicate the direction of the movement.

图6 红-蓝光下豇豆幼苗48 h运动轨迹。A, 三维空间运动轨迹。B, 趋向蓝光运动。C, 趋向红光运动。BL, 蓝光; RL, 红光。?, 坐标原点位置。光源中心通过坐标原点, 虚线箭头表示光源方向。实线箭头表示运动方向。

Fig. 6 The trajectory of cowpea seedling in the red and blue light for 48 h. A, Three-dimensional trajectory. B, Tending to blue light type. C, Tending to red light type. BL, blue light; RL, red light. ?, origin position of coordinates. Light source center went through the origin of coordinates and short dash arrows indicate the light source direction. Solid arrows indicate the direction of the movement.

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