Chin J Plant Ecol ›› 2012, Vol. 36 ›› Issue (8): 880-890.DOI: 10.3724/SP.J.1258.2012.00880
Special Issue: 生物多样性
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ZOU Yuan-Yuan1, LIU Lin1, LIU Yang1, ZHAO Liang1, DENG Qi-Yun2,*(), WU Jun2, ZHUANG Wen2, SONG Wei1,*(
)
Received:
2011-12-09
Accepted:
2012-04-25
Online:
2012-12-09
Published:
2012-08-21
Contact:
DENG Qi-Yun,SONG Wei
ZOU Yuan-Yuan, LIU Lin, LIU Yang, ZHAO Liang, DENG Qi-Yun, WU Jun, ZHUANG Wen, SONG Wei. Diversity of indigenous bacterial communities in Oryza sativa seeds of different varieties[J]. Chin J Plant Ecol, 2012, 36(8): 880-890.
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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2012.00880
杂交子代 Hybrid lines | 母本 Female parental lines | 父本 Male parental lines |
---|---|---|
‘丰优611’ ‘Fengyou 611’ | ‘丰源A’ ‘Fengyuan A’ | ‘远恢611’ ‘Yuanhui 611’ |
‘金优611’ ‘Jinyou 611’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ |
‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘金23A’ ‘Jin 23A’ | ‘09H013’ ‘09H013’ |
Table 1 Genetic relationship among seven rice varieties of three hybrid combinations
杂交子代 Hybrid lines | 母本 Female parental lines | 父本 Male parental lines |
---|---|---|
‘丰优611’ ‘Fengyou 611’ | ‘丰源A’ ‘Fengyuan A’ | ‘远恢611’ ‘Yuanhui 611’ |
‘金优611’ ‘Jinyou 611’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ |
‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘金23A’ ‘Jin 23A’ | ‘09H013’ ‘09H013’ |
‘丰优611’ ‘Fengyou 611’ | ‘金优611’ ‘Jinyou 611’ | ‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘丰源A’ ‘Fengyuan A’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ | ‘09H013’ | |
---|---|---|---|---|---|---|---|
克隆数 No. of clones | 201 | 268 | 253 | 241 | 278 | 240 | 273 |
OTU数 No. of OTUs | 30 | 38 | 39 | 42 | 43 | 41 | 38 |
C值 C value | 93.03% | 93.66% | 93.28% | 95.02% | 93.88% | 92.08% | 92.31% |
Table 2 Estimation of seeds’ indigenous bacterial 16S rDNA libraries of seven different rice varieties
‘丰优611’ ‘Fengyou 611’ | ‘金优611’ ‘Jinyou 611’ | ‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘丰源A’ ‘Fengyuan A’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ | ‘09H013’ | |
---|---|---|---|---|---|---|---|
克隆数 No. of clones | 201 | 268 | 253 | 241 | 278 | 240 | 273 |
OTU数 No. of OTUs | 30 | 38 | 39 | 42 | 43 | 41 | 38 |
C值 C value | 93.03% | 93.66% | 93.28% | 95.02% | 93.88% | 92.08% | 92.31% |
类群 Group | 菌属 Genus | ‘丰优611’ ‘Fengyou 611’ | ‘金优611’ ‘Jinyou 611’ | ‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘丰源A’ ‘FengyuanA’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ | ‘09H013’ |
---|---|---|---|---|---|---|---|---|
α变形杆菌 α-Proteobacteria | 17.41 | 3.36 | 28.06 | 16.18 | 1.44 | 35.42 | 27.84 | |
甲基杆菌 Methylobacterium | 6.47 | 0.75 | 9.49 | 0.41 | - | 2.92 | 0.73 | |
鞘氨醇单胞菌 Sphingomonas | 6.47 | 0.37 | 4.35 | 0.83 | 1.44 | 5.83 | 2.93 | |
土壤杆菌 Agrobacterium | 4.48 | 1.49 | 13.44 | 10.37 | - | 12.50 | 9.89 | |
橙单胞菌 Aurantimonas | - | - | - | - | - | - | 14.29 | |
沃尔巴克氏体 Wolbachia | - | - | - | - | - | 13.33 | - | |
其他 Others | - | 0.75 | 0.79 | 4.56 | - | 0.83 | - | |
β变形杆菌 β-Proteobacteria | 2.49 | - | 2.77 | 22.41 | 0.72 | 0.83 | 7.69 | |
嗜甲基菌 Methylophilus | - | - | - | 8.30 | - | - | - | |
伯克霍尔德氏菌 Burkholderia | - | - | - | 5.81 | - | - | - | |
其他 Others | 2.49 | - | 2.77 | 8.30 | 0.72 | 0.83 | 7.69 | |
γ变形杆菌 γ-Proteobacteria | 78.61 | 35.82 | 40.71 | 34.44 | 61.51 | 45.83 | 56.78 | |
泛菌 Pantoea | 64.18 | 5.60 | 31.62 | 22.82 | 7.55 | 40.00 | 54.95 | |
假单胞菌 Pseudomonas | 13.43 | 27.99 | 5.93 | 2.07 | 13.31 | 3.75 | - | |
肠杆菌 Enterobacter | - | - | - | - | 17.63 | - | - | |
欧文氏菌 Erwinia | - | 1.12 | - | - | 9.35 | - | - | |
不动杆菌 Acinetobacter | - | 0.37 | - | - | 7.20 | - | - | |
其他 Others | 1.00 | 0.74 | 3.16 | 9.54 | 6.47 | 2.08 | 1.83 | |
放线菌 Actinobacteria | 1.00 | 1.49 | 24.90 | 15.77 | 5.76 | 8.75 | 4.03 | |
微杆菌 Microbacterium | - | 1.12 | 19.37 | 2.07 | - | 7.50 | 4.03 | |
短小杆菌 Curtobacterium | 1.00 | 0.37 | 5.53 | 10.79 | 5.76 | 1.25 | - | |
其他 Others | - | - | - | 2.90 | - | - | - | |
厚壁菌 Firmicutes | - | 57.84 | 0.40 | 3.32 | 29.50 | - | 0.37 | |
芽孢杆菌 Bacillus | - | 57.84 | - | - | 28.78 | - | - | |
其他 Others | - | - | 0.40 | 3.32 | 0.72 | - | 0.37 | |
拟菌 Bacteroidetes | 0.50 | - | 3.16 | 4.98 | - | 9.17 | 3.30 | |
鞘胺醇杆菌 Sphingobacterium | - | - | - | - | - | 7.08 | 1.47 | |
其他 Others | 0.50 | - | 3.16 | 4.98 | - | 2.08 | 1.83 | |
其他 Others | - | 1.49 | - | 2.90 | 1.08 | - | - |
Table 3 Abundance of main genera detected in the seeds’ indigenous bacterial 16S rDNA clone libraries of three hybrid rice varieties and their parents (%)
类群 Group | 菌属 Genus | ‘丰优611’ ‘Fengyou 611’ | ‘金优611’ ‘Jinyou 611’ | ‘金23A/09H013’ ‘Jin 23A/09H013’ | ‘丰源A’ ‘FengyuanA’ | ‘金23A’ ‘Jin 23A’ | ‘远恢611’ ‘Yuanhui 611’ | ‘09H013’ |
---|---|---|---|---|---|---|---|---|
α变形杆菌 α-Proteobacteria | 17.41 | 3.36 | 28.06 | 16.18 | 1.44 | 35.42 | 27.84 | |
甲基杆菌 Methylobacterium | 6.47 | 0.75 | 9.49 | 0.41 | - | 2.92 | 0.73 | |
鞘氨醇单胞菌 Sphingomonas | 6.47 | 0.37 | 4.35 | 0.83 | 1.44 | 5.83 | 2.93 | |
土壤杆菌 Agrobacterium | 4.48 | 1.49 | 13.44 | 10.37 | - | 12.50 | 9.89 | |
橙单胞菌 Aurantimonas | - | - | - | - | - | - | 14.29 | |
沃尔巴克氏体 Wolbachia | - | - | - | - | - | 13.33 | - | |
其他 Others | - | 0.75 | 0.79 | 4.56 | - | 0.83 | - | |
β变形杆菌 β-Proteobacteria | 2.49 | - | 2.77 | 22.41 | 0.72 | 0.83 | 7.69 | |
嗜甲基菌 Methylophilus | - | - | - | 8.30 | - | - | - | |
伯克霍尔德氏菌 Burkholderia | - | - | - | 5.81 | - | - | - | |
其他 Others | 2.49 | - | 2.77 | 8.30 | 0.72 | 0.83 | 7.69 | |
γ变形杆菌 γ-Proteobacteria | 78.61 | 35.82 | 40.71 | 34.44 | 61.51 | 45.83 | 56.78 | |
泛菌 Pantoea | 64.18 | 5.60 | 31.62 | 22.82 | 7.55 | 40.00 | 54.95 | |
假单胞菌 Pseudomonas | 13.43 | 27.99 | 5.93 | 2.07 | 13.31 | 3.75 | - | |
肠杆菌 Enterobacter | - | - | - | - | 17.63 | - | - | |
欧文氏菌 Erwinia | - | 1.12 | - | - | 9.35 | - | - | |
不动杆菌 Acinetobacter | - | 0.37 | - | - | 7.20 | - | - | |
其他 Others | 1.00 | 0.74 | 3.16 | 9.54 | 6.47 | 2.08 | 1.83 | |
放线菌 Actinobacteria | 1.00 | 1.49 | 24.90 | 15.77 | 5.76 | 8.75 | 4.03 | |
微杆菌 Microbacterium | - | 1.12 | 19.37 | 2.07 | - | 7.50 | 4.03 | |
短小杆菌 Curtobacterium | 1.00 | 0.37 | 5.53 | 10.79 | 5.76 | 1.25 | - | |
其他 Others | - | - | - | 2.90 | - | - | - | |
厚壁菌 Firmicutes | - | 57.84 | 0.40 | 3.32 | 29.50 | - | 0.37 | |
芽孢杆菌 Bacillus | - | 57.84 | - | - | 28.78 | - | - | |
其他 Others | - | - | 0.40 | 3.32 | 0.72 | - | 0.37 | |
拟菌 Bacteroidetes | 0.50 | - | 3.16 | 4.98 | - | 9.17 | 3.30 | |
鞘胺醇杆菌 Sphingobacterium | - | - | - | - | - | 7.08 | 1.47 | |
其他 Others | 0.50 | - | 3.16 | 4.98 | - | 2.08 | 1.83 | |
其他 Others | - | 1.49 | - | 2.90 | 1.08 | - | - |
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