Chin J Plant Ecol ›› 2012, Vol. 36 ›› Issue (8): 880-890.DOI: 10.3724/SP.J.1258.2012.00880

• Research Articles • Previous Articles     Next Articles

Diversity of indigenous bacterial communities in Oryza sativa seeds of different varieties

ZOU Yuan-Yuan1, LIU Lin1, LIU Yang1, ZHAO Liang1, DENG Qi-Yun2,*(), WU Jun2, ZHUANG Wen2, SONG Wei1,*()   

  1. 1College of Life Sciences, Capital Normal University, Beijing 100048, China
    2China National Hybrid Rice Research and Development Center, Changsha 410125, China
  • Received:2011-12-09 Accepted:2012-04-25 Online:2012-12-09 Published:2012-08-21
  • Contact: DENG Qi-Yun,SONG Wei

Abstract:

Aims Our purpose is to study the bacterial community structures in rice (Oryza sativa) seeds, and to compare and analyze community structures in different varieties in different hybrid combinations.
Methods Primers 799f and 1492r were used to amplify the bacterial 16S rDNA genes in the rice seeds. The community structure and diversity of indigenous bacteria in the rice seeds of hybrid progenies and their parental lines in three hybrid rice combinations, including ‘Fengyou 611’ (‘Fengyuan A’ × ‘Yuanhui 611’), ‘Jinyou 611’ (‘Jin 23A’ × ‘Yuanhui 611’) and ‘Jin 23A/09H013’ (‘Jin 23A’ × ‘09H013’), were analyzed by the 16S rDNA clone library technique and amplified by ribosomal DNA restriction analysis (ARDRA).
Important findings In seven clone libraries, each library had 200-300 positive clones and 30-40 operational taxonomic units (OTUs). Analysis for the representative sequences identified by ARDRA revealed diverse phyla of bacteria in the 16S rDNA libraries, which consisted of alpha, beta, and gamma subclasses of Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The dominant genera were Pantoea and Bacillus. Indigenous bacterial communities in the rice seeds of different varieties were different. The dominant bacteria in the hybrid progeny seeds had some relationship with that of their parental seeds in both variety and quantity. When the most abundant bacterial genera of both parental seeds were the same, such as Pantoea, the most abundant bacterial genus in the hybrid progeny seeds was also Pantoea, but with higher contents than that in the parents, as in the combination of ‘Fengyou 611’. When the most abundant bacterial genera in the two parental seeds were different and the abundance of the dominant bacterial genus in the male parent was < 50%, the dominant bacterial genus in the hybrid progeny seeds was the same as its female parental seeds, but with greater abundance, such as the combination ‘Jinyou 611’. However, when the abundance of the first dominant bacterial genus in the male parental seeds was > 50%, the most abundant bacterial genus in the hybrid progeny seeds was the same, and the abundance was lower than that in the male parental seeds, such as the combination ‘Jin 23A/09H013’.

Key words: community diversity, hybrid rice, indigenous bacteria, parental, progeny, seed-associated microbial ecology