Mitigation effects of exogenous glutathione on the growth of Dianthus chinensis seedlings under Cd stress

doi:10.3724/SP.J.1258.2013.00098

Abstract

Abstract:

Aims The objective was to investigate exogenous glutathione (GSH) mitigation effects of Cadmium (Cd) toxicity on ground cover plants.
Methods A greenhouse pot experiment was conducted to explore the effects of spraying different concentrations (0, 20, 40, 60, 80 and 100 mg·L ^-1) exogenous GSH on Dianthus chinensis seedlings exposed to 50 mg·kg ^-1 Cd in the soil.
Important findings 50 mg·kg ^-1 Cd significantly inhibited the growth of D. chinensis seedlings; however, a range of concentrations of sprayed exogenous GSH significantly relieved Cd stress on D. chinensis seedlings. Sprayed D. chinensis seedlings had increased activities of the catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), as well as increased ascorbic acid (AsA) and GSH contents, biomass, plant height and number of tillers. There were decreases in malondialdehyde (MDA), cell membrane permeability, Cd contents, O₂· ^- production rates and accumulated amount of H₂O₂. However, with increased exogenous GSH concentration, there was a downward trend in mitigation effects. Considering all factors, the mitigation effects were maximized by spraying 55-65 mg·L ^-1 exogenous GSH.

Key words: AsA-GSH cycle, Cd stress, Dianthus chinensis seedling, exogenous GSH, mitigation effect

DING Ji-Jun,PAN Yuan-Zhi,LI Li,LIU Shi-Liang,CUI Ming-Feng,GAO Pei-Gang. Mitigation effects of exogenous glutathione on the growth of Dianthus chinensis seedlings under Cd stress[J]. Chin J Plant Ecol, 2013, 37(10): 950-960.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2013.00098

https://www.plant-ecology.com/EN/Y2013/V37/I10/950

Figures/Tables 10

References 25

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处理 Treatment	生物量 Biomass (g DW·pot^-1)			株高 Plant height (cm)	分蘖数 Tiller number	抗性系数 Coefficient
处理 Treatment	地下部 Root	地上部 Shoot	整株 Whole plant	株高 Plant height (cm)	分蘖数 Tiller number	抗性系数 Coefficient
CK	1.89 ± 0.11^a	13.66 ± 0.30^a	15.56 ± 0.37^a	27.50 ± 2.38^a	11.8 ± 2.08^a	-
I	1.44 ± 0.05^b	10.59 ± 0.26^c	12.02 ± 0.21^d	17.37 ± 1.62^d	5.3 ± 1.53^b	0.77
II	1.44 ± 0.08^b	10.75 ± 0.38^c	12.18 ± 0.32^d	18.93 ± 0.26^cd	5.3 ± 2.52^b	0.78
III	1.59 ± 0.06^b	11.30 ± 0.29^b	12.90 ± 0.31^c	20.16 ± 2.15^c	7.7 ± 1.54^b	0.83
IV	1.53 ± 0.21^b	13.21 ± 0.14^a	14.73 ± 0.14^b	23.36 ± 0.42^b	8.3 ± 0.58^b	0.95
V	1.59 ± 0.08^b	11.34 ± 0.19^b	12.93 ± 0.26^c	19.31 ± 0.06^cd	6.7 ± 1.53^b	0.83
VI	1.49 ± 0.03^b	11.66 ± 0.27^b	13.15 ± 0.28^c	20.22 ± 0.20^c	7.3 ± 1.53^b	0.85

处理 Treatment	生物量 Biomass (g DW·pot^-1)			株高 Plant height (cm)	分蘖数 Tiller number	抗性系数 Coefficient
处理 Treatment	地下部 Root	地上部 Shoot	整株 Whole plant	株高 Plant height (cm)	分蘖数 Tiller number	抗性系数 Coefficient
CK	1.89 ± 0.11^a	13.66 ± 0.30^a	15.56 ± 0.37^a	27.50 ± 2.38^a	11.8 ± 2.08^a	-
I	1.44 ± 0.05^b	10.59 ± 0.26^c	12.02 ± 0.21^d	17.37 ± 1.62^d	5.3 ± 1.53^b	0.77
II	1.44 ± 0.08^b	10.75 ± 0.38^c	12.18 ± 0.32^d	18.93 ± 0.26^cd	5.3 ± 2.52^b	0.78
III	1.59 ± 0.06^b	11.30 ± 0.29^b	12.90 ± 0.31^c	20.16 ± 2.15^c	7.7 ± 1.54^b	0.83
IV	1.53 ± 0.21^b	13.21 ± 0.14^a	14.73 ± 0.14^b	23.36 ± 0.42^b	8.3 ± 0.58^b	0.95
V	1.59 ± 0.08^b	11.34 ± 0.19^b	12.93 ± 0.26^c	19.31 ± 0.06^cd	6.7 ± 1.53^b	0.83
VI	1.49 ± 0.03^b	11.66 ± 0.27^b	13.15 ± 0.28^c	20.22 ± 0.20^c	7.3 ± 1.53^b	0.85

指标 Index		PB	PH			NT	Cd_Root	Cd_Stem		Cd_Leaf	Con	MDA含量 MDA content		O₂·^-产生速率 O₂·^-production rate
r		0.523	0.446			0.417	-0.837	-0.899		-0.888	0.619	0.804		0.875
b		0.138*	0.027			0.021	-0.209^**	-0.083^**		-0.081^**	-0.075^**	-0.009^**		-0.027^**
a		12.293	18.562			5.730	54.481	15.510		14.786	31.240	5.486		5.477
指标 Index	H₂O₂含量 H₂O₂ content			POD活性 POD activity	SOD活性 SOD activity		CAT活性 CAT activity		APX活性 APX activity	MDAR活性 MDAR activity	DHAR活性 DHAR activity	GR活性 GR activity	AsA含量 AsA content		GSH含量 GSH content
r	0.840			0.417	0.122		0.721		0.204	0.672	0.462	0.637	0.565		0.757
b	-0.025^**			0.187	0.049		0.101^**		0.001	0.003^**	0.001	0.001^**	0.002^*		0.002^**
a	4.338			220.339	130.075		29.968		1.477	1.270	0.483	0.347	0.643		0.311

指标 Index		PB	PH			NT	Cd_Root	Cd_Stem		Cd_Leaf	Con	MDA含量 MDA content		O₂·^-产生速率 O₂·^-production rate
r		0.523	0.446			0.417	-0.837	-0.899		-0.888	0.619	0.804		0.875
b		0.138*	0.027			0.021	-0.209^**	-0.083^**		-0.081^**	-0.075^**	-0.009^**		-0.027^**
a		12.293	18.562			5.730	54.481	15.510		14.786	31.240	5.486		5.477
指标 Index	H₂O₂含量 H₂O₂ content			POD活性 POD activity	SOD活性 SOD activity		CAT活性 CAT activity		APX活性 APX activity	MDAR活性 MDAR activity	DHAR活性 DHAR activity	GR活性 GR activity	AsA含量 AsA content		GSH含量 GSH content
r	0.840			0.417	0.122		0.721		0.204	0.672	0.462	0.637	0.565		0.757
b	-0.025^**			0.187	0.049		0.101^**		0.001	0.003^**	0.001	0.001^**	0.002^*		0.002^**
a	4.338			220.339	130.075		29.968		1.477	1.270	0.483	0.347	0.643		0.311

指标 Index	Cd_root	Con	$\text{O}_{\text{2}}^{\overline{\bullet }}$	H₂O₂	POD	SOD	CAT	APX	MDAR	DHAR	GR	AsA	GSH
Cd_root	1
Con	0.70^**	1
O₂·^-	0.91^**	0.72^**	1
H₂O₂	0.72^**	0.52^*	0.72^**	1
POD	-0.52^*	-0.74^**	-0.56^**	-0.33	1
SOD	0.66^**	0.66^**	0.49^*	0.42	-0.50^*	1
CAT	-0.37	-0.10	-0.6^**	-0.42	0.14	0.27	1
APX	-0.11	-0.19	-0.35	0.08	0.39	0.03	0.47^*	1
MDAR	-0.85^**	-0.73^**	-0.84^**	-0.62^**	0.57^**	-0.40	0.48^*	0.19	1
DHAR	-0.71^**	-0.70^**	-0.76^**	-0.49^*	0.46^*	-0.33	0.45^*	0.29	0.78^**	1
GR	-0.78^**	-0.56^**	-0.84^**	-0.61^**	0.45^*	-0.28	0.59^**	0.24	0.72^**	0.83^**	1
AsA	-0.74^**	-0.66^**	-0.83^**	-0.48^*	0.55^**	-0.25	0.52^*	0.47^*	0.81^**	0.87^**	0.84^**	1
GSH	-0.49^*	-0.39	-0.65^**	-0.59^**	0.52^*	0.06	0.73^**	0.28	0.58^**	0.54^*	0.68^**	0.65^**	1