PHOTOSYNTHETIC PHYSIOLOGICAL RESPONSES OF GLYCYRRHIZA URALENSISUNDER DIFFERENT WATER AND NUTRIENT SUPPLIES IN KUBUQI DESERT, CHINA

doi:10.3773/j.issn.1005-264x.2009.06.011

Abstract

Abstract:

Aims Changes in precipitation pattern and increases in nitrogen deposition have been predicted in previous studies of global change, and these may significantly affect plant growth in terrestrial ecosystems and the availability of resources such as water and nutrients for plant use. Our objective was to determine photo-physiological responses and adaptation of licorice (Glycyrrhiza uralensis), a dominant legume species in the Kubuqi Desert, to different water and nutrient (N, P) supplies.

Methods Using a completely randomized design, we subjected second-year licorice seedlings to water treatment at four supplement levels (3/8, 1/2, 1, and 2 times mean annual precipitation of the study area) and four nutrient treatments (CK, N: 83mg N·kg^-1 soil, P: 92 mg P·kg^-1 soil, and NP: 83 mg N+92 mg P·kg^-1 soil) in 2005. We also determined diurnal changes of photo-physiological characteristics and light response curves.

Important findings Water supply and nutrient addition significantly affected licorice physiological characteristics. Water stress decreased gas exchange characteristics, such as net photosynthesis rate (P_n), stomatal conductance (G_s), and transpiration rate (T_r), and down-regulated several A-PAR relationship curve parameters, such as maximum net photosynthetic rate (P_max) and apparent quantum efficiency (AQE). The major control of photosynthesis under severe water shortage (W1) varied between stomatal and non-stomatal limitation. Therefore, the second-year licorice seedlings performed a quick physiological response and corresponding regulation to water supply. Nutrient addition could increase P_n, especially under high-water supply, and phosphorus addition could also stimulate P_max and light saturation point (LSP) and diminish light compensation point (LCP) under extremely low water supply. Shortage of water supply or nutrient addition combined with high water supply (W4) would decrease photosynthetic water use efficiency (PWUE). Our results suggest that water supply is the primary limiting factor and nutrient addition is secondary for licorice photosynthesis in this desert area.

Key words: Glycyrrhiza uralensis, photo-physiological responses, water supply, nutrient addition, Kubuqi Desert

LI Yang, HUANG Jian-Hui. PHOTOSYNTHETIC PHYSIOLOGICAL RESPONSES OF GLYCYRRHIZA URALENSISUNDER DIFFERENT WATER AND NUTRIENT SUPPLIES IN KUBUQI DESERT, CHINA[J]. Chin J Plant Ecol, 2009, 33(6): 1112-1124.

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Figures/Tables 6

References 54

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变异来源 Source of variation	自由度 df	净光合速率 P_n (μmol CO₂·m^-2·s^-1)	气孔导度 G_s (mmol H₂O·m^-2· s^-1)	胞间CO₂浓度 C_i (μmol· mol^-1)	光合水分利用效率 PWUE (μmol CO₂·mmol H₂O)	蒸腾速率 T_r (mmol H₂O·m^-2 ·s ^-1)	气孔限制值 L_s	最大净光合速率 P_max (μmol CO₂·m^-2 ·s ^-1)	表观量子效率 AQE (CO₂·photon^-1)	暗呼吸速率 R_d (μmol· m^-2·s^-1)	光补偿点 LCP (mmol· m^-2·s^-1)	光饱和点 LSP (mmol· m^-2·s^-1)
水分 Water	3	27.575^***	16.544^***	2.018	32.004^***	1.834	2.480	5.627^**	3.037^*	1.688	12.464^***	2.418
养分 Nutrient	3	0.387	0.880	0.726	1.371	0.499	0.683	8.977^***	2.852	2.219	2.889	13.789^***
水分×养分 Water×Nutrient	9	2.206^*	2.025	1.999	2.594^*	1.594	1.983	1.616	2.677^*	1.995	6.727^***	2.553^*

变异来源 Source of variation	自由度 df	净光合速率 P_n (μmol CO₂·m^-2·s^-1)	气孔导度 G_s (mmol H₂O·m^-2· s^-1)	胞间CO₂浓度 C_i (μmol· mol^-1)	光合水分利用效率 PWUE (μmol CO₂·mmol H₂O)	蒸腾速率 T_r (mmol H₂O·m^-2 ·s ^-1)	气孔限制值 L_s	最大净光合速率 P_max (μmol CO₂·m^-2 ·s ^-1)	表观量子效率 AQE (CO₂·photon^-1)	暗呼吸速率 R_d (μmol· m^-2·s^-1)	光补偿点 LCP (mmol· m^-2·s^-1)	光饱和点 LSP (mmol· m^-2·s^-1)
水分 Water	3	27.575^***	16.544^***	2.018	32.004^***	1.834	2.480	5.627^**	3.037^*	1.688	12.464^***	2.418
养分 Nutrient	3	0.387	0.880	0.726	1.371	0.499	0.683	8.977^***	2.852	2.219	2.889	13.789^***
水分×养分 Water×Nutrient	9	2.206^*	2.025	1.999	2.594^*	1.594	1.983	1.616	2.677^*	1.995	6.727^***	2.553^*

项目 Item	实验处理 Treatment	O	N	P	NP
净光合速率P_n (μmol CO₂·m^-2·s^-1)	W1	5.79±1.40^Aa	2.49±0.78^Aa	9.85±0.84^ABb	2.14±1.41^Aa
	W2	6.14±2.47^Aa	10.43±2.68^Ba	7.33±1.14^Aa	7.04±3.64A^Ba
	W3	13.65±0.63^Ba	18.92±0.35^Ba	13.44±2.98^BCa	13.34±2.87^Ca
	W4	16.72±0.96^Bb	12.19±1.72^Ba	15.55±0.42^Cb	20.59±0.37^Cc
气孔导度 G_s (mmol H₂O·m^-2 ·s ^-1)	W1	0.19±0.12^Aa	0.07±0.02^Aa	0.16±0.01^Aa	0.06±0.01^Aa
	W2	0.15±0.01^Aa	0.18±0.03^Ba	0.16±0.01^Ba	0.13±0.02^Ba
	W3	0.23±0.01^Aa	0.23±0.04^ABa	0.21±0.03^BCa	0.17±0.04^Ca
	W4	0.28±0.02^Aa	0.20±0.03^ABa	0.29±0.03^Ba	0.38±0.02^Ca
胞间CO₂浓度C_i (μmol·mol^-1)	W1	267.43±42.46^Aa	303.57±3.14^Aa	222.53±4.48^Aa	319.77±35.14^Aa
	W2	303.47±24.24^Aa	274.60±11.55^Aa	287.50±15.89^Aa	284.97±30.61^Aa
	W3	272.37±4.35^Aa	252.63±7.14^Aa	263.80±10.18^Aa	240.10±3.38^Aa
	W4	272.60±2.00^Aa	269.67±8.14^Aa	273.90±6.77^Aa	263.93±2.33^Aa
光合水分利用效率PWUE (μmol CO₂·mmol H₂O)	W1	1.57±0.51^Aa	1.18±0.05^Aa	2.86±0.09^Ba	1.88±0.69^Aa
	W2	1.54±0.58^Aa	2.13±0.24^Ba	1.75±0.36^Aa	1.73±0.69^Aa
	W3	2.85±0.14^Aa	4.23±0.81^Ca	2.71±0.32^Ba	3.13±0.08^Ba
	W4	5.16±0.23^Bc	4.25±0.09^Db	3.55±0.17^Ba	3.57±0.11^Ba
蒸腾速率T_r (mmol H₂O·m^-2·s^-1)	W1	3.91±0.74^Aa	2.10±0.63^Aa	3.45±0.31^Aa	1.89±0.35^Aa
	W2	3.91±0.21^Aa	4.75±0.83^Aa	4.28±0.29^Aa	3.60±0.59^Aa
	W3	4.80±0.05^Aa	4.75±0.73^Aa	4.87±0.53^Aa	4.25±0.88^Aa
	W4	3.25±0.19^Aa	2.87±0.42^Aa	4.41±0.35^Aa	5.78±0.19^Aa
气孔限制值L_s	W1	0.30±0.11^Aa	0.20±0.01^Aa	0.42±0.01^Aa	0.17±0.09^Aa
	W2	0.21±0.06^Aa	0.29±0.03^Aa	0.25±0.04^Aa	0.25±0.08^Aa
	W3	0.30±0.01^Aa	0.35±0.02^Aa	0.32±0.03^Aa	0.38±0.01^Aa
	W4	0.30±0.01^Aa	0.31±0.02^Aa	0.30±0.02^Aa	0.32±0.01^Aa

项目 Item	实验处理 Treatment	O	N	P	NP
净光合速率P_n (μmol CO₂·m^-2·s^-1)	W1	5.79±1.40^Aa	2.49±0.78^Aa	9.85±0.84^ABb	2.14±1.41^Aa
	W2	6.14±2.47^Aa	10.43±2.68^Ba	7.33±1.14^Aa	7.04±3.64A^Ba
	W3	13.65±0.63^Ba	18.92±0.35^Ba	13.44±2.98^BCa	13.34±2.87^Ca
	W4	16.72±0.96^Bb	12.19±1.72^Ba	15.55±0.42^Cb	20.59±0.37^Cc
气孔导度 G_s (mmol H₂O·m^-2 ·s ^-1)	W1	0.19±0.12^Aa	0.07±0.02^Aa	0.16±0.01^Aa	0.06±0.01^Aa
	W2	0.15±0.01^Aa	0.18±0.03^Ba	0.16±0.01^Ba	0.13±0.02^Ba
	W3	0.23±0.01^Aa	0.23±0.04^ABa	0.21±0.03^BCa	0.17±0.04^Ca
	W4	0.28±0.02^Aa	0.20±0.03^ABa	0.29±0.03^Ba	0.38±0.02^Ca
胞间CO₂浓度C_i (μmol·mol^-1)	W1	267.43±42.46^Aa	303.57±3.14^Aa	222.53±4.48^Aa	319.77±35.14^Aa
	W2	303.47±24.24^Aa	274.60±11.55^Aa	287.50±15.89^Aa	284.97±30.61^Aa
	W3	272.37±4.35^Aa	252.63±7.14^Aa	263.80±10.18^Aa	240.10±3.38^Aa
	W4	272.60±2.00^Aa	269.67±8.14^Aa	273.90±6.77^Aa	263.93±2.33^Aa
光合水分利用效率PWUE (μmol CO₂·mmol H₂O)	W1	1.57±0.51^Aa	1.18±0.05^Aa	2.86±0.09^Ba	1.88±0.69^Aa
	W2	1.54±0.58^Aa	2.13±0.24^Ba	1.75±0.36^Aa	1.73±0.69^Aa
	W3	2.85±0.14^Aa	4.23±0.81^Ca	2.71±0.32^Ba	3.13±0.08^Ba
	W4	5.16±0.23^Bc	4.25±0.09^Db	3.55±0.17^Ba	3.57±0.11^Ba
蒸腾速率T_r (mmol H₂O·m^-2·s^-1)	W1	3.91±0.74^Aa	2.10±0.63^Aa	3.45±0.31^Aa	1.89±0.35^Aa
	W2	3.91±0.21^Aa	4.75±0.83^Aa	4.28±0.29^Aa	3.60±0.59^Aa
	W3	4.80±0.05^Aa	4.75±0.73^Aa	4.87±0.53^Aa	4.25±0.88^Aa
	W4	3.25±0.19^Aa	2.87±0.42^Aa	4.41±0.35^Aa	5.78±0.19^Aa
气孔限制值L_s	W1	0.30±0.11^Aa	0.20±0.01^Aa	0.42±0.01^Aa	0.17±0.09^Aa
	W2	0.21±0.06^Aa	0.29±0.03^Aa	0.25±0.04^Aa	0.25±0.08^Aa
	W3	0.30±0.01^Aa	0.35±0.02^Aa	0.32±0.03^Aa	0.38±0.01^Aa
	W4	0.30±0.01^Aa	0.31±0.02^Aa	0.30±0.02^Aa	0.32±0.01^Aa

项目 Item	实验处理 Treatment	CK	N	P	NP
最大净光合速率 P_max (μmol CO₂·m^-2 ·s ^-1)	W1	7.44±2.32^Aa	4.65±1.56^Aa	9.94±2.25^Aa	3.59±1.09^Aa
	W2	9.16±1.75^Aa	6.68±1.81^Aa	15.73±2.56^Ab	6.34±1.20^ABa
	W3	13.93±1.57^Ab	6.55±0.71^Aa	13.60±0.59^Ab	8.07±0.90^BCa
	W4	13.13±4.05^Aa	10.22±0.84^Aa	10.10±0.35^Aa	11.07±0.73^Ca
表观量子效率 AQE (CO₂·photon^-1)	W1	0.0375±0.0107^Aa	0.0266±0.0024^Aa	0.0382±0.0032^Aa	0.0259±0.0033^Aa
	W2	0.0444±0.0125^Aa	0.0575±0.0060^Ba	0.0408±0.0033^Aa	0.0327±0.0046^Aa
	W3	0.0320±0.0084^Aa	0.0616±0.0074^Ba	0.0400±0.0093^Aa	0.0500±0.0092^Aa
	W4	0.0520±0.0028^Ab	0.0485±0.0104^ABb	0.0194±0.0010^Aa	0.0448±0.0025^Ab
暗呼吸速率 R_d (μmol·m^-2·s^-1)	W1	-2.99±0.99^Aa	-1.63±0.30^Aa	-1.74±0.22^Aa	-1.17±0.25^Aa
	W2	-1.56±0.43^Aa	-2.00±0.20^Aa	-2.37±0.48^Aa	-1.61±0.13^Aa
	W3	-1.91±0.32^Aa	-2.02±0.33^Aa	-1.22±0.26^Aa	-1.58±0.21^Aa
	W4	-1.37±0.15^Aa	-1.92±0.19^Aa	-0.72±0.09^Aa	-1.68±0.09^Aa
光补偿点 LCP (mmol·m^-2·s^-1)	W1	76.60±7.50^Bb	60.30±5.93^Bab	45.73±5.00^ABa	44.37±5.72^ABa
	W2	37.13±5.25^Aa	36.47±7.68^Aa	57.10±6.66^Ba	50.13±2.82^Ba
	W3	63.30±7.08^Bb	32.57±1.50^Aa	31.60±3.64^Aa	32.53±3.64^Aa
	W4	26.47±3.07^Aa	41.97±6.43^Ba	36.73±2.62^Aa	37.50±1.16^Aa
光饱和点 LSP (mmol·m^-2·s^-1)	W1	280.00±21.17^Aa	226.67±51.61^Aa	301.00±50.39^Aa	177.00±38.22^Aa
	W2	285.00±65.25^Aab	162.00±56.03^Aa	437.33±35.63^Ab	247.00±37.40^Aa
	W3	544.67±92.96^Bb	144.67±23.67^Aa	427.67±123.52^Aab	213.33±57.48^Aa
	W4	273.67±60.92^Aa	271.67±54.14^Aa	559.00±29.70^Ab	266.00±32.08^Aa