ISOLATION AND IDENTIFICATION OF ALLELOCHEMICALS FROM REHMANNIA GLUTINOSA THAT AFFECT SESAMUM INDICUM

doi:10.3773/j.issn.1005-264x.2009.06.020

Abstract

Abstract:

Aims Our objective was to explore why Sesamum indicum yield declined severely when planted after Rehmannia glutinosa in crop rotation.

Methods We used extraction, silica gel column chromatography, gas chromatography mass spectrometry and bioassay to isolate and identify allelochemicals from R. glutinosa that affect S. indicum.Bioassays were conducted with extractions from different parts of R. glutinosa.

Important findings Leaf parts had high allelochemical content. Ethyl acetate phase extraction contained most allelochemicals. Active elution Fr7 was obtained by silica gel column chromatography. Allelochemicals lauric acid and 2,6-Ditertbutyl phenol were isolated and identified by use of gas chromatography mass spectrometry. R. glutinosa and S. indicum both have serious continuous cropping obstacles. Allelochemicals affecting S. indicum are different from toxic materials of R. glutinosa. Additional research is needed to further explore interactions of allelochemicals with soil and effects on S. indicumand on the possibility of reducing the effect of R. glutinosa on S. indicum.

Key words: Rehmannia glutinosa, Sesamum indicum, allelopathy, allelochemicals, isolation and identification

WANG Ming-Dao, CHEN Hong-Ge, LIU Xin-Yu, GAO Yu-Qian, WU Kun, JIA Xin-Cheng. ISOLATION AND IDENTIFICATION OF ALLELOCHEMICALS FROM REHMANNIA GLUTINOSA THAT AFFECT SESAMUM INDICUM[J]. Chin J Plant Ecol, 2009, 33(6): 1191-1198.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.06.020

https://www.plant-ecology.com/EN/Y2009/V33/I6/1191

Figures/Tables 6

References 26

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部位 Part	浓度 Concentration (mg·mL^-1)	发芽率 Germination rate (%)	苗高 Seedling height (cm)	根长 Root length (cm)	鲜重 Fresh weight (g)
对照 CK		92.0±3.6^aA	2.74±0.26^aA	5.28±0.28^aA	0.277±0.018^aA
根 Root	10	93.3±3.1^aA	2.78±0.13^aA	5.46±0.29^aA	0.283±0.005^aA
	50	90.7±3.1^abA	2.64±0.21^aA	4.98±0.34^bA	0.242±0.013b^AB
	100	84.0±4.6^bA	2.22±0.24^bB	4.24±0.27^cB	0.215±0.017^cB
茎 Stem	10	90.3±3.5^aA	2.61±0.25^aA	5.16±0.27^aA	0.266±0.019^abA
	50	86.7±3.1^abA	2.41±0.24^aAB	4.66±0.23^bB	0.251±0.022^abA
	100	83.7±2.1^bA	2.05±0.22^bB	4.09±0.18^cC	0.232±0.01b^A
叶 Leaf	10	88.0±6.6^aA	2.46±0.17^bAB	4.16±0.23^bB	0.195±0.018^bB
	50	82.0±3.6^aAB	2.14±0.17^cB	2.79±0.17^cC	0.158±0.016^cBC
	100	69.0±6.1^bB	1.64±0.16^dC	2.05±0.15^dD	0.114±0.016^dC

部位 Part	浓度 Concentration (mg·mL^-1)	发芽率 Germination rate (%)	苗高 Seedling height (cm)	根长 Root length (cm)	鲜重 Fresh weight (g)
对照 CK		92.0±3.6^aA	2.74±0.26^aA	5.28±0.28^aA	0.277±0.018^aA
根 Root	10	93.3±3.1^aA	2.78±0.13^aA	5.46±0.29^aA	0.283±0.005^aA
	50	90.7±3.1^abA	2.64±0.21^aA	4.98±0.34^bA	0.242±0.013b^AB
	100	84.0±4.6^bA	2.22±0.24^bB	4.24±0.27^cB	0.215±0.017^cB
茎 Stem	10	90.3±3.5^aA	2.61±0.25^aA	5.16±0.27^aA	0.266±0.019^abA
	50	86.7±3.1^abA	2.41±0.24^aAB	4.66±0.23^bB	0.251±0.022^abA
	100	83.7±2.1^bA	2.05±0.22^bB	4.09±0.18^cC	0.232±0.01b^A
叶 Leaf	10	88.0±6.6^aA	2.46±0.17^bAB	4.16±0.23^bB	0.195±0.018^bB
	50	82.0±3.6^aAB	2.14±0.17^cB	2.79±0.17^cC	0.158±0.016^cBC
	100	69.0±6.1^bB	1.64±0.16^dC	2.05±0.15^dD	0.114±0.016^dC

有机相 Organic phase	浓度 Concentration (mg·mL^-1)	发芽率 Germination rate (%)	苗高 Seedling height (cm)	根长 Root length (cm)	鲜重 Fresh weight (g)
对照 CK		91.7±3.2^aA	2.71±0.21^aA	5.30±0.17^aA	0.27±0.015^aA
石油醚相 Petroleum-ether phase	100	90.0±5.66^aA	2.66±0.24^aA	5.28±0.13^aA	0.27±0.022^aA
二氯甲烷相 Dichloromethane phase	5	89.0±2.6^abA	2.67±0.11^aAB	5.12±0.22^abAB	0.27±0.014^aA
	10	86.0±3.0^abA	2.60±0.21^aAB	4.82±0.28^bB	0.25±0.013^aAB
	20	83.7±3.2^bA	2.32±0.20^bB	4.16±0.25^cC	0.21±0.022^bB
乙酸乙酯相 Ethyl-acetate phase	5	83.3±3.8^bA	2.45±0.16^bB	4.24±0.12^bB	0.219±0.023^bB
	10	74.0±2.6^cB	2.04±0.12^cC	3.16±0.29^cC	0.154±0.014^cC
	20	64.0±3.6^dC	1.66±0.13^dD	1.87±0.20^dD	0.109±0.02^dC
正丁醇相 n-Butanol phase	5	86.7±3.2^ab	2.70±0.13^aA	5.21±0.11^aA	0.26±0.016^aA
	10	83.3±2.1^b	2.56±0.19^aAB	4.94±0.24^bA	0.24±0.013^aAB
	20	76.7±4.2^c	2.29±0.16^bB	4.28±0.22^cB	0.21±0.017^bB

有机相 Organic phase	浓度 Concentration (mg·mL^-1)	发芽率 Germination rate (%)	苗高 Seedling height (cm)	根长 Root length (cm)	鲜重 Fresh weight (g)
对照 CK		91.7±3.2^aA	2.71±0.21^aA	5.30±0.17^aA	0.27±0.015^aA
石油醚相 Petroleum-ether phase	100	90.0±5.66^aA	2.66±0.24^aA	5.28±0.13^aA	0.27±0.022^aA
二氯甲烷相 Dichloromethane phase	5	89.0±2.6^abA	2.67±0.11^aAB	5.12±0.22^abAB	0.27±0.014^aA
	10	86.0±3.0^abA	2.60±0.21^aAB	4.82±0.28^bB	0.25±0.013^aAB
	20	83.7±3.2^bA	2.32±0.20^bB	4.16±0.25^cC	0.21±0.022^bB
乙酸乙酯相 Ethyl-acetate phase	5	83.3±3.8^bA	2.45±0.16^bB	4.24±0.12^bB	0.219±0.023^bB
	10	74.0±2.6^cB	2.04±0.12^cC	3.16±0.29^cC	0.154±0.014^cC
	20	64.0±3.6^dC	1.66±0.13^dD	1.87±0.20^dD	0.109±0.02^dC
正丁醇相 n-Butanol phase	5	86.7±3.2^ab	2.70±0.13^aA	5.21±0.11^aA	0.26±0.016^aA
	10	83.3±2.1^b	2.56±0.19^aAB	4.94±0.24^bA	0.24±0.013^aAB
	20	76.7±4.2^c	2.29±0.16^bB	4.28±0.22^cB	0.21±0.017^bB

组分 Fraction	浓度 Concentration (mg·mL^-1)	发芽率 Germination rate (%)	苗高 Seedling height (cm)	根长 Root length (cm)	鲜重 Fresh weight (g)
对照 CK		91.3±3.5^aA	2.70±0.20^aA	5.30±0.15^aA	0.272±0.016^aA
Fr6	5	88.0±3.0^abA	2.65±0.09^aA	5.12±0.21^aAB	0.265±0.014^abAB
	10	85.0±3.0^abA	2.54±0.18^abA	4.81±0.18^bBC	0.245±0.010^bcAB
	20	83.0±4.4^bA	2.39±0.15^bA	4.58±0.22^bC	0.232±0.008^cB
Fr7	5	82.3±2.5^bB	2.46±0.17^bA	4.58±0.22^bB	0.234±0.022^bA
	10	72.7±3.1^cC	2.06±0.15^cB	3.20±0.20^cB	0.177±0.021^cB
	20	64.7±2.3^dD	1.72±0.10^dC	1.62±0.22^dD	0.093±0.006^dC
Fr8	5	86.7±3.2^abA	2.51±0.16^abA	5.10±0.19^aA	0.264±0.015^aA
	10	84.3±2.5^bAB	2.42±0.16^bAB	4.39±0.27^bB	0.226±0.024^bAB
	20	76.0±3.6^cB	2.1±0.18^cB	3.74±0.22^cC	0.197±0.021^bB