EFFECTS OF CALCIUM NITRATE STRESS ON ASCORBATE-GLUTATHIONE CYCLE METABOLISM IN LEAVES OF HYDROPONICALLY-GROWN GRA- FTED EGGPLANT SEEDLINGS

doi:10.3773/j.issn.1005-264x.2008.05.007

Abstract

Abstract:

Aims Oxidative stress is one of the responses of plants to salt stress, which causes the production of hydrogen peroxide (H₂O₂). Plants grafted on salt resistant rootstocks could improve their tolerance to salt stress because of the vigorous root system of rootstocks. However, little is known about the relationship between salt tolerance of grafted plants and the scavenging system of H₂O₂ under Ca(NO₃)₂ stress. Our objective was to investigate the mechanism and roles of ascorbate-glutathione metabolism system in salt tolerance of grafted eggplant under Ca(NO₃)₂ stress, including ascorbate pool, glutathione pool and the activities of antioxidant enzymes.

Methods We grafted cultivar ‘Suqi qie’ ( Solanum melongena) onto rootstock of ‘Torvum Vigor’ ( S. torvum), which is a salt tolerant cultivar introduced from Japan. We compared leaves of hydroponically-grown grafted and own-root eggplant seedlings grown under 80 mmol·L^-1Ca(NO₃)₂ stress for changes of antioxidant enzyme activities, contents of antioxidants in ascorbate-glutathione cycle metabolism and content of H₂O₂.

Important findings Under Ca(NO₃)₂ stress, H₂O₂ content increased in leaves of eggplant seedlings and was significantly lower in leaves of grafted seedlings than own-root seedlings. Also, activities of antioxidant enzymes (APX, DHAR and GR), regenerating rates of AsA and GSH and redox statuses (ratios of AsA/DHA and GSH/GSSG) were significantly higher in leaves of grafted seedlings than own-root seedlings. We concluded that grafted eggplant seedlings had efficient metabolism of ascorbate-glutathione cycle, which scavenged the H₂O₂ rapidly to alleviate the oxidative damage of Ca(NO₃)₂ stress; therefore, grafted seedlings had a stronger tolerance to salt stress.

Key words: grafted eggplant, Ca(NO₃)₂ stress, ascorbate-glutathione cycle, hydroponically-grown

WEI Guo-Ping, ZHU Yue-Lin, LIU Zheng-Lu, ZHANG Gu-Wen, YANG Li-Fei. EFFECTS OF CALCIUM NITRATE STRESS ON ASCORBATE-GLUTATHIONE CYCLE METABOLISM IN LEAVES OF HYDROPONICALLY-GROWN GRA- FTED EGGPLANT SEEDLINGS[J]. Chin J Plant Ecol, 2008, 32(5): 1023-1030.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2008.05.007

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Figures/Tables 4

Fig.1 Effects of Ca(NO3)2 stress on APX, DHAR and GR activities in leaves of grafted and own-root eggplant seedlings

Fig. 2 Effects of Ca(NO3)2 stress on AsA and DHA contents and AsA/DHA ratio in leaves of grafted and own-root eggplant seedlings

Fig. 3 Effects of Ca(NO3)2 stress on GSH and GSSG contents and GSH/GSSG ratio in leaves of grafted and own-root eggplant seedlings

Table 1 Effect of Ca(NO3)2 stress on H2O2 content (μmol·g-1FW) in leaves of grafted and own-root eggplant seedlings

处理 Treatment	胁迫时间 Stress time (d)
处理 Treatment	0	3	6	9	12	15
O1	5.08±0.22^a	5.22±0.20^a	5.07±0.30^b	5.08±0.30^c	5.14±0.24^c	5.24±0.27^c
O2	5.04±0.37^a	5.48±0.70^a	6.48±0.17^a	8.51±0.35^a	11.71±1.46^a	13.92±1.19^a
G1	4.82±0.24^a	4.83±0.21^a	5.03±0.14^b	5.17±0.10^c	5.20±0.19^c	5.10±0.24^c
G2	5.05±0.19^a	4.90±0.19^a	5.38±0.09^b	5.97±0.21^b	6.93±0.23^b	8.22±0.34^b

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