EFFECTS OF TEMPERATURE AND LIGHT TREATMENT ON VIOLAXANTHIN DE-EPOXIDASE ACTIVITY AND XANTHOPHYLL CYCLE-DEPENDENT ENERGY DISSIPATION IN WHEAT LEAVES

doi:10.3773/j.issn.1005-264x.2008.05.006

Abstract

Abstract:

Aims Our objective was to explore how temperature and light directly affect activity of violaxanthin de-epoxidase (VDE) in vitro and to clarify the relationship between VDE activity and xanthophyll cycle-dependant energy dissipation.

Methods We investigated VDE activities and energy dissipation in wheat leaves treated with different temperatures (4, 25, 38 and 45 ℃) combined with different light intensities (200, 500, 900 and 1 200 μmol·m ^-2·s^-1).

Important findings Maximum activity of VDE in wheat leaves appeared at 30 ℃, suggesting that this was the optimum temperature for VDE in vitro. Light intensity had no effects on VDE activity in wheat leaves in vitro. Compared with 25 ℃, 4 ℃ treatment exhibited no obvious effect on VDE activity; however, 38 ℃ treatment caused slight increase while 45 ℃ caused dramatic decrease in the VDE activity. With increasing light intensity, non-photochemical quenching (NPQ) and xanthophyll cycle-dependent energy dissipation (q_E) increased significantly. However, the ratio q_E/NPQ decreased slightly when the temperature increased from 4 ℃ to 38 ℃ and decreased significantly when the temperature was increased to 45 ℃. Under low and moderate light intensity, the VDE activity measured at treatment temperature was in agreement with the q_E/NPQ, which is a measure of the capacity of xanthophyll cycle-dependent energy dissipation.

Key words: xanthophyll cycle, violaxanthin de-epoxidase (VDE), photoinhibition, energy dissipation

CHEN Hua-Xin, CHEN Wei, JIANG Chuang-Dao, GAO Hui-Yuan, ZOU Qi. EFFECTS OF TEMPERATURE AND LIGHT TREATMENT ON VIOLAXANTHIN DE-EPOXIDASE ACTIVITY AND XANTHOPHYLL CYCLE-DEPENDENT ENERGY DISSIPATION IN WHEAT LEAVES[J]. Chin J Plant Ecol, 2008, 32(5): 1015-1022.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2008.05.006

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Figures/Tables 6

Fig. 1 Effect of various temperatures on violaxanthin de-epoxidase (VDE) activities

Fig. 2 Response of actual photochemical efficiency of PSⅡ (ΦPSⅡ ) to light intensity at different temperatures (a) and to temperatures under different light conditions (b)

Fig. 3 Response of maximum photochemical efficiency of PSⅡ (Fv/Fm ) to light intensity at different temperatures (a) and to temperatures under different light conditions (b) Notes see fig.2

Fig. 4 Response of non-photochemical quench NPQ, energy dissipation depending on xanthophyll cycle qEto light intensity at different temperatures (a, c) and to temperature under different light conditions (b, d)

Fig. 5 Response of qE/NPQ in wheat leaves to light intensities at different temperatures (a) and temperatures at different light conditions (b). Samples were exposed to different light intensity and temperature for 4 h ( means ± SE, n=5 to 6)

Fig. 6 Response of violaxanthin de-epoxidase (VDE) activities to light intensity at different temperatures (a、c) and temperatures at different light conditions (b、d)

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