NaCl stress aggravates photoinhibition of photosystem II and photosystem I in Capsicum annuum leaves under high irradiance stress

doi:10.3724/SP.J.1258.2011.00681

Abstract

Abstract:

Aims Our aim was to study effects of salt stress and high irradiance on photoinhibition of photosystem II (PSII) and I (PSI) in leaves of sweet pepper (Capsicum annuum).

Methods We used the chlorophyll a fluorescence technique and the JIP-test to study the responses of sweet pepper leaves to salt stress with different NaCl concentrations and under high irradiance (1 000 μmol·m^-2·s^-1).

Important findings The chlorophyll a fluorescence induction curves changed greatly as induced by NaCl stress under high irradiance, and photoinhibition of PSII was aggravated with apparent effects on PSII reaction centers and the acceptor side. Also, the donor side of PSII was damaged by high NaCl concentration under high irradiance. Additionally, the activity of PSI reaction centers (P700⁺) was inhibited by NaCl stress under high irradiance. Therefore, NaCl stress could aggravate photoinhibition of sweet pepper photosystem under high irradiance, and photoinhibition was greater with higher NaCl concentration. However, PSI photoinhibition was less than PSII photoinhibition. Additionally, high NaCl concentration could induce both damage to PSII donor sides and PSI.

Key words: high irradiance stress, photoinhibition, photosystem, salt stress, sweet pepper

SONG Xu-Li, HU Chun-Mei, MENG Jing-Jing, HOU Xi-Lin, HE Qi-Wei, LI Xin-Guo. NaCl stress aggravates photoinhibition of photosystem II and photosystem I in Capsicum annuum leaves under high irradiance stress[J]. Chin J Plant Ecol, 2011, 35(6): 681-686.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00681

https://www.plant-ecology.com/EN/Y2011/V35/I6/681

Figures/Tables 4

Fig. 1 Effects of NaCl stress on the chlorophyll a fluorescence transient curve in sweet pepper leaves under high irradiance. The data of T250 was on the 3rd day, and the data of the other stresses were on the 7th day. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

Fig. 2 Effects of NaCl stress on φP0 (A), VK (B), Vj (C) andΨ0 (D) in sweet pepper leaves under high irradiance (mean ± SD). φPo, maximum quantum yield for primary photochemistry; Ψ0, the efficiency by which a absorbed photon in the antennae moves an electron into the electron transport chain beyond QA-; Vj, relative variable fluorescence at the J-step; Vk, relative variable fluorescence intensity at 300 μs. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

Fig. 3 Effects of NaCl stress on RC/CSm (A), ABS/CSm (B), TR/CSm (C) and ET/CSm (D) in sweet pepper leaves under high irradiance (mean ± SD). ABS/CSm, absorption flux per excited cross section; ET/CSm, electron transport flux per cross section; RC/CSm, density of PSII active reaction centers per excited cross-section; TR/CSm, trapped energy flux per cross section. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

Fig. 4 Effects of NaCl stress on the activity of PSI (P700+) in sweet pepper leaves under high irradiance (mean ± SD). T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

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