Exogenous polyamines alleviating salt stress on peanuts (Arachis hypogaea) grown in pots

doi:10.17521/cjpe.2015.0117

Abstract

Abstract:

Aims Soil salinity is a major limiting factor for plant establishment, development and productivity. In recent years, the contradiction between oil crops and food crops for land is increasingly prominent. In order not to take up the land for food, peanut planting on saline-alkali land could be a promising option. However, peanuts have been rarely grown in saline-alkali land, which may be due to the reduction of peanut yield caused by salt stress. Therefore, research of peanut salt resistance has important practical significance.Methods In order to investigate the effects of exogenous polyamines on peanut (Arachis hypogaea) grown in pots under salt stress, ‘Huayu 22’, one of the peanut cultivars, was used as materials by being foliar-sprayed with 1 mmol·L^-1 putrescine (Put), 1 mmol·L^-1 spermidine (Spd) and 1 mmol·L^-1 spermine (Spm) to elucidate the role of exogenous polyamines on peanuts under 150 mmol·L^-1NaCl. Important findingsResults showed that growth, yield, chlorophyll contents and antioxidant enzyme activities of peanut seedling decreased, however, malondialdehyde (MDA) content and relative electrolytic leakage increased under salt stress. Meanwhile, exogenous polyamines significantly improved the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduced the relative electrolytic leakage and MDA content in peanut leaves under salt stress and thus alleviating the oxidative damage of salt stress on plasma membrane. It is obvious that exogenous polyamines could improve chlorophyll contents, plant height, number of branch and the amount of dry matter accumulation, even pod yield under salt stress. Among these three polyamines, the effects of exogenous Spm on alleviating salt stress were most effective These results showed that exogenous polyamines, especially Spm, were favorable for the seedlings to increase reactive oxygen metabolism and photosynthesis, which improved peanut growth and reduced the inhibitory effects of salt stress on peanuts.

Key words: antioxidant enzyme, peanut, polyamines, salt stress, yield

MENG De-Yun,HOU Lin-Lin,YANG Sha,MENG Jing-Jing,GUO Feng,LI Xin-Guo,WAN Shu-Bo. Exogenous polyamines alleviating salt stress on peanuts (Arachis hypogaea) grown in pots[J]. Chin J Plan Ecolo, 2015, 39(12): 1209-1215.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2015.0117

https://www.plant-ecology.com/EN/Y2015/V39/I12/1209

Figures/Tables 6

Table 1 Effects of exogenous polyamines on agronomic characters of Arachis hypogaea seedlings under salt stress (mean ± SD)

处理 Treatment	主茎高 Plant height (cm)	侧枝长 Branch length (cm)	分枝数 Branch number
CK	31.33 ± 0.63^a	32.17 ± 0.31^a	14.33 ± 0.24^a
NaCl	26.50 ± 0.25^c	27.00 ± 0.48^b	9.67 ± 0.24^c
NaCl + Put	27.67 ± 0.29^bc	30.50 ± 0.85^ab	11.67 ± 0.47^b
NaCl + Spd	29.33 ± 0.52^b	29.25 ± 0.64^ab	12.67 ± 0.24^ab
NaCl + Spm	29.67 ± 0.58^b	31.50 ± 0.38^a	11.67 ± 0.24^b

Fig. 1 Effects of exogenous polyamines on total biomass of Arachis hypogaea under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1.

Fig. 2 Effects of exogenous polyamines on leaf chlorophyll content of Arachis hypogaea under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1.

Fig. 3 Effects of exogenous polyamines on superoxide dismutase (SOD) activity (A), peroxidase (POD) activity (B) and catalse (CAT) activity (C) of Arachis hypogaea leaves under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1.

Fig. 4 Effects of exogenous polyamines on relative electrolytic leakage (A) and malondialdehyde (MDA) content (B) of Arachis hypogaea leaves under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1.

Table 2 Effects of exogenous polyamines on the yield of Arachis hypogaea under salt stress (mean ± SD)

处理 Treatment	产量 Pods mass per plant (g)	饱果数 Full fruits number per plant	总果数 Total pod number per plant	果针数 Gynophores number per plant
CK	47.57 ± 1.59^a	29.67 ± 0.67^a	39.70 ± 1.33^a	67.00 ± 2.00^a
NaCl	35.50 ± 0.93^c	16.21 ± 0.33^c	30.67 ± 2.67^b	40.67 ± 1.48^d
NaCl + Put	39.17 ± 0.87^bc	17.67 ± 0.33^bc	31.00 ± 0.67^b	43.00 ± 2.67^cd
NaCl + Spd	40.25 ± 1.13^b	21.00 ± 1.24^b	31.67 ± 0.48^b	45.00 ± 1.67^c
NaCl + Spm	41.50 ± 1.32^b	20.67 ± 0.67^b	39.33 ± 1.24^a	57.00 ± 3.33^b

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