Comparison of chlorophyll content and fluorescence parameters of six pasture species in two habitats in China

doi:10.3724/SP.J.1258.2011.00672

Abstract

Abstract:

Aims Qianshan Pasture in the Kunlun Mountains and Cele Oasis of China are different habitats with different elevations. We compared six pasture species in terms of chlorophyll (Chl) content and fluorescence parameters to investigate the effect of habitat on photosystem II (PSII).

Methods Agropyron cristatum, Bromus inermis, Festuca elata, Elymus dahuricus, Onobrychis pulchella and Medicago sativa var. luxurians, were grown in both sites. Leaf pigment content was measured by 95% ethanol method, and PSII chlorophyll fluorescence parameters were monitored by using a Plant Efficiency Analyzer (PEA).

Important findings The measured physiological parameters differed greatly in all six species growing in the two habitats. In Qianshan Pasture, all species showed obviously higher Chl a, Chl b and total Chl content, while those growing in Cele Oasis had higher Chl a/Chl b. Plants in Kunlun Mountains also had markedly higher maximum fluorescence yield (F_m), maximum photochemical efficiency of PSII (F_v/F_m), potential activity of PSII (F_v/F_o) and active reaction centers per cross-section (RC/CS_o), as well as lower minimum fluorescence yield (F_o), absorption flux per reaction center (ABC/RC), maximal trapping flux per reaction center (TR_o/RC), flux of dissipated excitation energy per reaction center (DI_o/RC) and initial slope of fluorescence intensity (M_o) than those in the Cele Oasis. These parameters also showed large differences in variation amplitude between habitats. The results indicated that environmental conditions in the Cele Oasis were stressful to pasture species. It decreased chlorophyll content and inactivated PSII. The habitat in Kunlun Mountains was relatively favorable for these species.

Key words: chlorophyll, chlorophyll fluorescence, habitat, pasture

LI Lei, LI Xiang-Yi, LIN Li-Sha, WANG Ying-Ju, XUE Wei. Comparison of chlorophyll content and fluorescence parameters of six pasture species in two habitats in China[J]. Chin J Plant Ecol, 2011, 35(6): 672-680.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00672

https://www.plant-ecology.com/EN/Y2011/V35/I6/672

Figures/Tables 5

Fig. 1 Changes of pigment content of pasture in two habitats (mean ± SE). Different alphabet indicate significant difference between species (p < 0.05). AC, Agropyron cristatum; BI, Bromus inermis; ED, Elymus dahuricus; FE, Festuca elataa; MS, Medicago sativa var. luxurians; OP, Onobrychis pulchell. Chl, Chlorophyll.

Fig. 2 Changes of chlorophyll fluorescence parameters of pasture in two habitats (mean ± SE). Different alphabet indicate significant difference between species (p < 0.05). AC, Agropyron cristatum; BI, Bromus inermis; ED, Elymus dahuricus; FE, Festuca elata; MS, Medicago sativa var. luxurians; OP, Onobrychis pulchella. Fm, maximum fluorescence yield; Fo, minimum fluorescence yield; Fv, variable fluorescence yield; Fv/Fm, maximum photochemical efficiency of PSII; Fv/Fo, potential activity of PSII.

Fig. 3 Changes of energy flow distribute in PSII reaction center of pasture in two habitats (mean ± SE). Different alphabet indicate significant difference between species (p < 0.05). AC, Agropyron cristatum; BI, Bromus inermis; ED, Elymus dahuricus; FE, Festuca elata; MS, Medicago sativa var. luxurians; OP, Onobrychis pulchella. The specific energy fluxes (per reaction centers, RC) for absorption (ABS/RC), trapping (TRo/RC), and dissipation (DIo/RC); RC/CSo, active reaction centers per cross-section; Mo, initial slope of fluorescence intensity.

Fig. 4 Changes of chlorophyll fluorescence transients fo pasture in two habitats. A, Kunlun Mountains. B, Cele oasis. The meaning of each point referred Li et al. (2005).

Table 1 The two-way ANOVA of chlorophyll content and fluorescence parameters of six pasture species in two habitats

参数 Parameter	变异来源 Source of variation	自由度 df	平方和 SS	均方 Mean square	F	p
叶绿素a Chlorophyll a	生境 Habitat	1	1.081 81	1.081 81	161.71	0.000^**
	物种 Species	5	3.373 93	0.674 79	100.87	0.000^**
	生境×物种 Interaction	5	2.363 28	0.472 66	70.65	0.000^**
	误差 Error	24	0.160 55	0.006 69
	总变异 Total variation	35	6.979 58
叶绿素b Chlorophyll b	生境 Habitat	1	0.306 63	0.306 63	272.80	0.000^**
	物种 Species	5	0.339 89	0.067 98	60.48	0.000^**
	生境×物种 Interaction	5	0.409 85	0.081 97	72.93	0.000^**
	误差 Error	24	0.026 98	0.001 12
	总变异 Total variation	35	1.083 36
总叶绿素 Chlorophyll	生境 Habitat	1	2.540 3	2.540 3	212.47	0.000^**
	物种 Species	5	5.848 1	1.169 6	97.83	0.000^**
	生境×物种 Interaction	5	4.683 2	0.936 6	78.34	0.000^**
	误差 Error	24	0.286 9	0.012 0
	总变异 Total variation	35	13.358 7
初始荧光 F_o	生境 Habitat	1	504 214	530 337	61.73	0.000^**
	物种 Species	5	424 867	84 734	9.86	0.000^**
	生境×物种 Interaction	5	293 673	58 735	6.84	0.000^**
	误差 Error	34	292 116	8 592
	总变异 Total variation	45	1 514 871
最大荧光 F_m	生境 Habitat	1	14 193 581	14 403 083	180.04	0.000^**
	物种 Species	5	1 616 251	339 166	4.24	0.004^**
	生境×物种 Interaction	5	2 440 896	488 179	6.84	0.000^**
	误差 Error	34	2 719 966	79 999
	总变异 Total variation	45	20 970 694
最大光化学效率 F_v/F_m	生境 Habitat	1	0.594 401	0.624 560	166.16	0.000^**
	物种 Species	5	0.259 409	0.053 059	14.12	0.000^**
	生境×物种 Interaction	5	0.232 687	0.046 537	12.38	0.000^**
	误差 Error	34	0.127 798	0.003 759
	总变异 Total variation	45	1.214 295

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