Chin J Plan Ecolo ›› 2015, Vol. 39 ›› Issue (3): 275-282.doi: 10.17521/cjpe.2015.0027

• Orginal Article • Previous Articles     Next Articles

Eco-physiological responses of Polytrichum commune to soil contamination by polychlorinated biphenyls

WU Qi-Mei, ZHOU Qi-Xing*()   

  1. Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education / Tianjin Key Laboratory of Remediation and Pollution Control for Urban Ecological Environment, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
  • Online:2015-03-17 Published:2015-03-01
  • Contact: Qi-Xing ZHOU E-mail:zhouqx@nankai.edu.cn
  • About author:

    # Co-first authors

Abstract: <i>Aims</i>

The objective of this study was to investigate the tolerance of Polytrichum commune to soil contamination by polychlorinated biphenyls (PCBs), and the underlying eco-physiological mechanisms.

<i>Methods</i>

Polytrichum commune was propagated and cultured in PCBs contaminated soil by means of micropropagation under laboratory conditions, and eco-physiological variables relating to tolerance to PCBs (Aroclor 1242 and Aroclor 1254) contamination were studied.

<i>Important findings</i>

The coverage and density of P. commune were higher than 93% and 50 plants·cm-2 and there was no significant difference between the PCBs treatments and the control. PCBs (Aroclor 1242 and Aroclor 1254) had no adverse effects on the regeneration of gametophyte fragments. The height and fresh mass of P. commune increased with an increase in the concentration of Aroclor 1242, and decreased with an increase in the concentration of Aroclor1254, but they were higher in the PCBs treatment than in the control, indicating that PCBs stimulated the growth of P. commune. The contents of chlorophyll (Chl) a, Chl b and Chl a + b in P. commune were slight higher in the PCBs treatments than in the control, and the ratio of Chl a/b maintained relatively stable in both the PCBs treatments and the control. The content of malondialdehyde and activity of superoxide dismutase in P. commune were not significantly different between the PCBs treatments and the control, whereas the content of glutathione was significantly higher in the PCBs treatments than in the control, indicating that glutathione played a pivotal role in the course of scavenging reactive oxygen species. The study demonstrated that the growth of P. commune was not adversely impacted by PCBs contaminated soil at given concentrations, reflecting its tolerance to PCBs.

Key words: eco-physiological response, pollution, polychlorinated biphenyls, Polytrichum commune

Fig. 1

Effects of different concentration (mg·kg-1) polychlorinated biphenyls (PCBs) contamination on the growth of Polytichum commune. A, Aroclor 1242 contaminated 1 month. B, Aroclor 1254 contaminated 1 month. C, Aroclor 1242 contaminated 6 months. D, Aroclor 1254 contaminated 6 months."

Fig. 2

Effects of polychlorinated biphenyls (PCBs) contamination on the coverage and density of Polytichum commune (mean ± SD)."

Fig. 3

Effects of polychlorinated biphenyls (PCBs) contamination on the height (A) and fresh mass (B) of Polytichum commune (mean ± SD). Different lowercase letters indicate significant differences among different Aroclor 1242 treatments (p < 0.05). Different capital letters indicate significant differences among different Aroclor 1254 treatments (p < 0.05)."

Table 1

Effects of polychlorinated biphenyls (PCBs) contamination on the content of chlorophyll (Chl) in Polytichum commune (mean ± SD)"

污染物
Pollutant
浓度
Concentration
(μg·kg-1)
Chl a
(mg·g-1)
处理/对照
Treatment /
control (%)
Chl b
(mg·g-1)
处理/对照
Treatment /
control (%)
Chl a + b
(mg·g-1)
处理/对照
Treatment /
control (%)
Chl a / b 处理/对照
Treatment /
Control (%)
CK 2.32 ± 0.13aA 100.00 0.92 ± 0.05aA 100.00 3.23 ± 0.18aA 100.00 2.52 ± 0.01aA 100.00
Aroclor
1242
5 2.78 ± 0.29ab 119.71 1.09 ± 0.12a 118.98 3.87 ± 0.41a 119.51 2.54 ± 0.02a 100.67
10 2.54 ± 0.10ab 109.60 1.00 ± 0.05a 108.51 3.54 ± 0.15a 109.29 2.55 ± 0.03a 101.03
20 2.97 ± 0.54b 128.04 1.16 ± 0.22a 125.85 4.12 ± 0.76a 127.42 2.57 ± 0.04a 101.91
Aroclor
1254
5 2.55 ± 0.16AB 109.99 0.99 ± 0.07AB 107.18 3.53 ± 0.22AB 109.20 2.59 ± 0.03B 102.66
10 2.77 ± 0.33B 119.39 1.10 ± 0.13B 119.56 3.86 ± 0.46B 119.44 2.52 ± 0.03A 99.88
20 2.35 ± 0.02A 101.50 0.91 ± 0.01A 98.75 3.26 ± 0.03A 100.72 2.59 ± 0.00B 102.79

Fig. 4

Effects of polychlorinated biphenyls (PCBs) contamination on the content of malondialdehyde (MDA) in Polyti- chum commune (mean ± SD)."

Fig. 5

Effects of polychlorinated biphenyls (PCBs) contamination on superoxide dismutase (SOD) activity (A) and glutathione (GSH) content (B) in Polytichum commune (mean ± SD). Different lowercase letters indicate significant differences among different Aroclor 1242 treatments (p < 0.05). Different capital letters indicate significant differences among different Aroclor 1254 treatments (p < 0.05)."

1 Alkorta I, Garbisu C (2001). Phytoremediation of organic contaminants in soils.Bioresource Technology, 79, 273-276.
2 Chekol T, Vough LR, Chaney RL (2004). Phytoremediation of polychlorinated biphenyl-contaminated soils: The rhizos- phere effect.Environment International, 30, 799-804.
3 Dalla Valle M, Jurado E, Dachs J, Sweetman AJ, Jones KC (2005). The maximum reservoir capacity of soils for persistent organic pollutants: Implications for global cycling.Environmental Pollution, 134, 153-164.
4 Ding N, Hayat T, Wang J, Wang HZ, Liu XM, Xu JM (2011). Responses of microbial community in rhizosphere soils when ryegrass was subjected to stress from PCBs.Journal of Soils and Sediments, 11, 1355-1362.
5 Gao JF (2006). Experimental Guidance for Plant Physiology. Higher Education Press, Beijing. 210-211. (in Chinese)
[高俊凤 (2006). 植物生理学实验指导. 高等教育出版社, 北京. 210-211.]
6 Huang XD, El-Alawi Y, Penrose DM, Glick BR, Greenberg BM (2004). Responses of three grass species to creosote during phytoremediation.Environmental Pollution, 130, 453-463.
7 Jia Y, Bai XL, Shan FB, Bai SW, Zhong HR (2012). Experiment of artificially culturing moss crust and its maintenance mechanism.Journal of Desert Research, 32, 54-59. (in Chinese with English abstract)
[贾艳, 白学良, 单飞彪, 白少伟, 詹洪瑞 (2012). 藓类结皮层人工培养试验和维持机制研究. 中国沙漠, 32, 54-59.]
8 Kauss PB, Hutchinson TC (1975). The effects of water-soluble petroleum components on the growth of Chlorella vulgaris Beijerinck.Environmental Pollution, 9, 157-174.
9 Li Y, Liang F, Zhu YF, Wang FP (2013). Phytoremediation of a PCB-contaminated soil by alfalfa and tall fescue single and mixed plants cultivation.Journal of Soils and Sediments, 13, 925-931.
10 Liu YY, Sun HB, Chen GZ (2006). Effects of polychlorinated biphenys on the growth of mangrove species, Aegiceras corniculatum.Acta Scientiarum Naturalium Universitatis Sunyatseni, 45(9), 108-112. (in Chinese with English abstract)
[刘亚云, 孙红斌, 陈桂珠 (2006). 多氯联苯(PCBs)污染对桐花树幼苗生长的影响. 中山大学学报(自然科学版), 45(9), 108-112.]
11 Liu YY, Sun HB, Chen GZ (2007a). Effects of PCBs on the growth of mangrove species, Kandelia candel.Marine Environmental Science, 26, 23-27. (in Chinese with English abstract)
[刘亚云, 孙红斌, 陈桂珠 (2007a). 多氯联苯(PCBs)污染对秋茄Kandelia candel生长的影响. 海洋环境科学, 26, 23-27.]
12 Liu YY, Sun HB, Chen GZ, Zhao B, Li WY (2007b). Eco-physiological responses of Kandelia candel seedlings to polychlorinated biphenyls (PCBs) treatment.Acta Ecologica Sinica, 27, 746-754. (in Chinese with English abstract)
[刘亚云, 孙红斌, 陈桂珠, 赵波, 李伟煜 (2007b). 秋茄(Kandelia candel)幼苗对多氯联苯污染的生理生态响应. 生态学报, 27, 746-754.]
13 Maliszewska-Kordybach B, Smreczak B (2000). Ecotoxicological activity of soils polluted with polycyclic aromatic hydrocarbons (PAHs)—Effect on plants.Environmental Technology, 21, 1099-1110.
14 Ockenden WA, Breivik K, Meijer SN, Steinnes E, Sweetman AJ, Jones KC (2003). The global re-cycling of persistent organic pollutants is strongly retarded by soils.Environmental Pollution, 121, 75-80.
15 Qin H, Brookes PC, Xu JM (2014). Cucurbita spp. and Cucumis sativus enhance the dissipation of polychlorinated biphenyl congeners by stimulating soil microbial community development.Environmental Pollution, 184, 306-312.
16 Tian GQ, Bai XL, Xu J, Wang XD (2005). Experimental studies on natural regeneration and artificial cultures of moss crusts on fixed dunes in the Tengger Desert.Acta Phytoecologica Sinica, 29, 164-169. (in Chinese with English abstract)
[田桂泉, 白学良, 徐杰, 王先道 (2005). 腾格里沙漠固定沙丘藓类植物结皮层的自然恢复及人工培养试验研究. 植物生态学报, 29, 164-169.]
17 Tu C, Teng Y, Luo YM, Sun XH, Deng SP, Li ZG, Liu WX, Xu ZH (2011). PCB removal, soil enzyme activities, and microbial community structures during the phytoremediation by alfalfa in field soils.Journal of Soils and Sediments, 11, 649-656.
18 Weber JB, Mrozek E Jr (1979). Polychlorinated biphenyls: Phytotoxicity, absorption and translocation by plants, and inactivation by activated carbon.Bulletin of Environmental Contamination and Toxicology, 23, 412-417.
19 Zeeb BA, Amphlett JS, Rutter A, Reimer KJ (2006). Potential for phytoremediation of polychlorinated biphenyl-(PCB)contaminated soil. International Journal of Phytoremediation, 8, 199-221.
20 Zhang XZ (1986). Plant chlorophyll content determination: Acetone ethanol mixture method.Journal of Liaoning Agricultural Sciences, (3), 26-28. (in Chinese)
[张宪政 (1986). 植物叶绿素含量测定——丙酮乙醇混合液法. 辽宁农业科学, (3), 26-28.]
22 Zhou JJ, Hu LL, Li M, Chen WF, Wang Y, Wang HC (2013). Effects of PCBs pollution on rape (Brassica campestris) growth and their accumulation in Brassica campestris.Journal of Agro-Environment Science, 32, 1959-1964. (in Chinese with English abstract)
[周佳佳, 胡林林, 李敏, 陈为峰, 王莹, 王海翠 (2013). 多氯联苯(PCBs)污染对油菜(Brassica campestris)生长的影响及积累效应研究. 农业环境科学学报, 32, 1959-1964.]
23 Zhou QX, Cai Z, Zhang ZN, Liu WT (2011). Ecological remediation of hydrocarbon contaminated soils with weed plant.Journal of Resources and Ecology, 2, 97-105.
24 Zhou QX, Diao CY, Sun YB, Zhou JL (2012). Tolerance, uptake and removal of nitrobenzene by a newly-found remediation species Mirabilis jalapa L.Chemosphere, 86, 994-1000.
25 Zhou QX, Kong FX, Zhu L (2004). Ecotoxicology. Science Press, Beijing. (in Chinese)
[周启星, 孔繁翔, 朱琳 (2004). 生态毒理学. 科学出版社, 北京.]
26 Zhou QX, Luo Y (2011). Pollution Eco-chemistry. Science Press, Beijing. 30-35. (in Chinese)
[周启星, 罗义 (2011). 污染生态化学. 科学出版社, 北京. 30-35.]
27 Zhou QX, Wei SH, Diao CY (2007). Basic principles and researching progresses in ecological remediation of contaminated soils.Journal of Agro-environment Science, 26, 419-424. (in Chinese with English abstract)
[周启星, 魏树和, 刁春燕 (2007). 污染土壤生态修复基本原理及研究进展. 农业环境科学学报, 26, 419-424.]
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